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WormBase Tree Display for Variation: WBVar00090602

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Name Class

WBVar00090602EvidencePaper_evidenceWBPaper00003929
NamePublic_namen2853
Sequence_detailsSMapS_parentSequenceC05G5
Flanking_sequencesgtggacggtctacactgtggatccggtgagtagtaggttgtatagtttggaatattacca
Mapping_targetC05G5
Type_of_mutationSubstitutiongaPaper_evidenceWBPaper00003929
SeqStatusSequenced
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00027302
WBStrain00029077
LaboratoryMT
StatusLive
AffectsGeneWBGene00002285
TranscriptC05G5.6
Interactor (53)
GeneticsInterpolated_map_positionX21.2123
DescriptionPhenotypeWBPhenotype:0000033Paper_evidenceWBPaper00031335
WBPaper00003929
WBPaper00036739
Curator_confirmedWBPerson2021
WBPerson712
WBPerson3490
RemarkHeterochronic defectPaper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
n2853 caused the strongest retarded heterochronic defects in a lin14(+) background among 36 identified suppressors of the lin-14 and egl-35 synthetic sterile phenotype. In let-7(n2853) animals, the blast cell lineages were normal through the L3-to-L4 moult, but at the L4-to-adult moult, they reiterated larval patterns of cell division. LIN-29 expression in the hypodermis of L4 stage let-7 animals was reduced relative to wild type, but expressed at normal levels at the L5 stage.Paper_evidenceWBPaper00003929
Curator_confirmedWBPerson712
let-7(n2853) mutant animals reared at the permissive temperature underwent a supernumerary moult to a 5th larval stage, L5 (56%, n= 26). At the L5-to-adult moult, seam cells exited the cell cycle, fused with neighbouring seam cells, and produced alae (100%, n = 10 animals).Paper_evidenceWBPaper00003929
Curator_confirmedWBPerson712
Variation_effectProbable_null_via_phenotypePaper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
Temperature_sensitiveHeat_sensitivePaper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
WBPhenotype:0000038Paper_evidenceWBPaper00003929
WBPaper00029255
WBPaper00034755
Curator_confirmedWBPerson712
RemarkThe n2853 ts lethal phenotype is associated with vulval bursting.Paper_evidenceWBPaper00003929
Curator_confirmedWBPerson712
let-7(n2853) temperature sensitive mutant animals, in which the mutated let-7 miRNA contains a G to A base substitution at the fifth nucleotide, die at the young adult stage by bursting through the vulva at the non-permissive temperature (20 C). The phenotype is less penetrant at a lower temperature (15 C).Paper_evidenceWBPaper00034755
Curator_confirmedWBPerson712
Temperature_sensitiveHeat_sensitive20Paper_evidenceWBPaper00034755
Curator_confirmedWBPerson712
WBPhenotype:0000054Paper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
RemarkAnimals die as larvaePaper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
Variation_effectProbable_null_via_phenotypePaper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
Temperature_sensitiveHeat_sensitivePaper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
WBPhenotype:0000060Paper_evidenceWBPaper00003929
Curator_confirmedWBPerson712
Remarkn2853 caused a temperature-sensitive adult lethal phenotype.Paper_evidenceWBPaper00003929
Curator_confirmedWBPerson712
EQ_annotationsAnatomy_termWBbt:0007833PATO:0001422Paper_evidenceWBPaper00003929
Curator_confirmedWBPerson712
Life_stageWBls:0000041PATO:0000460Paper_evidenceWBPaper00003929
Curator_confirmedWBPerson712
Temperature_sensitiveHeat_sensitivePaper_evidenceWBPaper00003929
Curator_confirmedWBPerson712
WBPhenotype:0000062Paper_evidenceWBPaper00031590
WBPaper00029255
Curator_confirmedWBPerson712
RemarkAnimals produced no live progeny from 10 adult worms.Paper_evidenceWBPaper00031590
Curator_confirmedWBPerson712
At the permissive temperature (15 deg C), let-7(n2853) animals, on mock RNAi, exhibit 87% survival.Paper_evidenceWBPaper00029255
Curator_confirmedWBPerson712
Variation_effectProbable_null_via_phenotypePaper_evidenceWBPaper00031590
Curator_confirmedWBPerson712
Phenotype_assayTemperature25CPaper_evidenceWBPaper00031590
Curator_confirmedWBPerson712
WBPhenotype:0000114Paper_evidenceWBPaper00032489
Curator_confirmedWBPerson712
Remarkugt-63 and vit-1 are differentially expressed in synchronized late L4 wild-type and let-7(n2853) animals but are not direct targets of let-7 (B Hurschler and HG, unpublished data); vit-1 was four-fold less abundant in let-7(n2853) than in wild-type animals, and ugt-63 was two-fold more abundant however, the translational profiles of both genes were similar in wild-type and let-7(n2853).Paper_evidenceWBPaper00032489
Curator_confirmedWBPerson712
EQ_annotationsLife_stageWBls:0000038PATO:0000460Paper_evidenceWBPaper00032489
