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WormBase Tree Display for Variation: WBVar00241036

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Name Class

WBVar00241036EvidencePaper_evidenceWBPaper00001677
NamePublic_nameq224
Other_nameCE00237:p.Gly1043Glu
F02A9.6.1:c.3128G>A
HGVSgCHROMOSOME_III:g.9098521G>A
Sequence_detailsSMapS_parentSequenceF02A9
Flanking_sequencesctgcattgatgctagtagcacgtgaactcgaaaacatcaagtggagatggcagagcttct
Mapping_targetF02A9
Type_of_mutationSubstitutiongaPaper_evidenceWBPaper00001677
SeqStatusSequenced
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00022516
WBStrain00022530
WBStrain00022538
WBStrain00047329
LaboratoryJK
StatusLive
AffectsGeneWBGene00001609
TranscriptF02A9.6.1 (12)
InteractorWBInteraction000009194
WBInteraction000502006
WBInteraction000517590
WBInteraction000518397
IsolationMutagenEMS
GeneticsInterpolated_map_positionIII0.163976
DescriptionPhenotypeWBPhenotype:0000120Paper_evidenceWBPaper00038310
Curator_confirmedWBPerson712
RemarkTFG-1 and CAR-1 levels are significantly reduced at higher temperatures(25 deg C) compared to levels at 15 deg C, as measured by antibody staining.Paper_evidenceWBPaper00038310
Curator_confirmedWBPerson712
WBPhenotype:0000137Paper_evidenceWBPaper00031914
Curator_confirmedWBPerson712
Remarklarp-1 mRNA was reduced, but not absent.Paper_evidenceWBPaper00031914
Curator_confirmedWBPerson712
Temperature_sensitiveHeat_sensitive25Paper_evidenceWBPaper00031914
Curator_confirmedWBPerson712
Phenotype_assayTemperature25Paper_evidenceWBPaper00031914
Curator_confirmedWBPerson712
WBPhenotype:0000215Paper_evidenceWBPaper00040352
Curator_confirmedWBPerson6693
RemarkAdult hermaphrodites either had an essentially normal germline [glp-1(q224) grown at 15°C], or had virtually no germline [glp-1(q224) grown at 25°C]Paper_evidenceWBPaper00040352
Curator_confirmedWBPerson6693
Temperature_sensitiveHeat_sensitive25Paper_evidenceWBPaper00040352
Curator_confirmedWBPerson6693
WBPhenotype:0000275Paper_evidenceWBPaper00035429
Curator_confirmedWBPerson712
RemarkDNA damage accumulates at a faster rate after exposure to UVC.Paper_evidenceWBPaper00035429
Curator_confirmedWBPerson712
WBPhenotype:0000286Paper_evidenceWBPaper00001007
Curator_confirmedWBPerson2021
RemarkAlthough signs of pharyngeal differentiation is seen within the embryo, morphogenesis is defective, and a recognizable worm is not made.Paper_evidenceWBPaper00001007
Curator_confirmedWBPerson2021
PenetranceCompletePaper_evidenceWBPaper00001007
Curator_confirmedWBPerson2021
Range100100Paper_evidenceWBPaper00001007
Curator_confirmedWBPerson2021
RecessivePaper_evidenceWBPaper00001007
Curator_confirmedWBPerson2021
Variation_effectProbable_null_via_phenotypePaper_evidenceWBPaper00001007
Curator_confirmedWBPerson2021
EQ_annotationsLife_stageWBls:0000003PATO:0000460Paper_evidenceWBPaper00001007
Curator_confirmedWBPerson2021
Temperature_sensitiveHeat_sensitive25Paper_evidenceWBPaper00001007
Curator_confirmedWBPerson2021
MaternalStrictly_maternalPaper_evidenceWBPaper00001007
Curator_confirmedWBPerson2021
WBPhenotype:0000351Paper_evidenceWBPaper00060633
Curator_confirmedWBPerson37673
Variation_effectLoss_of_function_undetermined_extentPaper_evidenceWBPaper00060633
Curator_confirmedWBPerson37673
Temperature_sensitiveHeat_sensitivePaper_evidenceWBPaper00060633
Curator_confirmedWBPerson37673
Phenotype_assay (2)
WBPhenotype:0000621Paper_evidenceWBPaper00001423
Curator_confirmedWBPerson2021
RemarkThere is no detectable excretory cellPaper_evidenceWBPaper00001423
Curator_confirmedWBPerson2021
PenetranceLowPaper_evidenceWBPaper00001423
Curator_confirmedWBPerson2021
Range11Paper_evidenceWBPaper00001423
Curator_confirmedWBPerson2021
EQ_annotationsAnatomy_termWBbt:0005812PATO:0000460Paper_evidenceWBPaper00001423
Curator_confirmedWBPerson2021
WBbt:0005777PATO:0000460Paper_evidenceWBPaper00001423
