WormBase Tree Display for Variation: WBVar00249136
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WBVar00249136 | Evidence | Paper_evidence | WBPaper00018640 | |||||
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Name | Public_name | t1545 | ||||||
Sequence_details | SeqStatus | Pending_curation | ||||||
Variation_type | Allele | |||||||
Origin | Species | Caenorhabditis elegans | ||||||
Strain | WBStrain00007763 | |||||||
WBStrain00007806 | ||||||||
Laboratory | GE | |||||||
Status | Live | |||||||
Affects | Gene | WBGene00006571 | ||||||
Description | Phenotype | WBPhenotype:0000313 | Paper_evidence | WBPaper00005919 | ||||
Curator_confirmed | WBPerson712 | |||||||
Remark | tim-1(ts) mutants had an extended transition zone in which polarized nuclei persisted into the region of the gonad that is normally filled with pachytene nuclei. | Paper_evidence | WBPaper00005919 | |||||
Curator_confirmed | WBPerson712 | |||||||
Temperature_sensitive | Heat_sensitive | 25 | Paper_evidence | WBPaper00005919 | ||||
Curator_confirmed | WBPerson712 | |||||||
WBPhenotype:0000436 | Paper_evidence | WBPaper00005919 | ||||||
Curator_confirmed | WBPerson712 | |||||||
Remark | SYP-1 localization was aberrant in tim-1(ts) mutants. SYP-1 assembled in short discontinuous patches along chromosomes of most tim-1(ts) pachytene-region nuclei (85%, n=62), rather than continuously along homologous chromosomes, as in wild-type nuclei. In contrast, the co-staining control marker SMC-1 localized properly. | Paper_evidence | WBPaper00005919 | |||||
Curator_confirmed | WBPerson712 | |||||||
Temperature_sensitive | Heat_sensitive | 25 | Paper_evidence | WBPaper00005919 | ||||
Curator_confirmed | WBPerson712 | |||||||
WBPhenotype:0000773 | Paper_evidence | WBPaper00003469 | ||||||
Curator_confirmed | WBPerson557 | |||||||
WBPhenotype:0000775 | Paper_evidence | WBPaper00005919 | ||||||
Curator_confirmed | WBPerson712 | |||||||
Remark | The tim-1 allele selectively disrupts meiosis. | Paper_evidence | WBPaper00005919 | |||||
Curator_confirmed | WBPerson712 | |||||||
WBPhenotype:0001215 | Paper_evidence | WBPaper00005919 | ||||||
Curator_confirmed | WBPerson712 | |||||||
Remark | However, under a specific temperature regime, the tim-1 allele selectively disrupts meiosis but not germline mitosis. | Paper_evidence | WBPaper00005919 | |||||
Curator_confirmed | WBPerson712 | |||||||
Temperature_sensitive | Heat_sensitive | 25 | Paper_evidence | WBPaper00005919 | ||||
Curator_confirmed | WBPerson712 | |||||||
WBPhenotype:0001400 | Paper_evidence | WBPaper00005919 | ||||||
Curator_confirmed | WBPerson712 | |||||||
Remark | In tim-1 mutants, SCC-3 concentrations were variably reduced, and in half the gonads the residual protein was undetectable on the meiotic chromosomes. | Paper_evidence | WBPaper00005919 | |||||
Curator_confirmed | WBPerson712 | |||||||
Temperature_sensitive | Heat_sensitive | 25 | Paper_evidence | WBPaper00005919 | ||||
Curator_confirmed | WBPerson712 | |||||||
WBPhenotype:0001408 | Paper_evidence | WBPaper00005919 | ||||||
Curator_confirmed | WBPerson712 | |||||||
Remark | REC-8 was not detectable on tim-1(ts) chromosomes (data not shown) in meiotic prophase, although it was present in pre-meiotic nuclei. | Paper_evidence | WBPaper00005919 | |||||
