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WormBase Tree Display for Variation: WBVar00088385

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Name Class

WBVar00088385EvidencePerson_evidenceWBPerson9765
NamePublic_nameky5
Other_nameZK512.6a.1:c.15_326del
CE01109:p.Glu6_Ter109delextTer?
HGVSgCHROMOSOME_III:g.9136930_9137543del
Sequence_detailsSMapS_parentSequenceZK512
Flanking_sequencesTCATTTTCAGAAACCATGTCGTCATGGAATGTAAGACACGATAAAAAAATTATGCAAAA
Mapping_targetZK512
Type_of_mutationDeletion
SeqStatusSequenced
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00005282
WBStrain00005551
WBStrain00005552
WBStrain00005553
WBStrain00027259
LaboratoryCX
StatusLive
AffectsGeneWBGene00001135
TranscriptZK512.6b.1VEP_consequencesplice_acceptor_variant,splice_donor_variant,coding_sequence_variant,5_prime_UTR_variant,intron_variant
VEP_impactHIGH
cDNA_position?-263
CDS_position?-263
Protein_position?-88
Intron_number1/10
Exon_number1-2/11
ZK512.6a.1 (11)
InteractorWBInteraction000578897
WBInteraction000578898
WBInteraction000578899
GeneticsInterpolated_map_positionIII0.17242
DescriptionPhenotypeWBPhenotype:0000019Paper_evidenceWBPaper00032082
Curator_confirmedWBPerson712
RemarkAnimals exhibited reduced pumping rates similar to daf-7(e1372).Paper_evidenceWBPaper00032082
Curator_confirmedWBPerson712
Phenotype_assayTreatmentPumping rates were assayed for well-fed , pheromone-untreated; food-deprived, pheromone-untreated; and well-fed, pheromone-treated animals. Pheromone treatment consisted of transfer of animals grown under well-fed conditions to plates containing 30 ul/ml of a dauer-pheromone prep (Golden and Riddle, 1982) and 30 ml of OP50 E. coli food.Paper_evidenceWBPaper00032082
Curator_confirmedWBPerson712
Temperature20Paper_evidenceWBPaper00032082
Curator_confirmedWBPerson712
WBPhenotype:0000412Paper_evidenceWBPaper00003408
Curator_confirmedWBPerson557
WBPhenotype:0000478Paper_evidenceWBPaper00038117
Curator_confirmedWBPerson712
RemarkNo mutants exhibited isothermal tracking behaviour.Paper_evidenceWBPaper00038117
Curator_confirmedWBPerson712
WBPhenotype:0000556Paper_evidenceWBPaper00006375
Curator_confirmedWBPerson712
RemarkAnimals did not modulate their ARS frequency of high-angled turns in the presence of dopamine unlike wild-type worms.Paper_evidenceWBPaper00006375
Curator_confirmedWBPerson712
WBPhenotype:0000662Paper_evidenceWBPaper00006375
Curator_confirmedWBPerson712
RemarkARS defective. Animals do not exhibit a decrease in the frequency of high-angled turns in response to food deprivation.Paper_evidenceWBPaper00006375
Curator_confirmedWBPerson712
WBPhenotype:0000663Paper_evidenceWBPaper00003408
Curator_confirmedWBPerson557
WBPhenotype:0000999Paper_evidenceWBPaper00038117
Curator_confirmedWBPerson712
RemarkAnimals exhibited little tendency to migrate towards cultivation temperature and dispersed more broadly from cultivation temperature than wild-type animals on a thermal gradient.Paper_evidenceWBPaper00038117
Curator_confirmedWBPerson712
WBPhenotype:0001049Paper_evidenceWBPaper00048845
Curator_confirmedWBPerson3990
Remarkreduced sensitivity in acidic pH avoidancePaper_evidenceWBPaper00048845
Curator_confirmedWBPerson3990
WBPhenotype:0001221Paper_evidenceWBPaper00003408
Curator_confirmedWBPerson557
WBPhenotype:0001444Paper_evidenceWBPaper00032335
Curator_confirmedWBPerson712
RemarkAnimals did not show avoidance to NaCl after pre-exposure.Paper_evidenceWBPaper00032335
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00003571Paper_evidenceWBPaper00032335
Curator_confirmedWBPerson712
Phenotype_assayTreatmentAnimals were tested for response to 25 mM NaCl after various pre-treatments: a 15-min wash in CTX buffer with or without 100 mM NaCl (liquid), or 30 min on a CTX plate with or without 100 mM NaCl, and in the presence or absence of bacteria, 500 mM glycerol, or 3 uL of benzaldehyde.Paper_evidenceWBPaper00032335
Curator_confirmedWBPerson712
WBPhenotype:0001462Paper_evidenceWBPaper00032335