Curator_confirmedWBPerson2021
Life_stageWBls:0000024PATO:0000460Paper_evidenceWBPaper00001423
Curator_confirmedWBPerson2021
Temperature_sensitiveHeat_sensitivePaper_evidenceWBPaper00001423
Curator_confirmedWBPerson2021
Phenotype_assayTreatmentOnly observed when progeny from a homozygous glp-1(g224ts) hermaphrodite are shifted to the restrictive temperature during early embryogenesisPaper_evidenceWBPaper00001423
Curator_confirmedWBPerson2021
WBPhenotype:0000646Paper_evidenceWBPaper00029334
Curator_confirmedWBPerson712
RemarkAlthough dead nematodes were not observed, the UVC-exposed nematodes were sluggish between 24 and 72 hours after exposure.Paper_evidenceWBPaper00029334
Curator_confirmedWBPerson712
Phenotype_assayTreatmentWe exposed 1-day-old glp-1 adultsto 400 J/m2 UVC, allowed them to recover for 3 hours to 3 days, and then analyzed lesion frequencies in the polymerase epsilon target (Figure 5).Paper_evidenceWBPaper00029334
Curator_confirmedWBPerson712
WBPhenotype:0000684Paper_evidenceWBPaper00001007
Curator_confirmedWBPerson2021
RemarkOnly 6-8 germ cells are produced in glp-1 mutants. All these germ cells differentiate into spermPaper_evidenceWBPaper00001007
Curator_confirmedWBPerson2021
PenetranceCompletePaper_evidenceWBPaper00001007
Curator_confirmedWBPerson2021
Range100100Paper_evidenceWBPaper00001007
Curator_confirmedWBPerson2021
RecessivePaper_evidenceWBPaper00001007
Curator_confirmedWBPerson2021
Variation_effectProbable_null_via_phenotypePaper_evidenceWBPaper00001007
Curator_confirmedWBPerson2021
EQ_annotationsLife_stageWBls:0000056PATO:0000460Paper_evidenceWBPaper00001007
Curator_confirmedWBPerson2021
WBls:0000057PATO:0000460Paper_evidenceWBPaper00001007
Curator_confirmedWBPerson2021
Temperature_sensitiveHeat_sensitive25Paper_evidenceWBPaper00001007
Curator_confirmedWBPerson2021
WBPhenotype:0000688Paper_evidenceWBPaper00060633
Curator_confirmedWBPerson37673
Variation_effectLoss_of_function_undetermined_extentPaper_evidenceWBPaper00060633
Curator_confirmedWBPerson37673
Temperature_sensitiveHeat_sensitivePaper_evidenceWBPaper00060633
Curator_confirmedWBPerson37673
Phenotype_assay (2)
WBPhenotype:0000717Paper_evidenceWBPaper00035429
Curator_confirmedWBPerson712
RemarkGlobal gene expression was highly divergent in the glp-1 strain from either wild-type or xpa-1, in both conditions.Paper_evidenceWBPaper00035429
Curator_confirmedWBPerson712
WBPhenotype:0000823Paper_evidenceWBPaper00035429
Curator_confirmedWBPerson712
RemarkAnimals lack germ cells.Paper_evidenceWBPaper00035429
Curator_confirmedWBPerson712
WBPhenotype:0000867Paper_evidenceWBPaper00001007
Curator_confirmedWBPerson2021
PenetranceCompletePaper_evidenceWBPaper00001007
Curator_confirmedWBPerson2021
Range100100Paper_evidenceWBPaper00001007
Curator_confirmedWBPerson2021
RecessivePaper_evidenceWBPaper00001007
Curator_confirmedWBPerson2021
Variation_effectProbable_null_via_phenotypePaper_evidenceWBPaper00001007
Curator_confirmedWBPerson2021
EQ_annotationsLife_stageWBls:0000003PATO:0000460Paper_evidenceWBPaper00001007
Curator_confirmedWBPerson2021
Temperature_sensitiveHeat_sensitive25Paper_evidenceWBPaper00001007
Curator_confirmedWBPerson2021
MaternalStrictly_maternalPaper_evidenceWBPaper00001007
Curator_confirmedWBPerson2021
WBPhenotype:0001014Paper_evidenceWBPaper00035490
Curator_confirmedWBPerson2021
Remarkglp-1 mutants extended nematode survival in the presence of P. aeruginosa and S. marcescensPaper_evidenceWBPaper00035490
Curator_confirmedWBPerson2021
WBPhenotype:0001098Paper_evidenceWBPaper00001423
Curator_confirmedWBPerson2021
PenetranceLowPaper_evidenceWBPaper00001423
Curator_confirmedWBPerson2021
Range11Paper_evidenceWBPaper00001423
Curator_confirmedWBPerson2021
EQ_annotationsAnatomy_termWBbt:0005773PATO:0000460Paper_evidenceWBPaper00001423
Curator_confirmedWBPerson2021
