WormBase Tree Display for Variation: WBVar00144578

WBVar00144578 Name: Public_name: e2117
  Sequence_details: SeqStatus: Pending_curation
  Variation_type: Allele
  Origin: Species: Caenorhabditis elegans
    Strain: WBStrain00004541
    Laboratory: CB
    Status: Live
  Affects: Gene: WBGene00005018
    Interactor: WBInteraction000537319
  Description: Phenotype: WBPhenotype:0000019 Paper_evidence: WBPaper00003348
        Curator_confirmed: WBPerson712
        Remark: Mean pumping rate is 160 pumps/min compared to 275 pumps/min. Paper_evidence: WBPaper00003348
            Curator_confirmed: WBPerson712
      WBPhenotype:0000057 Person_evidence: WBPerson261
        Curator_confirmed: WBPerson712
        Remark: early larval lethal at 25C Person_evidence: WBPerson261
            Curator_confirmed: WBPerson712
        Temperature_sensitive: Heat_sensitive: 25 Person_evidence: WBPerson261
              Curator_confirmed: WBPerson712
      WBPhenotype:0000583 Paper_evidence: WBPaper00005747
        Person_evidence: WBPerson261
        Curator_confirmed: WBPerson712
          WBPerson2987
        Remark: extreme dumpy at 25C Person_evidence: WBPerson261
            Curator_confirmed: WBPerson712
          Authors report "extreme dpy", temperature sensitive (Table 1) Paper_evidence: WBPaper00005747
            Curator_confirmed: WBPerson2987
        Temperature_sensitive: Heat_sensitive: 25 Person_evidence: WBPerson261
              Curator_confirmed: WBPerson712
        Phenotype_assay: Genotype: kaIs12 [col-19::gfp] Paper_evidence: WBPaper00005747
              Curator_confirmed: WBPerson2987
      WBPhenotype:0000749 Paper_evidence: WBPaper00000927
        Curator_confirmed: WBPerson712
        Remark: Embryos retract after elongation. Paper_evidence: WBPaper00000927
            Curator_confirmed: WBPerson712
        Temperature_sensitive: Heat_sensitive: 25 Paper_evidence: WBPaper00000927
              Curator_confirmed: WBPerson712
        Phenotype_assay: Temperature: 15C, 25C Paper_evidence: WBPaper00000927
              Curator_confirmed: WBPerson712
      WBPhenotype:0000843 Paper_evidence: WBPaper00003348
        Curator_confirmed: WBPerson712
      WBPhenotype:0000948 Paper_evidence: WBPaper00000927
        Curator_confirmed: WBPerson712
        Remark: The striated layer of the cuticle is missing in retracted embryos. Paper_evidence: WBPaper00000927
            Curator_confirmed: WBPerson712
        Temperature_sensitive: Heat_sensitive: 25 Paper_evidence: WBPaper00000927
              Curator_confirmed: WBPerson712
        Phenotype_assay: Temperature: 15C, 25C Paper_evidence: WBPaper00000927
              Curator_confirmed: WBPerson712
      WBPhenotype:0000961 Paper_evidence: WBPaper00005747
        Curator_confirmed: WBPerson2987
        Remark: Table 1 Paper_evidence: WBPaper00005747
            Curator_confirmed: WBPerson2987
        Phenotype_assay: Genotype: kaIs12 [col-19::gfp] Paper_evidence: WBPaper00005747
              Curator_confirmed: WBPerson2987
      WBPhenotype:0001412 Paper_evidence: WBPaper00005747
        Curator_confirmed: WBPerson2987
        Remark: Authors report "abnormal branched ala" (Table 1) Paper_evidence: WBPaper00005747
            Curator_confirmed: WBPerson2987
        Phenotype_assay: Genotype: kaIs12 [col-19::gfp] Paper_evidence: WBPaper00005747
              Curator_confirmed: WBPerson2987
      WBPhenotype:0001517 Paper_evidence: WBPaper00005747
        Curator_confirmed: WBPerson2987
        Remark: Authors report "severe disruption" (Table 1) Paper_evidence: WBPaper00005747
            Curator_confirmed: WBPerson2987
        Phenotype_assay: Genotype: kaIs12 [col-19::gfp] Paper_evidence: WBPaper00005747
              Curator_confirmed: WBPerson2987
    Phenotype_not_observed: WBPhenotype:0001732 Paper_evidence: WBPaper00032033
        Curator_confirmed: WBPerson712
        Remark: Animals exhibited the same pH survival profile as the wild-type strain over a range of pH 3 to pH 10. Paper_evidence: WBPaper00032033
            Curator_confirmed: WBPerson712
        Phenotype_assay: Treatment: Mixed or synchronised populations of nematodes were exposed to 500ul M9 buffer (100 mM phosphate, 85 mM NaCl, 1 mM MgSO4 buffered at the appropriate pH by varying KH2PO4 and Na2HPO4 concentrations accordingly) at varying pHs in a 24-well plate format for 4 h (200 nematodes/well). Nematodes were scored as viable by the presence of touch response and pharyngeal pumping. Paper_evidence: WBPaper00032033
              Curator_confirmed: WBPerson712
  Reference: WBPaper00005704
    WBPaper00005747
    WBPaper00032033
    WBPaper00003348
    WBPaper00000927
    WBPaper00013885
  Method: Allele