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WormBase Tree Display for Variation: WBVar00250355

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Name Class

WBVar00250355NamePublic_nametm1361
Other_nameW09B6.3a.1:c.535-147_679del
HGVSgCHROMOSOME_II:g.1124893_1125184del
Sequence_detailsSMapS_parentSequenceW09B6
Flanking_sequencesttttcgttcaaaatatcagttttaaacctgtcatcacaaaaattatgaaaaatcatttta
Mapping_targetW09B6
Source_location7CHROMOSOME_II11248921125185Inferred_automaticallyNational_Bioresource_Project
Type_of_mutationDeletion
PCR_producttm1361_external
tm1361_internal
SeqStatusSequenced
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00040457
LaboratoryFX
AuthorMitani S
DB_infoDatabaseNational_Bioresource_Projectseq1361
NBP_allele
StatusLive
AffectsGeneWBGene00021103
TranscriptW09B6.3a.1VEP_consequencesplice_acceptor_variant,coding_sequence_variant,intron_variant
VEP_impactHIGH
HGVScW09B6.3a.1:c.535-147_679del
cDNA_position?-681
CDS_position?-679
Protein_position?-227
Intron_number5/12
Exon_number6/13
W09B6.3b.1VEP_consequencecoding_sequence_variant,5_prime_UTR_variant
VEP_impactMODIFIER
cDNA_position?-121
CDS_position?-121
Protein_position?-41
Exon_number1/7
IsolationMutagenTMP/UV
GeneticsMapII
DescriptionPhenotypeWBPhenotype:0000688Paper_evidenceWBPaper00038150
Curator_confirmedWBPerson712
Temperature_sensitiveHeat_sensitive25Paper_evidenceWBPaper00038150
Curator_confirmedWBPerson712
WBPhenotype:0000983Paper_evidenceWBPaper00044616
Curator_confirmedWBPerson602
Remarkfer-3(hc3) fails to complement the Fer and Eri phenotypes of eri-3(tm1361).Paper_evidenceWBPaper00044616
Curator_confirmedWBPerson602
WBPhenotype:0001175Paper_evidenceWBPaper00027057
Curator_confirmedWBPerson712
Remarkeri mutant strains exhibit an increase in the frequency of X chromosome nondisjunction resulting in a high incidence of males (Him) phenotype.Paper_evidenceWBPaper00027057
Curator_confirmedWBPerson712
WBPhenotype:0001258Paper_evidenceWBPaper00038150
WBPaper00027057
WBPaper00044616
Curator_confirmedWBPerson712
WBPerson602
RemarkTissue and RNA-specific enhancement. Exhibits an Eri phenotype that has an upper bound of 95% confidence interval at least 100% penetrant with a ~10% standard deviation in the pharynx.Paper_evidenceWBPaper00038150
Curator_confirmedWBPerson712
in wild-type animals, RNAi targeting unc-73 results in a low-penetrance-uncoordinated (Unc) phenotype, such that only about 4% of the F1 progeny of animals exposed to unc-73(RNAi) exhibit the characteristic deformed, poorly motile phenotype. In contrast, these mutants exhibited enhanced RNAi against unc-73. RNAi targeting lin-1, dpy-13, hmr-1, and a neuronal unc-47::gfp was also enhanced in these mutants.Paper_evidenceWBPaper00027057
Curator_confirmedWBPerson712
fer-3(hc3) fails to complement the Fer and Eri phenotypes of eri-3(tm1361).Paper_evidenceWBPaper00044616
Curator_confirmedWBPerson602
WBPhenotype:0001369Paper_evidenceWBPaper00027057
Curator_confirmedWBPerson712
RemarkThe interaction between DCR-1 and ERI-1 was abolished in eri-3 mutant extracts.Paper_evidenceWBPaper00027057
Curator_confirmedWBPerson712
WBPhenotype:0001595Paper_evidenceWBPaper00027057
Curator_confirmedWBPerson712
Remarkeri mutant strains exhibit spontaneous silencing of simple transgenic arrays in the soma.Paper_evidenceWBPaper00027057
Curator_confirmedWBPerson712
WBPhenotype:0001683Paper_evidenceWBPaper00027057
Curator_confirmedWBPerson712
Remarkeri mutant strains exhibit a sperm-defective sterile phenotype at 25 degC.Paper_evidenceWBPaper00027057
Curator_confirmedWBPerson712
Temperature_sensitiveHeat_sensitive25Paper_evidenceWBPaper00027057
Curator_confirmedWBPerson712
WBPhenotype:0002147Paper_evidenceWBPaper00027057
Curator_confirmedWBPerson712
RemarkAccumulation of several small-RNA species, including small RNAs corresponding to the somatically expressed gene K02E2.6, the X cluster, and the tncRNAs species required ERI-3 proteins. The eri mutant strains exhibited defects in the accumulation of these small-RNA species even when maintained at the permissive temperature. A 5-fold upregulation of K02E2.6 mRNA levels was observed.Paper_evidenceWBPaper00027057
Curator_confirmedWBPerson712
Phenotype_not_observedWBPhenotype:0000062Person_evidenceWBPerson7743
Curator_confirmedWBPerson712
RemarkClassified as homozygous viable by the National BioResource Project of Japan. Dr. C. Mello, Cell 124, 343-354 (2006). Comment from Dr. C. Bargmann to the National Bioresource Project of Japan: viable.Person_evidenceWBPerson7743
Curator_confirmedWBPerson712
WBPhenotype:0000688Person_evidenceWBPerson7743
Curator_confirmedWBPerson712
RemarkComment from Dr. C. Bargmann to the National Bioresource Project of Japan: fertile at 20 CPerson_evidenceWBPerson7743
Curator_confirmedWBPerson712
WBPhenotype:0002146Paper_evidenceWBPaper00027057
Curator_confirmedWBPerson712
RemarkThe accumulation of at least eight small RNAs corresponding to germline-expressed genes, including T01A4.3, did not appear to require this gene.Paper_evidenceWBPaper00027057
Curator_confirmedWBPerson712
ReferenceWBPaper00038150
WBPaper00027057
WBPaper00044616
Remark2085/2086-2377/2378 (292 bp deletion)
This knockout was generated by the National Bioresource Project, Tokyo, Japan, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.Paper_evidenceWBPaper00041807
MethodNBP_knockout_allele