Life_stageWBls:0000024PATO:0000460Paper_evidenceWBPaper00001423
Curator_confirmedWBPerson2021
Temperature_sensitiveHeat_sensitivePaper_evidenceWBPaper00001423
Curator_confirmedWBPerson2021
Phenotype_assayTreatmentOnly observed when progeny from a homozygous glp-1(g224ts) hermaphrodite are shifted to the restrictive temperature during early embryogenesisPaper_evidenceWBPaper00001423
Curator_confirmedWBPerson2021
WBPhenotype:0001099Paper_evidenceWBPaper00001423
Curator_confirmedWBPerson2021
PenetranceLowPaper_evidenceWBPaper00001423
Curator_confirmedWBPerson2021
Range11Paper_evidenceWBPaper00001423
Curator_confirmedWBPerson2021
EQ_annotationsLife_stageWBls:0000024PATO:0000460Paper_evidenceWBPaper00001423
Curator_confirmedWBPerson2021
Temperature_sensitiveHeat_sensitivePaper_evidenceWBPaper00001423
Curator_confirmedWBPerson2021
Phenotype_assayTreatmentOnly observed when progeny from a homozygous glp-1(g224ts) hermaphrodite are shifted to the restrictive temperature during early embryogenesisPaper_evidenceWBPaper00001423
Curator_confirmedWBPerson2021
WBPhenotype:0001410Paper_evidenceWBPaper00033205
Curator_confirmedWBPerson2021
RemarkRNP-8L protein was absent from glp-1 mutants with no germlinePaper_evidenceWBPaper00033205
Curator_confirmedWBPerson2021
Temperature_sensitiveHeat_sensitive25CPaper_evidenceWBPaper00033205
Curator_confirmedWBPerson2021
WBPhenotype:0002120Paper_evidenceWBPaper00029334
Curator_confirmedWBPerson712
RemarkNo statistically significant removal of mitochondrial lesions was observed (Figure 3b). In N2 the lesion frequency decreased significantly (Figure 3a) in both the nuclear and mitochondrial genomes at 6 and 24 hours.Paper_evidenceWBPaper00029334
Curator_confirmedWBPerson712
Phenotype_assay (2)
WBPhenotype:0002129Paper_evidenceWBPaper00041209
Curator_confirmedWBPerson712
RemarkPost-mitotic young adult glp-1 nematodes raised at 25C were exposed to 0-100 J/m2UVC and both nuclear and mtDNA damage was analyzed at 0, 24, 48 and 72 h post exposure via a highly sensitive QPCR assay that does not require differential extraction of nuclear and mtDNA (55,56,69). Over 72 h, 30-40% removal of mtDNA damage was observed at 50 and 100 J/m2 (Figure 1a). nDNA damage was repaired to control level by 72 h (Figure 1b). Poisson distribution of lesions and a similar mtDNA copy number per cell, even at 50 and 100 J/m2 35 and 20%, respectively, of mtDNA genomes would be predicted to remain undamaged. Thus, in principle, the reduction in mtDNA damage observed here could result from dilution of damage rather than removal; however, mtDNA copy number did not increase during the recovery period at any UV dose as measured b real-time PCR (Figure 1c). In fact, there was a significant decrease in copy number across treatments by 48 h post exposure (including controls; Figure 1d); this decrease was not altered by the level of damage (P=0.3246 for treatment time interaction).Paper_evidenceWBPaper00041209
Curator_confirmedWBPerson712
WBPhenotype:0002430Paper_evidenceWBPaper00041209
Curator_confirmedWBPerson712
RemarkPost-mitotic young adult glp-1 nematodes raised at 25C were exposed to 0-100 J/m2 UVC and both nuclear and mtDNA damage was analyzed at 0, 24, 48 and 72 h post exposure via a highly sensitive QPCR assay that does not require differential extraction of nuclear and mtDNA (55,56,69). Over 72 h, 30-40% removal of mtDNA damage was observed at 50 and 100 J/m2 (Figure 1a). nDNA damage was repaired to control level by 72 h (Figure 1b). Poisson distribution of lesions and a similar mtDNA copy number per cell, even at 50 and 100 J/m2 35 and 20%, respectively, of mtDNA genomes would be predicted to remain undamaged. Thus, in principle, the reduction in mtDNA damage observed here could result from dilution of damage rather than removal; however, mtDNA copy number did not increase during the recovery period at any UV dose as measured b real-time PCR (Figure 1c). In fact, there was a significant decrease in copy number across treatments by 48 h post exposure (including controls; Figure 1d); this decrease wa snot altered by the level of damage (P=0.3246 for treatment time interaction).Paper_evidenceWBPaper00041209
