WormBase Tree Display for Variation: WBVar00251849

WBVar00251849 Name: Public_name: tm3067
    Other_name: C05C8.9b.2:c.-43_345del
      C05C8.9a.1:c.-43_345del
      C05C8.9b.1:c.-43_345del
    HGVSg: CHROMOSOME_V:g.7237515_7238007del
  Sequence_details: SMap: S_parent: Sequence: C05C8
    Flanking_sequences: gtagtttaacgtgtacgtcatttcctcctt cctgagagaaaccttctggtcgatcgagcg
    Mapping_target: C05C8
    Source_location: 7 CHROMOSOME_V 7237514 7238008 Inferred_automatically: National_Bioresource_Project
    Type_of_mutation: Deletion
    PCR_product: tm3067_external
      tm3067_internal
    SeqStatus: Sequenced
  Variation_type: Allele
  Origin: Species: Caenorhabditis elegans
    Laboratory: FX
    Author: Mitani S
    DB_info: Database: National_Bioresource_Project seq 3067
    NBP_allele
    Status: Live
  Affects: Gene: WBGene00015466
    Transcript: C05C8.9b.1 VEP_consequence: splice_acceptor_variant,splice_donor_variant,start_lost,5_prime_UTR_variant,intron_variant
        VEP_impact: HIGH
        HGVSc: C05C8.9b.1:c.-43_345del
        cDNA_position: 103-490
        CDS_position: ?-345
        Protein_position: ?-115
        Intron_number: 2-3/5
        Exon_number: 1-4/6
      C05C8.9a.1 VEP_consequence: splice_acceptor_variant,splice_donor_variant,start_lost,5_prime_UTR_variant,intron_variant
        VEP_impact: HIGH
        HGVSc: C05C8.9a.1:c.-43_345del
        cDNA_position: 135-522
        CDS_position: ?-345
        Protein_position: ?-115
        Intron_number: 2-3/6
        Exon_number: 1-4/7
      C05C8.9b.2 VEP_consequence: splice_acceptor_variant,splice_donor_variant,start_lost,5_prime_UTR_variant,intron_variant
        VEP_impact: HIGH
        HGVSc: C05C8.9b.2:c.-43_345del
        cDNA_position: 100-487
        CDS_position: ?-345
        Protein_position: ?-115
        Intron_number: 2-3/4
        Exon_number: 1-4/5
  Isolation: Mutagen: TMP/UV
  Genetics: Map: V
  Description: Phenotype: WBPhenotype:0000238 Paper_evidence: WBPaper00035062
        Curator_confirmed: WBPerson712
        Remark: Foraging behavior was reduced compared to control Paper_evidence: WBPaper00035062
            Curator_confirmed: WBPerson712
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00035062
            Curator_confirmed: WBPerson712
      WBPhenotype:0000304 Paper_evidence: WBPaper00035062
        Curator_confirmed: WBPerson712
        Remark: Chemotaxis to isoamyl alcohol was reduced compared to control Paper_evidence: WBPaper00035062
            Curator_confirmed: WBPerson712
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00035062
            Curator_confirmed: WBPerson712
      WBPhenotype:0000310 Paper_evidence: WBPaper00035062
        Curator_confirmed: WBPerson712
        Remark: Residual cilia were not associated with a particular type of neuron; The ASER neuron lacks ciliary projections Paper_evidence: WBPaper00035062
            Curator_confirmed: WBPerson712
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00035062
            Curator_confirmed: WBPerson712
      WBPhenotype:0000425 Paper_evidence: WBPaper00035062
        Curator_confirmed: WBPerson712
        Remark: Punctate HYLS-1 staining was absent in hyls-1deletion worms Paper_evidence: WBPaper00035062
            Curator_confirmed: WBPerson712
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00035062
            Curator_confirmed: WBPerson712
      WBPhenotype:0000436 Paper_evidence: WBPaper00035062
        Curator_confirmed: WBPerson712
        Remark: CHE-11:GFP fails to accumulate at the transition zone of hyls-1 mutants Paper_evidence: WBPaper00035062
            Curator_confirmed: WBPerson712
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00035062
            Curator_confirmed: WBPerson712
      WBPhenotype:0000843 Paper_evidence: WBPaper00035062
        Curator_confirmed: WBPerson712
        Remark: Male mating efficiency was reduced compared to control Paper_evidence: WBPaper00035062
            Curator_confirmed: WBPerson712
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00035062
            Curator_confirmed: WBPerson712
      WBPhenotype:0002535 Paper_evidence: WBPaper00035062
        Person_evidence: WBPerson7743
        Curator_confirmed: WBPerson712
        Remark: In hyls-1 mutant animals, only 10% of the normal number of ciliated neurons were observed to dye-fill Paper_evidence: WBPaper00035062
            Curator_confirmed: WBPerson712
          Comment to the National Bioresource of Japan from Dr. O. Blacque: partially Dyf. Person_evidence: WBPerson7743
            Curator_confirmed: WBPerson712
            Laboratory_evidence: OEB
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00035062
            Curator_confirmed: WBPerson712
    Phenotype_not_observed: WBPhenotype:0000062 Paper_evidence: WBPaper00035062
        Person_evidence: WBPerson7743
        Curator_confirmed: WBPerson712
        Remark: Classified as homozygous viable by the National Bioresource Project of Japan. Comment to the NBP from Dr. K. Oegema: Genes & Dev. 23, 2046 (2009). Person_evidence: WBPerson7743
            Curator_confirmed: WBPerson712
            Laboratory_evidence: FX
              OD
          hyls-1 mutant embryos were viable Paper_evidence: WBPaper00035062
            Curator_confirmed: WBPerson712
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00035062
            Curator_confirmed: WBPerson712
      WBPhenotype:0000688 Paper_evidence: WBPaper00035062
        Curator_confirmed: WBPerson712
        Remark: hyls-1 mutant embryos developed into fertile adults Paper_evidence: WBPaper00035062
            Curator_confirmed: WBPerson712
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00035062
            Curator_confirmed: WBPerson712
      WBPhenotype:0000853 Paper_evidence: WBPaper00035062
        Curator_confirmed: WBPerson712
        Remark: IFT is unchanged in residual cilia of hyls-1 mutant animals Paper_evidence: WBPaper00035062
            Curator_confirmed: WBPerson712
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00035062
            Curator_confirmed: WBPerson712
      WBPhenotype:0001333 Paper_evidence: WBPaper00035062
        Curator_confirmed: WBPerson712
        Remark: Neuron-specific GFP reporters confirmed normal presence of the amphid and phasmid neurons in hyls-1 mutants Paper_evidence: WBPaper00035062
            Curator_confirmed: WBPerson712
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00035062
            Curator_confirmed: WBPerson712
      WBPhenotype:0001588 Paper_evidence: WBPaper00035062
        Curator_confirmed: WBPerson712
        Remark: hyls-1 mutant embryos did not show centriole duplication defects Paper_evidence: WBPaper00035062
            Curator_confirmed: WBPerson712
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00035062
            Curator_confirmed: WBPerson712
  Disease_info: Models_disease: DOID:0050779
    Models_disease_in_annotation: WBDOannot00000206
  Reference: WBPaper00035062
  Remark: 13498/13499-13991/13992 (493 bp deletion)
    This knockout was generated by the National Bioresource Project, Tokyo, Japan, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence: WBPaper00041807
  Method: NBP_knockout_allele