Curator_confirmedWBPerson712
WBPhenotype:0000134Paper_evidenceWBPaper00036739
Curator_confirmedWBPerson3490
WBPhenotype:0000135Paper_evidenceWBPaper00003929
WBPaper00036739
Curator_confirmedWBPerson712
WBPerson3490
RemarkThe lacZ/lin-41 3' UTR fusion gene was expressed in 79% (n = 14) of let-7 (n2853) adult animals but only 19% (n = 21) of wild-type adults. While no major alterations in the timing or levels of LIN-14 (data not shown) or lin-28::GFP expression (V. Ambros, personal communication) were observed in let-7 mutant animals.Paper_evidenceWBPaper00003929
Curator_confirmedWBPerson712
WBPhenotype:0000136Paper_evidenceWBPaper00026761
Curator_confirmedWBPerson712
RemarkA greater than 3-fold higher level of lin-41 in RNA samples were collected from let-7(n2853) compared to wild-type worms at the L4 stage. Relative lin-41 mRNA levels were indistinguishable between wild-type and let-7(n2853) worms at the L2 stage, which is prior to the onset of let-7 expression. The expression levels of con- trol mRNAs not predicted to be regulated by let-7, including eft-2, unc-54 and col-10, were indistinguishable in wild-type versus let-7(n2853) worms during larval development.Paper_evidenceWBPaper00026761
Curator_confirmedWBPerson712
WBPhenotype:0000167Paper_evidenceWBPaper00036739
Curator_confirmedWBPerson3490
WBPhenotype:0000170Paper_evidenceWBPaper00036739
Curator_confirmedWBPerson3490
WBPhenotype:0000438Paper_evidenceWBPaper00031590
Curator_confirmedWBPerson712
RemarkNo adult lateral alae were present in L4 (n=20) or 0% adults (n=20).Paper_evidenceWBPaper00031590
Curator_confirmedWBPerson712
Variation_effectProbable_null_via_phenotypePaper_evidenceWBPaper00031590
Curator_confirmedWBPerson712
EQ_annotationsAnatomy_termWBbt:0007832PATO:0000460Paper_evidenceWBPaper00031590
Curator_confirmedWBPerson712
Phenotype_assayTemperature15CPaper_evidenceWBPaper00031590
Curator_confirmedWBPerson712
WBPhenotype:0000638Paper_evidenceWBPaper00031590
Curator_confirmedWBPerson712
Variation_effectProbable_null_via_phenotypePaper_evidenceWBPaper00031590
Curator_confirmedWBPerson712
Phenotype_assayTemperature15CPaper_evidenceWBPaper00031590
Curator_confirmedWBPerson712
WBPhenotype:0000640Paper_evidenceWBPaper00031590
Curator_confirmedWBPerson712
Variation_effectProbable_null_via_phenotypePaper_evidenceWBPaper00031590
Curator_confirmedWBPerson712
Phenotype_assayTemperature15CPaper_evidenceWBPaper00031590
Curator_confirmedWBPerson712
WBPhenotype:0000688Paper_evidenceWBPaper00031590
Curator_confirmedWBPerson712
RemarkAnimals produced 2-5% fertile eggs (n>100).Paper_evidenceWBPaper00031590
Curator_confirmedWBPerson712
Variation_effectProbable_null_via_phenotypePaper_evidenceWBPaper00031590
Curator_confirmedWBPerson712
Phenotype_assayTemperature25CPaper_evidenceWBPaper00031590
Curator_confirmedWBPerson712
WBPhenotype:0000962Paper_evidenceWBPaper00034755
Curator_confirmedWBPerson712
RemarkX-Gal staining of let-7(n2853) adult animals was unchanged from larval levels (98%, n = 359, normalized to stained larvae), unlike controls.Paper_evidenceWBPaper00034755
Curator_confirmedWBPerson712
Haplo_insufficientPaper_evidenceWBPaper00034755
Curator_confirmedWBPerson712
Phenotype_assayGenotypemgEx540[Pcol-10::Lac-Z::lin-41-3'UTR]Paper_evidenceWBPaper00034755
Curator_confirmedWBPerson712
WBPhenotype:0001228Paper_evidenceWBPaper00003929
Curator_confirmedWBPerson712
Remarkn2853 mutants failed to generate alae.Paper_evidenceWBPaper00003929
Curator_confirmedWBPerson712
WBPhenotype:0001236Paper_evidenceWBPaper00042320
Curator_confirmedWBPerson712
Remarkhbl-1::gfp::hbl-1 in VNC and col-10::gfp::lin-41 (3'UTR) in hypodermal cells had elevated expression. This elevated expression was not affected by loss of autophagy activity.Paper_evidenceWBPaper00042320
Curator_confirmedWBPerson712
WBPhenotype:0001431Paper_evidenceWBPaper00027716
Curator_confirmedWBPerson48
PenetranceRange5959Paper_evidenceWBPaper00027716
Curator_confirmedWBPerson48
Phenotype_assayTemperature15Paper_evidenceWBPaper00027716
Curator_confirmedWBPerson48
Genotypen2853; him-5(e1490)Paper_evidenceWBPaper00027716
Curator_confirmedWBPerson48
WBPhenotype:0001779Paper_evidenceWBPaper00026761
Curator_confirmedWBPerson712
RemarkThe n2853 mutation results in significantly lower levels of the mature let-7 miRNA; Northern analyses showed that let-7 levels at the L4 stage in the mutant were lower by over 3-fold at 15C and over 10-fold at 25C compared to wild-type.Paper_evidenceWBPaper00026761
Curator_confirmedWBPerson712
WBPhenotype:0001801Paper_evidenceWBPaper00056872
Curator_confirmedWBPerson26767
Remarkaxodendritic polarity of DA9 neuron variantPaper_evidenceWBPaper00056872
Curator_confirmedWBPerson26767
