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WormBase Tree Display for Variation: WBVar00092700

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Name Class

WBVar00092700EvidencePaper_evidenceWBPaper00033444
NamePublic_nameok1489
Other_nameC55B7.4b.1:c.162_667del
C55B7.4b.2:c.162_667del
CE32840:p.Glu55ThrfsTer3
C55B7.4a.1:c.441_946del
CE09015:p.Glu148ThrfsTer3
HGVSgCHROMOSOME_I:g.6498246_6498845del
Sequence_detailsSMapS_parentSequenceC55B7
Flanking_sequencestgtatcaatgattatagattatggaacagagacttattgattttcaggttatttttgaaa
Mapping_targetC55B7
Type_of_mutationDeletion
PCR_productok1489_external
ok1489_internal
SeqStatusSequenced
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00036246
LaboratoryRB
PersonWBPerson46
KO_consortium_allele
StatusLive
AffectsGeneWBGene00016943
TranscriptC55B7.4b.1 (11)
C55B7.4a.1 (11)
C55B7.4b.2 (11)
InteractorWBInteraction000566982
IsolationMutagenEMS
DescriptionPhenotypeWBPhenotype:0000050Paper_evidenceWBPaper00049812
Curator_confirmedWBPerson1997
Remarkon very low vitamin B12 diets (Figure 2C)Paper_evidenceWBPaper00049812
Curator_confirmedWBPerson1997
PenetranceCompletePaper_evidenceWBPaper00049812
Curator_confirmedWBPerson1997
RecessivePaper_evidenceWBPaper00049812
Curator_confirmedWBPerson1997
Phenotype_assayControl_strainWBStrain00000001Paper_evidenceWBPaper00049812
Curator_confirmedWBPerson1997
TreatmentWorms fed a diet very low in vitamin B12Paper_evidenceWBPaper00049812
Curator_confirmedWBPerson1997
WBPhenotype:0000061Paper_evidenceWBPaper00059839
Curator_confirmedWBPerson42723
Remarkextended lifespan upon exposure to S. maltophilia JV3Paper_evidenceWBPaper00059839
Curator_confirmedWBPerson42723
Variation_effectNullPaper_evidenceWBPaper00059839
Curator_confirmedWBPerson42723
Phenotype_assayControl_strainWBStrain00000001Paper_evidenceWBPaper00059839
Curator_confirmedWBPerson42723
WBPhenotype:0000136Paper_evidenceWBPaper00042204
Curator_confirmedWBPerson2987
RemarkOn a Comamonas DA1877 diet, acdh-1(ok1489) animals exhibited increased mRNA levels of endogenous acdh-2, nhr-68, and cth-1Paper_evidenceWBPaper00042204
Curator_confirmedWBPerson2987
On an E. coli OP50 diet, acdh-1(ok1489) animals exhibited increased mRNA levels of endogenous acdh-2, ech-6, F09F7.4 and cth-1Paper_evidenceWBPaper00042204
Curator_confirmedWBPerson2987
Phenotype_assayTreatmentWorms fed on a Comamonas DA1877 dietPaper_evidenceWBPaper00042204
Curator_confirmedWBPerson2987
Worms fed on a E. coli OP50 dietPaper_evidenceWBPaper00042204
Curator_confirmedWBPerson2987
WBPhenotype:0000519Paper_evidenceWBPaper00033444
Curator_confirmedWBPerson2021
RemarkMutant strains showed minor differences in absolute fitness compared to wild type when exposed to different bacterial environments such as E. coli, M. luteus, and Pseudomonas sp.and B. megateriumPaper_evidenceWBPaper00033444
Curator_confirmedWBPerson2021
Variation_effectNullPaper_evidenceWBPaper00033444
Curator_confirmedWBPerson2021
WBPhenotype:0001171Paper_evidenceWBPaper00059839
Curator_confirmedWBPerson42723
Remarkshortened lifespan upon exposure to E. coli OP50, S. maltophilia K279a and JCMSPaper_evidenceWBPaper00059839
Curator_confirmedWBPerson42723
Variation_effectNullPaper_evidenceWBPaper00059839
Curator_confirmedWBPerson42723
Phenotype_assayControl_strainWBStrain00000001Paper_evidenceWBPaper00059839
Curator_confirmedWBPerson42723
WBPhenotype:0001236Paper_evidenceWBPaper00042204
Curator_confirmedWBPerson2987
Remark"Interestingly, acdh-1 itself was found as a repressor in the RNAi screen (Table S2). We obtained an acdh-1 deletion mutant and introduced it into the Pacdh-1::GFP dietary sensor strain. We observed higher GFP levels in the acdh-1 mutant relative to the wild-type dietary sensor strain when the animals were fed E. Coli OP50 or HT115 diets but observed less of an increase on a Comamonas DA1877 diet." (Figure 4A)Paper_evidenceWBPaper00042204
Curator_confirmedWBPerson2987
Phenotype_assayGenotypePacdh-1::GFPPaper_evidenceWBPaper00042204
Curator_confirmedWBPerson2987
WBPhenotype:0001918Paper_evidenceWBPaper00044854
Curator_confirmedWBPerson2987
Remark"We tested the sensitivity of Dacdh-1 mutants to propionic acid and found that they are much more sensitive than wild-type animals (Figure 6B). Unlike Dpcca-1 mutants, however, Dacdh-1 mutants survived better on propionic acid when vitamin B12 was supplemented to the diet."Paper_evidenceWBPaper00044854
Curator_confirmedWBPerson2987
Affected_byMoleculeWBMol:00004332Paper_evidenceWBPaper00044854
Curator_confirmedWBPerson2987
Phenotype_not_observedWBPhenotype:0000679Paper_evidenceWBPaper00042204
Curator_confirmedWBPerson2987
Remark"To determine whether known nutrient sensing pathways such as TOR or the insulin signaling pathway are involved in upregulating acdh-1 in response to metabolic network perturbations, we performed RNAi on a panel of genes from these pathways in metabolic mutants harboring the Pacdh-1::GFP transgene. Knockdown of daf-2 or daf-16 had no effect on GFP expression in any of the mutants (Figure 6A). We also crossed the Dmetr-1 and Dacdh-1 metabolic mutations into a transgenic strain expressing a DAF-16::GFP fusion protein to determine whether these metabolic mutants affected DAF-16 nuclear localization and found that neither had any effect (data not shown)."Paper_evidenceWBPaper00042204
Curator_confirmedWBPerson2987
Phenotype_assayGenotypeDAF-16::GFPPaper_evidenceWBPaper00042204
Curator_confirmedWBPerson2987
ReferenceWBPaper00042204
WBPaper00033444
WBPaper00044854
WBPaper00049812
WBPaper00059839
WBPaper00065272
RemarkSequenced by the C. elegans Gene Knockout ConsortiumPaper_evidenceWBPaper00041807
MethodKO_consortium_allele