WormBase Tree Display for Variation: WBVar00091375

WBVar00091375 Evidence: Paper_evidence: WBPaper00026928
  Name: Public_name: nr2009
  Sequence_details: Mapping_target: ZK632
    Type_of_mutation: Deletion
    SeqStatus: Pending_curation
  Variation_type: Allele
  Origin: Species: Caenorhabditis elegans
    Strain: WBStrain00029111
    Laboratory: NS
    Status: Live
  Affects: Gene: WBGene00000567
    Interactor: WBInteraction000571515
  Genetics: Mapping_data: In_multi_point: 4231
  Description: Phenotype: WBPhenotype:0000031 Paper_evidence: WBPaper00026928
        Curator_confirmed: WBPerson2021
        Remark: Deletion mutants grew more slowly than wild type Paper_evidence: WBPaper00026928
            Curator_confirmed: WBPerson2021
        Variation_effect: Null: Paper_evidence: WBPaper00026928
            Curator_confirmed: WBPerson2021
      WBPhenotype:0000057 Paper_evidence: WBPaper00026928
        Curator_confirmed: WBPerson2021
        Remark: Mutant embryos hatch but then die with deformed intestines and tails at 25C Paper_evidence: WBPaper00026928
            Curator_confirmed: WBPerson2021
        Variation_effect: Null: Paper_evidence: WBPaper00026928
            Curator_confirmed: WBPerson2021
        EQ_annotations: Life_stage: WBls:0000023 PATO:0000460 Paper_evidence: WBPaper00026928
                Curator_confirmed: WBPerson2021
        Temperature_sensitive: Heat_sensitive: 25C Paper_evidence: WBPaper00026928
              Curator_confirmed: WBPerson2021
        Phenotype_assay: Temperature: 25C Paper_evidence: WBPaper00026928
              Curator_confirmed: WBPerson2021
      WBPhenotype:0000059 Paper_evidence: WBPaper00026928
        Curator_confirmed: WBPerson2021
        Remark: Arrested at the early L2 stage at 25C Paper_evidence: WBPaper00026928
            Curator_confirmed: WBPerson2021
        Variation_effect: Null: Paper_evidence: WBPaper00026928
            Curator_confirmed: WBPerson2021
        EQ_annotations: Life_stage: WBls:0000023 PATO:0000460 Paper_evidence: WBPaper00026928
                Curator_confirmed: WBPerson2021
        Temperature_sensitive: Heat_sensitive: 25C Paper_evidence: WBPaper00026928
              Curator_confirmed: WBPerson2021
        Phenotype_assay: Temperature: 25C Paper_evidence: WBPaper00026928
              Curator_confirmed: WBPerson2021
      WBPhenotype:0000069 Paper_evidence: WBPaper00026928
        Curator_confirmed: WBPerson2021
        Remark: The cnx-1(nr2009) mutant worms produced approximately 50% of wild-type progeny at 20 C and only 30% at 25 C Paper_evidence: WBPaper00026928
            Curator_confirmed: WBPerson2021
        Variation_effect: Null: Paper_evidence: WBPaper00026928
            Curator_confirmed: WBPerson2021
        Temperature_sensitive: Heat_sensitive: 25C Paper_evidence: WBPaper00026928
              Curator_confirmed: WBPerson2021
        Phenotype_assay: Temperature: 20C, 25C Paper_evidence: WBPaper00026928
              Curator_confirmed: WBPerson2021
      WBPhenotype:0000141 Paper_evidence: WBPaper00026928
        Curator_confirmed: WBPerson2021
        Remark: When mutant worms were shifted from 20 to 25 C, the viability of their progeny was significantly decreased to about 50% compared to wild-type Paper_evidence: WBPaper00026928
            Curator_confirmed: WBPerson2021
        Variation_effect: Null: Paper_evidence: WBPaper00026928
            Curator_confirmed: WBPerson2021
        Temperature_sensitive: Heat_sensitive: 25C Paper_evidence: WBPaper00026928
              Curator_confirmed: WBPerson2021
        Phenotype_assay: Temperature: 20C, 25C Paper_evidence: WBPaper00026928
              Curator_confirmed: WBPerson2021
      WBPhenotype:0000154 Paper_evidence: WBPaper00026928
        Curator_confirmed: WBPerson2021
        Remark: cnx-1(nr2009) mutant worms showed a significantly decreased brood size at 20 C (149 17) and 25 C (54 27) Paper_evidence: WBPaper00026928
            Curator_confirmed: WBPerson2021
        Variation_effect: Null: Paper_evidence: WBPaper00026928
            Curator_confirmed: WBPerson2021
        Temperature_sensitive: Heat_sensitive: 25C Paper_evidence: WBPaper00026928
              Curator_confirmed: WBPerson2021
