WormBase Tree Display for Variation: WBVar00144965

WBVar00144965 Evidence: Paper_evidence: WBPaper00026735
  Name: Public_name: e2740
  Sequence_details: SeqStatus: Pending_curation
  Variation_type: Allele
  Origin: Species: Caenorhabditis elegans
    Strain: WBStrain00004664
    Laboratory: CB
    Status: Live
  Affects: Gene: WBGene00010442
  Isolation: Mutagen: mut-7
  Description: Phenotype: WBPhenotype:0001014 Person_evidence: WBPerson261
        Curator_confirmed: WBPerson48
        Remark: Resistant to infection by M. nematophilum. Person_evidence: WBPerson261
            Curator_confirmed: WBPerson48
        Penetrance: Complete: Person_evidence: WBPerson261
              Curator_confirmed: WBPerson48
        Recessive: Person_evidence: WBPerson261
          Curator_confirmed: WBPerson48
        Variation_effect: Loss_of_function_undetermined_extent: Person_evidence: WBPerson261
            Curator_confirmed: WBPerson48
      WBPhenotype:0001411 Paper_evidence: WBPaper00026735
        Curator_confirmed: WBPerson48
        Remark: Based on lectin binding properties to the cuticle Paper_evidence: WBPaper00026735
            Curator_confirmed: WBPerson48
        Phenotype_assay: Treatment: Tested the following fluorescein or rhodamine-conjugated lectins: WGA, SBA, TPA, PEA,, CON, LEA. Paper_evidence: WBPaper00026735
              Curator_confirmed: WBPerson48
      WBPhenotype:0001413 Paper_evidence: WBPaper00026735
          WBPaper00037686
        Curator_confirmed: WBPerson48
          WBPerson712
        Remark: Slight tail swelling observed when grown with M. nematophilum. Paper_evidence: WBPaper00026735
            Curator_confirmed: WBPerson48
          Phenotype is perceptively weaker than br5, and significant rectal accumulation of the M. nematophilum pathogen can be seen in some infected worms. Paper_evidence: WBPaper00037686
            Curator_confirmed: WBPerson712
        Penetrance: Incomplete: variable Paper_evidence: WBPaper00026735
              Curator_confirmed: WBPerson48
      WBPhenotype:0001415 Paper_evidence: WBPaper00026735
        Curator_confirmed: WBPerson48
        Remark: Based on vital dye staining for bacteria to reveal M. nematophilum; less rectal staining, indicating less adherence in the usual region, but some abnormal staining in males relative to wild type. Paper_evidence: WBPaper00026735
            Curator_confirmed: WBPerson48
      WBPhenotype:0001420 Paper_evidence: WBPaper00029153
          WBPaper00031925
        Curator_confirmed: WBPerson712
        Remark: Leaky Bah - biofilm formed on the side of the head and not over the mouth, allowing normal feeding and growth. Paper_evidence: WBPaper00029153
            Curator_confirmed: WBPerson712
          Animals are completely resistant to attachment of biofilm produced by Xenorhabdus nematophila. Paper_evidence: WBPaper00031925
            Curator_confirmed: WBPerson712
        Penetrance: Incomplete: Phenotype is somewhat leaky. Paper_evidence: WBPaper00029153
              Curator_confirmed: WBPerson712
          Complete: Paper_evidence: WBPaper00031925
              Curator_confirmed: WBPerson712
        Phenotype_assay: Treatment: Biofilm formation on adult worms was assessed for worms incubated 24-48 hours on the bacterial lawn. Alternatively, gravid adults were allowed to lay eggs on bacterial lawns for several hours, then removed and larvae were assessed for biofilm attachment the next day. Paper_evidence: WBPaper00031925
              Curator_confirmed: WBPerson712
      WBPhenotype:0002109 Paper_evidence: WBPaper00062695
        Curator_confirmed: WBPerson712
        Remark: We used a bus-12(e2740) that has a fragile cuticle (Darby et al. 2007) and found DEC had similar effects compared to the N2 (Fig 1C and D). The IC50 for bus-12 was 0.50.04mM, indistinguishable from N2, indicating DEC passes through the cuticle, and its lack of potency may be due to reduced effect on its target receptor. Paper_evidence: WBPaper00062695
            Curator_confirmed: WBPerson712
        Affected_by: Molecule: WBMol:00003038 Paper_evidence: WBPaper00062695
              Curator_confirmed: WBPerson712
            WBMol:00007968 Paper_evidence: WBPaper00062695
              Curator_confirmed: WBPerson712
    Phenotype_not_observed: WBPhenotype:0001209 Paper_evidence: WBPaper00029153
        Curator_confirmed: WBPerson712
        Penetrance: Incomplete: Phenotype is somewhat leaky. Paper_evidence: WBPaper00029153
              Curator_confirmed: WBPerson712
      WBPhenotype:0001212 Paper_evidence: WBPaper00026735
        Curator_confirmed: WBPerson48
        Remark: Based on a comparison of bleach sensitivity with wild type worms. Paper_evidence: WBPaper00026735
            Curator_confirmed: WBPerson48
  Reference: WBPaper00026735
    WBPaper00031925
    WBPaper00029153
    WBPaper00037686
    WBPaper00062695
  Remark: mut-7 induced
  Method: Allele