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WormBase Tree Display for Variation: WBVar00250733

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Name Class

WBVar00250733NamePublic_nametm1768
Other_nameCE06191:p.Ile36_Ter128delinsLysLeuLeuLeuAlaLeu
M05B5.5a.1:c.107_384delinsAGCTGTTGTTGGCTTTA
HGVSgCHROMOSOME_I:g.7193174_7193801delinsTAAAGCCAACAACAGCT
Sequence_detailsSMapS_parentSequenceC01H6
Flanking_sequencesaacaatcgctgatgtttctgcagaattcgtttataaggataatcatatgcatttggaagc
Mapping_targetC01H6
Source_location7CHROMOSOME_I71931737193804Inferred_automaticallyNational_Bioresource_Project
Type_of_mutationInsertionTAAAGCCAACAACAGCTTA
Deletion
PCR_producttm1768_external
tm1768_internal
SeqStatusSequenced
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00022642
LaboratoryFX
AuthorMitani S
DB_infoDatabaseNational_Bioresource_Projectseq1768
NBP_allele
StatusLive
AffectsGeneWBGene00001949
TranscriptM05B5.5a.1 (11)
InteractorWBInteraction000052005
WBInteraction000052006
WBInteraction000052007
WBInteraction000501329
WBInteraction000503977
WBInteraction000517476
WBInteraction000517477
IsolationMutagenTMP/UV
GeneticsMapI
DescriptionPhenotypeWBPhenotype:0000184Person_evidenceWBPerson7743
Curator_confirmedWBPerson48
RemarkComment from Dr. B. Conradt to the National Bioresource Project of Japan: 3% NSM sister cell survival at 20C.Person_evidenceWBPerson7743
Curator_confirmedWBPerson48
Laboratory_evidenceMD
PenetranceLowPerson_evidenceWBPerson7743
Curator_confirmedWBPerson48
Range33Person_evidenceWBPerson7743
Curator_confirmedWBPerson48
EQ_annotationsAnatomy_termWBbt:0003666PATO:0000460Person_evidenceWBPerson7743
Curator_confirmedWBPerson48
WBPhenotype:0000188Paper_evidenceWBPaper00033102
Curator_confirmedWBPerson2021
RemarkGonadal arms were shortPaper_evidenceWBPaper00033102
Curator_confirmedWBPerson2021
RecessivePaper_evidenceWBPaper00033102
Curator_confirmedWBPerson2021
Variation_effectHypomorph_reduction_of_functionPaper_evidenceWBPaper00033102
Curator_confirmedWBPerson2021
Temperature_sensitiveHeat_sensitive25CPaper_evidenceWBPaper00033102
Curator_confirmedWBPerson2021
WBPhenotype:0000396Paper_evidenceWBPaper00033102
Curator_confirmedWBPerson2021
RemarkGonad arms were not reflexed in most gonadsPaper_evidenceWBPaper00033102
Curator_confirmedWBPerson2021
RecessivePaper_evidenceWBPaper00033102
Curator_confirmedWBPerson2021
Variation_effectHypomorph_reduction_of_functionPaper_evidenceWBPaper00033102
Curator_confirmedWBPerson2021
Temperature_sensitiveHeat_sensitive25CPaper_evidenceWBPaper00033102
Curator_confirmedWBPerson2021
WBPhenotype:0000684Paper_evidenceWBPaper00033102
Curator_confirmedWBPerson2021
RemarkA dramatic reduction in germ cell number was observed when hlh-2(tm1768) mutants were shifted to 25 CPaper_evidenceWBPaper00033102
Curator_confirmedWBPerson2021
Variation_effectHypomorph_reduction_of_functionPaper_evidenceWBPaper00033102
Curator_confirmedWBPerson2021
Temperature_sensitiveHeat_sensitive25CPaper_evidenceWBPaper00033102
Curator_confirmedWBPerson2021
Phenotype_assayTreatmentDAPI stainingPaper_evidenceWBPaper00033102
Curator_confirmedWBPerson2021
WBPhenotype:0000688Paper_evidenceWBPaper00039950
Person_evidenceWBPerson7743
Curator_confirmedWBPerson48
WBPerson712
RemarkComment to the National Bioresource Project of Japan from Dr. B. Conradt: sterile at 25C; Dr. J. Kimble: Dev. Biol. 331, 14 (2009).Person_evidenceWBPerson7743