EQ_annotationsAnatomy_termWBbt:0004857PATO:0000460Paper_evidenceWBPaper00056872
Curator_confirmedWBPerson26767
Temperature_sensitiveHeat_sensitivePaper_evidenceWBPaper00056872
Curator_confirmedWBPerson26767
Phenotype_assayGenotypewyIs85 [Pitr-1-pB::GFP::RAB-3]Paper_evidenceWBPaper00056872
Curator_confirmedWBPerson26767
WBPhenotype:0001887Paper_evidenceWBPaper00034755
Curator_confirmedWBPerson712
RemarkIn let-7(n2853) animals, seam cells reiterate the late larval fate of cell division at the L4-to-adult molt, which leads to an increased number of seam cells. let- 7(n2853) animals after the L4-to-adult molt displayed ~22.3 seam cell nuclei (n = 110) on average.Paper_evidenceWBPaper00034755
Curator_confirmedWBPerson712
Haplo_insufficientPaper_evidenceWBPaper00034755
Curator_confirmedWBPerson712
WBPhenotype:0002154Paper_evidenceWBPaper00032489
Curator_confirmedWBPerson712
RemarkBoth lin-41 and daf-12 mRNAs were moderately, but consistently depleted from the highly translated polysomal fractions in wild-type relative to let-7 mutant animals at the late L4 stage. By contrast, ama-1 and act-1 mRNAs, which are not targeted by let-7, displayed similar translational profiles in both strains. 40% of reporter mRNA was associated with polysomes in wild-type animals, whereas this level reached almost 70% in let-7 mutant animals. Consistent with inhibition of translation initiation, we observed that only 40% of the reporter mRNA was associated with polysomes in wild-type animals, whereas this level reached almost 70% in let-7 mutant animals. The let-7 mutation increased the average number of ribosomes per lacZ mRNA by more than two-fold relative to the wild-type situation.Paper_evidenceWBPaper00032489
Curator_confirmedWBPerson712
Rescued_by_transgeneWBTransgene00005568
WBPhenotype:0002157Paper_evidenceWBPaper00032489
Curator_confirmedWBPerson712
RemarkAt the late L4 stage, lin-41 mRNA is six-fold and daf-12 two-fold more abundant in let-7(n2853) relative to wild-type animals, but not at the L3 stage.Paper_evidenceWBPaper00032489
Curator_confirmedWBPerson712
EQ_annotationsLife_stageWBls:0000038PATO:0000460Paper_evidenceWBPaper00032489
Curator_confirmedWBPerson712
WBPhenotype:0002479Paper_evidenceWBPaper00042278
Curator_confirmedWBPerson17616
Phenotype_not_observedWBPhenotype:0000207Paper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
RemarkDefecation cycle is normalPaper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
Variation_effectProbable_null_via_phenotypePaper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
WBPhenotype:0000634Paper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
RemarkPumping is normalPaper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
Variation_effectProbable_null_via_phenotypePaper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
WBPhenotype:0000637Paper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
RemarkNo defects in dauer formationPaper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
Variation_effectProbable_null_via_phenotypePaper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
WBPhenotype:0000643Paper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
Remarknon-UncPaper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
Variation_effectProbable_null_via_phenotypePaper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
WBPhenotype:0001233Paper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
RemarkCell number and nuclear staining is normalPaper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
Variation_effectProbable_null_via_phenotypePaper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
Phenotype_assayTreatmentDAPI stainingPaper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
WBPhenotype:0002535Paper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
Remarknon-DyfPaper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
Variation_effectProbable_null_via_phenotypePaper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
EQ_annotationsAnatomy_termWBbt:0005666PATO:0000460Paper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
WBbt:0005667PATO:0000460Paper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
WBbt:0005668PATO:0000460Paper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
WBbt:0005665PATO:0000460Paper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
WBbt:0005661PATO:0000460Paper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
WBbt:0007807PATO:0000460Paper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
WBbt:0007808PATO:0000460Paper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
Phenotype_assayTreatmentDiO stainingPaper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
Reference (18)
Remark
MethodSubstitution_allele