Curator_confirmedWBPerson712
Phenotype_not_observedWBPhenotype:0000412Paper_evidenceWBPaper00038400
Curator_confirmedWBPerson712
RemarkAnimals responded robustly to octanol.Paper_evidenceWBPaper00038400
Curator_confirmedWBPerson712
Variation_effectHypomorph_reduction_of_functionPaper_evidenceWBPaper00038400
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00001966Paper_evidenceWBPaper00038400
Curator_confirmedWBPerson712
Temperature_sensitiveHeat_sensitivePaper_evidenceWBPaper00038400
Curator_confirmedWBPerson712
WBPhenotype:0000739Paper_evidenceWBPaper00029334
Curator_confirmedWBPerson712
RemarkDifferent life stages of N2 or glp-1 nematodes exposed to 0, 100, or 200 J/m2 UVC exhibited marked differences in susceptibility to induction of DNA damage (Figure 2), with starved L1 larvae the most and 1-day-old N2 adults the least susceptible. No differences were observed in terms of damage to the nuclear (DNA polymerase epsilon target) and mitochondrial genome at any life stage. We also compared eggs isolated by bleach-sodium hydroxide treatment but not exposed to UVC, with unexposed eggs isolated by wash-off (eggs already laid), to test whether the bleach-sodium hydroxide treatment had a detectable effect on DNA integrity. No difference was detected (data not shown).Paper_evidenceWBPaper00029334
Curator_confirmedWBPerson712
Phenotype_assayStrainWBStrain00022538Paper_evidenceWBPaper00029334
Curator_confirmedWBPerson712
WBPhenotype:0000977Paper_evidenceWBPaper00001007
Curator_confirmedWBPerson2021
RecessivePaper_evidenceWBPaper00001007
Curator_confirmedWBPerson2021
Variation_effectProbable_null_via_phenotypePaper_evidenceWBPaper00001007
Curator_confirmedWBPerson2021
EQ_annotationsLife_stageWBls:0000041PATO:0000460Paper_evidenceWBPaper00001007
Curator_confirmedWBPerson2021
WBPhenotype:0001661Paper_evidenceWBPaper00003760
Curator_confirmedWBPerson2021
RemarkAsymmetric expression in AWC was normalPaper_evidenceWBPaper00003760
Curator_confirmedWBPerson2021
Temperature_sensitiveHeat_sensitivePaper_evidenceWBPaper00003760
Curator_confirmedWBPerson2021
WBPhenotype:0001938Paper_evidenceWBPaper00029334
Curator_confirmedWBPerson712
RemarkNo detectable difference in repair was observed between the N2 and glp-1 L1 larvae (Figure 4). Removal of nuclear lesions was apparent in glp-1 adults. In N2 the lesion frequency decreased significantly (Figure 3a) in both the nuclear and mitochondrial genomes at 6 and 24 hours.Paper_evidenceWBPaper00029334
Curator_confirmedWBPerson712
Phenotype_assay (2)
WBPhenotype:0002430Paper_evidenceWBPaper00029334
Curator_confirmedWBPerson712
RemarkDifferent life stages of N2 or glp-1 nematodes exposed to 0, 100, or 200 J/m2 UVC exhibited marked differences in susceptibility to induction of DNA damage (Figure 2), with starved L1 larvae the most and 1-day-old N2 adults the least susceptible. No differences were observed in terms of damage to the nuclear (DNA polymerase epsilon target) and mitochondrial genome at any life stage. We also compared eggs isolated by bleach-sodium hydroxide treatment but not exposed to UVC, with unexposed eggs isolated by wash-off (eggs already laid), to test whether the bleach-sodium hydroxide treatment had a detectable effect on DNA integrity. No difference was detected (data not shown).Paper_evidenceWBPaper00029334
Curator_confirmedWBPerson712
Phenotype_assayStrainWBStrain00022538Paper_evidenceWBPaper00029334
Curator_confirmedWBPerson712
Reference (16)
MethodSubstitution_allele