        Phenotype_assay: Temperature: 20C, 25C Paper_evidence: WBPaper00026928
              Curator_confirmed: WBPerson2021
      WBPhenotype:0000286 Paper_evidence: WBPaper00026928
        Curator_confirmed: WBPerson2021
        Remark: Some of the embryos are disorganized and undifferentiated at 25C Paper_evidence: WBPaper00026928
            Curator_confirmed: WBPerson2021
        Variation_effect: Null: Paper_evidence: WBPaper00026928
            Curator_confirmed: WBPerson2021
        EQ_annotations: Life_stage: WBls:0000003 PATO:0000460 Paper_evidence: WBPaper00026928
                Curator_confirmed: WBPerson2021
        Temperature_sensitive: Heat_sensitive: 25C Paper_evidence: WBPaper00026928
              Curator_confirmed: WBPerson2021
        Phenotype_assay: Temperature: 25C Paper_evidence: WBPaper00026928
              Curator_confirmed: WBPerson2021
      WBPhenotype:0000324 Paper_evidence: WBPaper00026928
        Curator_confirmed: WBPerson2021
        Remark: Deletion mutants were slightly shorter in body-length than wild type Paper_evidence: WBPaper00026928
            Curator_confirmed: WBPerson2021
        Variation_effect: Null: Paper_evidence: WBPaper00026928
            Curator_confirmed: WBPerson2021
      WBPhenotype:0000750 Paper_evidence: WBPaper00026928
        Curator_confirmed: WBPerson2021
        Remark: Severe defects of the whole body, especially in the intestine and tail regions at 25C Paper_evidence: WBPaper00026928
            Curator_confirmed: WBPerson2021
        Variation_effect: Null: Paper_evidence: WBPaper00026928
            Curator_confirmed: WBPerson2021
        EQ_annotations: Life_stage: WBls:0000023 PATO:0000460 Paper_evidence: WBPaper00026928
                Curator_confirmed: WBPerson2021
        Temperature_sensitive: Heat_sensitive: 25C Paper_evidence: WBPaper00026928
              Curator_confirmed: WBPerson2021
        Phenotype_assay: Temperature: 25C Paper_evidence: WBPaper00026928
              Curator_confirmed: WBPerson2021
      WBPhenotype:0000812 Paper_evidence: WBPaper00026928
        Curator_confirmed: WBPerson2021
        Remark: Sperm and oocytes produced by cnx-1(nr2009) were less fertile than those of wild type Paper_evidence: WBPaper00026928
            Curator_confirmed: WBPerson2021
        Variation_effect: Null: Paper_evidence: WBPaper00026928
            Curator_confirmed: WBPerson2021
      WBPhenotype:0000867 Paper_evidence: WBPaper00026928
        Curator_confirmed: WBPerson2021
        Remark: cnx-1 mutants show characteristic embryonic arrest phenotypes at 25C Paper_evidence: WBPaper00026928
            Curator_confirmed: WBPerson2021
        Variation_effect: Null: Paper_evidence: WBPaper00026928
            Curator_confirmed: WBPerson2021
        EQ_annotations: Life_stage: WBls:0000003 PATO:0000460 Paper_evidence: WBPaper00026928
                Curator_confirmed: WBPerson2021
        Temperature_sensitive: Heat_sensitive: 25C Paper_evidence: WBPaper00026928
              Curator_confirmed: WBPerson2021
        Phenotype_assay: Temperature: 25C Paper_evidence: WBPaper00026928
              Curator_confirmed: WBPerson2021
      WBPhenotype:0001355 Paper_evidence: WBPaper00026928
        Curator_confirmed: WBPerson2021
        Remark: cnx-1(nr2009) mutant gonad showed much reduced yolk granules compared to wild type Paper_evidence: WBPaper00026928
            Curator_confirmed: WBPerson2021
        Variation_effect: Null: Paper_evidence: WBPaper00026928
            Curator_confirmed: WBPerson2021
        Phenotype_assay: Treatment: Immunogold electron micrographs (CNX-1 antibody) Paper_evidence: WBPaper00026928
              Curator_confirmed: WBPerson2021
      WBPhenotype:0001428 Paper_evidence: WBPaper00026928
        Curator_confirmed: WBPerson2021
        Remark: Severe morphological defects were observed in the intestine of mutant embryos (vacuolated intestine) at 25C Paper_evidence: WBPaper00026928
            Curator_confirmed: WBPerson2021
        Variation_effect: Null: Paper_evidence: WBPaper00026928
            Curator_confirmed: WBPerson2021
        EQ_annotations: Life_stage: WBls:0000003 PATO:0000460 Paper_evidence: WBPaper00026928
                Curator_confirmed: WBPerson2021
        Temperature_sensitive: Heat_sensitive: 25C Paper_evidence: WBPaper00026928