Curator_confirmed | WBPerson712 | |||||||
Temperature_sensitive | Heat_sensitive | 25 | Paper_evidence | WBPaper00005919 | ||||
Curator_confirmed | WBPerson712 | |||||||
WBPhenotype:0001814 | Paper_evidence | WBPaper00005919 | ||||||
Curator_confirmed | WBPerson712 | |||||||
Remark | Homologous chromosomes of tim-1(ts) mutants rarely formed chiasmata. Of 142 diakinesis nuclei from wild-type germlines, all had six bivalents. Instead, 101 of 105 tim-1(ts) nuclei had 7-12 staining bodies, with an average of 10; they were mostly univalents and an occasional bivalent, indicating disrupted homologue linkage. | Paper_evidence | WBPaper00005919 | |||||
Curator_confirmed | WBPerson712 | |||||||
Temperature_sensitive | Heat_sensitive | 25 | Paper_evidence | WBPaper00005919 | ||||
Curator_confirmed | WBPerson712 | |||||||
WBPhenotype:0002013 | Paper_evidence | WBPaper00005919 | ||||||
Curator_confirmed | WBPerson557 | |||||||
Remark | Homologous chromosomes of tim-1(ts) mutants rarely formed chiasmata. Of 142 diakinesis nuclei from wild-type germlines, all had six bivalents. Instead, 101 of 105 tim-1(ts) nuclei had 7-12 staining bodies, with an average of 10; they were mostly univalents and an occasional bivalent, indicating disrupted homologue linkage. | Paper_evidence | WBPaper00005919 | |||||
Curator_confirmed | WBPerson557 | |||||||
Temperature_sensitive | Heat_sensitive | 25 | Paper_evidence | WBPaper00005919 | ||||
Curator_confirmed | WBPerson557 | |||||||
WBPhenotype:0002113 | Paper_evidence | WBPaper00005919 | ||||||
Curator_confirmed | WBPerson712 | |||||||
Remark | Although meiotic chromosomes of tim-1(ts) mutants reorganized into a pachytene-like arrangement just before diplotene like those of syp-1 mutants, they failed to achieve the normal lengthwise alignment typical of synapsed pachytene chromosomes. Only 17% (n = 65) of tim-1(ts) nuclei within the late pachytene region had one 5S rDNA FISH signal, in comparison with 89% (n = 93) of wild-type nuclei, indicating a severe defect in synapsis. | Paper_evidence | WBPaper00005919 | |||||
Curator_confirmed | WBPerson712 | |||||||
Temperature_sensitive | Heat_sensitive | 25 | Paper_evidence | WBPaper00005919 | ||||
Curator_confirmed | WBPerson712 | |||||||
Phenotype_not_observed | WBPhenotype:0001882 | Paper_evidence | WBPaper00005919 | |||||
Curator_confirmed | WBPerson712 | |||||||
Remark | Pre-meiotic nuclei from tim-1(t1545ts) and wild-type animals grown at 25 deg C were indistinguishable. | Paper_evidence | WBPaper00005919 | |||||
Curator_confirmed | WBPerson712 | |||||||
WBPhenotype:0001952 | Paper_evidence | WBPaper00005919 | ||||||
Curator_confirmed | WBPerson712 | |||||||
Remark | Transition-zone nuclei of tim-1(ts) mutants had normal polarized organization and a similar proportion of paired and unpaired homologues to that in wild-type nuclei, on the basis of 5S rDNA FISH signals. | Paper_evidence | WBPaper00005919 | |||||
Curator_confirmed | WBPerson712 | |||||||
Temperature_sensitive | Heat_sensitive | 25 | Paper_evidence | WBPaper00005919 | ||||
Curator_confirmed | WBPerson712 | |||||||
Reference | WBPaper00018640 | |||||||
WBPaper00005919 | ||||||||
WBPaper00003469 | ||||||||
Method | Allele |