Curator_confirmedWBPerson48
Laboratory_evidenceMD
The allele is fully sterile at 25 deg C and partially sterile at 20 deg C.Paper_evidenceWBPaper00039950
Curator_confirmedWBPerson712
Temperature_sensitiveHeat_sensitive25Paper_evidenceWBPaper00039950
Person_evidenceWBPerson7743
Curator_confirmedWBPerson48
WBPerson712
WBPhenotype:0000690Paper_evidenceWBPaper00033102
Curator_confirmedWBPerson2021
RemarkThis allele exhibited recessive defects in hermaphrodite gonad arm migration (data not shown)Paper_evidenceWBPaper00033102
Curator_confirmedWBPerson2021
RecessivePaper_evidenceWBPaper00033102
Curator_confirmedWBPerson2021
Variation_effectHypomorph_reduction_of_functionPaper_evidenceWBPaper00033102
Curator_confirmedWBPerson2021
Temperature_sensitiveHeat_sensitive25CPaper_evidenceWBPaper00033102
Curator_confirmedWBPerson2021
WBPhenotype:0000893Paper_evidenceWBPaper00033102
Curator_confirmedWBPerson2021
RemarkThe hlh-2(tm1768) males had a significantly smaller germline mitotic region than controlsPaper_evidenceWBPaper00033102
Curator_confirmedWBPerson2021
Variation_effectHypomorph_reduction_of_functionPaper_evidenceWBPaper00033102
Curator_confirmedWBPerson2021
Temperature_sensitiveHeat_sensitive25CPaper_evidenceWBPaper00033102
Curator_confirmedWBPerson2021
WBPhenotype:0001652Paper_evidenceWBPaper00039950
Curator_confirmedWBPerson712
RemarkAnimals exhibit a significant, 5%, defect in AC invasion at the P6.p four-cell stage. hlh-2(tm1768) animals with zero or299 two ACs were not observed. Defect is not stronger at higher temperatures.Paper_evidenceWBPaper00039950
Curator_confirmedWBPerson712
PenetranceLowPaper_evidenceWBPaper00039950
Curator_confirmedWBPerson712
WBPhenotype:0001656Paper_evidenceWBPaper00033102
Curator_confirmedWBPerson2021
RemarkWeakly dominant defects in hermaphrodite DTC specification. hlh-2 (tm1768 +RNAi) mutants at 25C show more severe DTC loss in hermaphrodites and linker cell loss in males as indicated by lag-2 and ceh-22 reportersPaper_evidenceWBPaper00033102
Curator_confirmedWBPerson2021
Semi_dominantPaper_evidenceWBPaper00033102
Curator_confirmedWBPerson2021
Variation_effectHypomorph_reduction_of_functionPaper_evidenceWBPaper00033102
Curator_confirmedWBPerson2021
EQ_annotationsLife_stageWBls:0000057PATO:0000460Paper_evidenceWBPaper00033102
Curator_confirmedWBPerson2021
WBls:0000056PATO:0000460Paper_evidenceWBPaper00033102
Curator_confirmedWBPerson2021
Temperature_sensitiveHeat_sensitive25CPaper_evidenceWBPaper00033102
Curator_confirmedWBPerson2021
Phenotype_assayGenotypeqIs57[lag-2::GFP] , qIs56[lag-2::GFP], qIs90[ceh- 22b::VENUS]Paper_evidenceWBPaper00033102
Curator_confirmedWBPerson2021
Phenotype_not_observedWBPhenotype:0000062Person_evidenceWBPerson7743
Curator_confirmedWBPerson48
RemarkClassified as homozygous viable by the National Bioresource Project of Japan.Person_evidenceWBPerson7743
Curator_confirmedWBPerson48
Laboratory_evidenceFX
ReferenceWBPaper00039950
WBPaper00033102
Remark[C01H6] 1369/1370-TAAAGCCAACAACAGCTTA-[C01H6] 1999/2000 (630 bp deletion + 19 bp insertion)
This knockout was generated by the National Bioresource Project, Tokyo, Japan, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.Paper_evidenceWBPaper00041807
MethodNBP_knockout_allele