              Curator_confirmed: WBPerson2021
        Phenotype_assay: Temperature: 25C Paper_evidence: WBPaper00026928
              Curator_confirmed: WBPerson2021
      WBPhenotype:0001485 Paper_evidence: WBPaper00026928
        Curator_confirmed: WBPerson2021
        Remark: Mutant animals are hypersensitive to EGTA and DTT (inducers of ER stress) Paper_evidence: WBPaper00026928
            Curator_confirmed: WBPerson2021
        Variation_effect: Null: Paper_evidence: WBPaper00026928
            Curator_confirmed: WBPerson2021
        Phenotype_assay: Treatment: 0-10mM of EGTA or DTT Paper_evidence: WBPaper00026928
              Curator_confirmed: WBPerson2021
      WBPhenotype:0001724 Paper_evidence: WBPaper00026928
        Curator_confirmed: WBPerson2021
        Remark: Mutants were severely affected by tunicamycin Paper_evidence: WBPaper00026928
            Curator_confirmed: WBPerson2021
        Variation_effect: Null: Paper_evidence: WBPaper00026928
            Curator_confirmed: WBPerson2021
        Phenotype_assay: Treatment: 0-7.5 ug/ml of tunicamycin Paper_evidence: WBPaper00026928
              Curator_confirmed: WBPerson2021
      WBPhenotype:0002606 Paper_evidence: WBPaper00041673
        Curator_confirmed: WBPerson2987
        Remark: "Likewise, loss of crt-1, cnx-1, itr-1, clp-1, tra-3, asp-3, or asp-4 partially suppressed HBx-induced cell killing (from 50% to 12-32% PLM death; Fig. 2A), indicating that HBx induces cell death in part through the necrotic pathway." Paper_evidence: WBPaper00041673
            Curator_confirmed: WBPerson2987
        Variation_effect: Hypomorph_reduction_of_function: Paper_evidence: WBPaper00041673
            Curator_confirmed: WBPerson2987
        Phenotype_assay: Genotype: smIs98 [Pmec-3::GFP; Pmec-7::HBx] Paper_evidence: WBPaper00041673
              Curator_confirmed: WBPerson2987
    Phenotype_not_observed: WBPhenotype:0000039 Paper_evidence: WBPaper00026928
        Curator_confirmed: WBPerson2021
        Remark: Movement and life span appeared comparable to wild-type animals Paper_evidence: WBPaper00026928
            Curator_confirmed: WBPerson2021
        Variation_effect: Null: Paper_evidence: WBPaper00026928
            Curator_confirmed: WBPerson2021
      WBPhenotype:0000071 Paper_evidence: WBPaper00026928
        Curator_confirmed: WBPerson2021
        Remark: No obvious defect in head morphology Paper_evidence: WBPaper00026928
            Curator_confirmed: WBPerson2021
        Variation_effect: Null: Paper_evidence: WBPaper00026928
            Curator_confirmed: WBPerson2021
      WBPhenotype:0000073 Paper_evidence: WBPaper00026928
        Curator_confirmed: WBPerson2021
        Remark: No obvious defect in tail morphology Paper_evidence: WBPaper00026928
            Curator_confirmed: WBPerson2021
        Variation_effect: Null: Paper_evidence: WBPaper00026928
            Curator_confirmed: WBPerson2021
      WBPhenotype:0000643 Paper_evidence: WBPaper00026928
        Curator_confirmed: WBPerson2021
        Remark: Movement and life span appeared comparable to wild-type animals Paper_evidence: WBPaper00026928
            Curator_confirmed: WBPerson2021
        Variation_effect: Null: Paper_evidence: WBPaper00026928
            Curator_confirmed: WBPerson2021
      WBPhenotype:0000704 Paper_evidence: WBPaper00026928
        Curator_confirmed: WBPerson2021
        Remark: No obvious defect in excretory canal structure Paper_evidence: WBPaper00026928
            Curator_confirmed: WBPerson2021
        Variation_effect: Null: Paper_evidence: WBPaper00026928
            Curator_confirmed: WBPerson2021
  Reference: WBPaper00026928
    WBPaper00010060
    WBPaper00041673
  Remark: nr2009 deletes 442bp from the 3' of exon 1 to the 5' of exon 3. Specific coordinates are not given in the paper. Paper_evidence: WBPaper00026928
    Manually curated Gene associations preserved as a text remark so that VEP is the canonical predictor of consequence: WBGene00000567 Intron Paper_evidence: WBPaper00026928
    Manually curated Gene associations preserved as a text remark so that VEP is the canonical predictor of consequence: WBGene00000567 Coding_exon Paper_evidence: WBPaper00026928
  Method: Deletion_allele