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WormBase Tree Display for Variation: WBVar00249136

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Name Class

WBVar00249136EvidencePaper_evidenceWBPaper00018640
NamePublic_namet1545
Sequence_detailsSeqStatusPending_curation
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00007763
WBStrain00007806
LaboratoryGE
StatusLive
AffectsGeneWBGene00006571
DescriptionPhenotypeWBPhenotype:0000313Paper_evidenceWBPaper00005919
Curator_confirmedWBPerson712
Remarktim-1(ts) mutants had an extended transition zone in which polarized nuclei persisted into the region of the gonad that is normally filled with pachytene nuclei.Paper_evidenceWBPaper00005919
Curator_confirmedWBPerson712
Temperature_sensitiveHeat_sensitive25Paper_evidenceWBPaper00005919
Curator_confirmedWBPerson712
WBPhenotype:0000436Paper_evidenceWBPaper00005919
Curator_confirmedWBPerson712
RemarkSYP-1 localization was aberrant in tim-1(ts) mutants. SYP-1 assembled in short discontinuous patches along chromosomes of most tim-1(ts) pachytene-region nuclei (85%, n=62), rather than continuously along homologous chromosomes, as in wild-type nuclei. In contrast, the co-staining control marker SMC-1 localized properly.Paper_evidenceWBPaper00005919
Curator_confirmedWBPerson712
Temperature_sensitiveHeat_sensitive25Paper_evidenceWBPaper00005919
Curator_confirmedWBPerson712
WBPhenotype:0000773Paper_evidenceWBPaper00003469
Curator_confirmedWBPerson557
WBPhenotype:0000775Paper_evidenceWBPaper00005919
Curator_confirmedWBPerson712
RemarkThe tim-1 allele selectively disrupts meiosis.Paper_evidenceWBPaper00005919
Curator_confirmedWBPerson712
WBPhenotype:0001215Paper_evidenceWBPaper00005919
Curator_confirmedWBPerson712
RemarkHowever, under a specific temperature regime, the tim-1 allele selectively disrupts meiosis but not germline mitosis.Paper_evidenceWBPaper00005919
Curator_confirmedWBPerson712
Temperature_sensitiveHeat_sensitive25Paper_evidenceWBPaper00005919
Curator_confirmedWBPerson712
WBPhenotype:0001400Paper_evidenceWBPaper00005919
Curator_confirmedWBPerson712
RemarkIn tim-1 mutants, SCC-3 concentrations were variably reduced, and in half the gonads the residual protein was undetectable on the meiotic chromosomes.Paper_evidenceWBPaper00005919
Curator_confirmedWBPerson712
Temperature_sensitiveHeat_sensitive25Paper_evidenceWBPaper00005919
Curator_confirmedWBPerson712
WBPhenotype:0001408Paper_evidenceWBPaper00005919
Curator_confirmedWBPerson712
RemarkREC-8 was not detectable on tim-1(ts) chromosomes (data not shown) in meiotic prophase, although it was present in pre-meiotic nuclei.Paper_evidenceWBPaper00005919
Curator_confirmedWBPerson712
Temperature_sensitiveHeat_sensitive25Paper_evidenceWBPaper00005919
Curator_confirmedWBPerson712
WBPhenotype:0001814Paper_evidenceWBPaper00005919
Curator_confirmedWBPerson712
RemarkHomologous chromosomes of tim-1(ts) mutants rarely formed chiasmata. Of 142 diakinesis nuclei from wild-type germlines, all had six bivalents. Instead, 101 of 105 tim-1(ts) nuclei had 7-12 staining bodies, with an average of 10; they were mostly univalents and an occasional bivalent, indicating disrupted homologue linkage.Paper_evidenceWBPaper00005919
Curator_confirmedWBPerson712
Temperature_sensitiveHeat_sensitive25Paper_evidenceWBPaper00005919
Curator_confirmedWBPerson712
WBPhenotype:0002013Paper_evidenceWBPaper00005919
Curator_confirmedWBPerson557
RemarkHomologous chromosomes of tim-1(ts) mutants rarely formed chiasmata. Of 142 diakinesis nuclei from wild-type germlines, all had six bivalents. Instead, 101 of 105 tim-1(ts) nuclei had 7-12 staining bodies, with an average of 10; they were mostly univalents and an occasional bivalent, indicating disrupted homologue linkage.Paper_evidenceWBPaper00005919
Curator_confirmedWBPerson557
Temperature_sensitiveHeat_sensitive25Paper_evidenceWBPaper00005919
Curator_confirmedWBPerson557
WBPhenotype:0002113Paper_evidenceWBPaper00005919
Curator_confirmedWBPerson712
RemarkAlthough meiotic chromosomes of tim-1(ts) mutants reorganized into a pachytene-like arrangement just before diplotene like those of syp-1 mutants, they failed to achieve the normal lengthwise alignment typical of synapsed pachytene chromosomes. Only 17% (n = 65) of tim-1(ts) nuclei within the late pachytene region had one 5S rDNA FISH signal, in comparison with 89% (n = 93) of wild-type nuclei, indicating a severe defect in synapsis.Paper_evidenceWBPaper00005919
Curator_confirmedWBPerson712
Temperature_sensitiveHeat_sensitive25Paper_evidenceWBPaper00005919
Curator_confirmedWBPerson712
Phenotype_not_observedWBPhenotype:0001882Paper_evidenceWBPaper00005919
Curator_confirmedWBPerson712
RemarkPre-meiotic nuclei from tim-1(t1545ts) and wild-type animals grown at 25 deg C were indistinguishable.Paper_evidenceWBPaper00005919
Curator_confirmedWBPerson712
WBPhenotype:0001952Paper_evidenceWBPaper00005919
Curator_confirmedWBPerson712
RemarkTransition-zone nuclei of tim-1(ts) mutants had normal polarized organization and a similar proportion of paired and unpaired homologues to that in wild-type nuclei, on the basis of 5S rDNA FISH signals.Paper_evidenceWBPaper00005919
Curator_confirmedWBPerson712
Temperature_sensitiveHeat_sensitive25Paper_evidenceWBPaper00005919
Curator_confirmedWBPerson712
ReferenceWBPaper00018640
WBPaper00005919
WBPaper00003469
MethodAllele