Curator_confirmedWBPerson712
RemarkAnimals showed strong chemotaxis defects compared to wild type.Paper_evidenceWBPaper00032335
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00003571Paper_evidenceWBPaper00032335
Curator_confirmedWBPerson712
Phenotype_assayTreatmentAnimals were tested for response to 25 mM NaCl.Paper_evidenceWBPaper00032335
Curator_confirmedWBPerson712
WBPhenotype:0001983Paper_evidenceWBPaper00060628
Curator_confirmedWBPerson712
Remarkeat-4 mutants were significantly enhanced food leavers (eat-4(ky5) (Fig. 1B, See time course over 60 minutes, Lee et al., 1999). eat-4(ky5) mutants show a significant increase in the number of worms that were not on the food at time points across 15, 30, 45 and 60 minutes (Fig. 1B).Paper_evidenceWBPaper00060628
Curator_confirmedWBPerson712
WBPhenotype:0002545Paper_evidenceWBPaper00060628
Curator_confirmedWBPerson712
Remarkeat-4 mutants again did show an obvious food leaving phenotype when compared to wild type animals on Comamonas sp food lawns (eat-4(ky5) tested), Fig. 1D). We examined strains that selectively rescue eat-4 in specific sets of glutamatergic neurons. eat-4 is expressed in over 30 neurons in the hermaphrodite, including, sensory and interneurons (Serrano-Saiz et al., 2013). We examined eat-4 mutants that were rescued using the odr-3 promoter that expresses strongly in the AWC sensory neurons, weakly in the AWB sensory neurons and faintly in the AWA, ASH and ADF sensory neurons (Royaie et al., 1998, Chalasani et al., 2007, Calhoun et al., 2015, Fig. 1F). Interestingly, eat-4 rescue using an odr-3 promoter rescued eat-4(ky5) mutants for staying on a food patch (Fig. 1F).Paper_evidenceWBPaper00060628
Curator_confirmedWBPerson712
Rescued_by_transgeneWBTransgene00016436
WBPhenotype:0002568Paper_evidenceWBPaper00005404
Curator_confirmedWBPerson10038
Remarkquoted from paper: "When the mean reversal magnitudes to the 10 test single-taps were compared between the trained group and their single-tap controls (t(42)=0.38, P=0.71; Fig. 5), the eat-4 worms showed no evidence of long-term memory for the habituation to tap training."Paper_evidenceWBPaper00005404
Curator_confirmedWBPerson10038
Rescued_by_transgeneWBTransgene00016287
Phenotype_assayControl_strainWBStrain00000001Paper_evidenceWBPaper00005404
Curator_confirmedWBPerson10038
Phenotype_not_observedWBPhenotype:0000006Paper_evidenceWBPaper00032082
Curator_confirmedWBPerson712
RemarkAll these animals retained a similar number of eggs when compared to wild-type animals (~10 eggs).Paper_evidenceWBPaper00032082
Curator_confirmedWBPerson712
Phenotype_assayTreatmentYoung well-fed animals were scoredPaper_evidenceWBPaper00032082
Curator_confirmedWBPerson712
Temperature25Paper_evidenceWBPaper00032082
Curator_confirmedWBPerson712
WBPhenotype:0000631Paper_evidenceWBPaper00035198
Curator_confirmedWBPerson712
RemarkAnimals do not exhibit anterior convulsions when treated with pentylenetetrazole(PTZ).Paper_evidenceWBPaper00035198
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00004251Paper_evidenceWBPaper00035198
Curator_confirmedWBPerson712
WBPhenotype:0000643Paper_evidenceWBPaper00038117
Curator_confirmedWBPerson712
Remarkeat-4(ky5) mutants did not show locomotion defects.Paper_evidenceWBPaper00038117
Curator_confirmedWBPerson712
WBPhenotype:0001182Paper_evidenceWBPaper00032082
Curator_confirmedWBPerson712
RemarkFat levels were similar to that seen in wild-type but are reduced when compared to daf-7(e1375) as determined by Sudan Black assay (described in Kimura et al., 1997).Paper_evidenceWBPaper00032082
Curator_confirmedWBPerson712
Phenotype_assayTreatmentTo minimize staining variability, which allowed for quantitative comparisons between various genotypes: animals from one genotype were labeled with fluorescein isothiocyanate (FITC) and then fixed and stained in the same tube as unlabeled animals from another genotype.Paper_evidenceWBPaper00032082
Curator_confirmedWBPerson712
Temperature22Paper_evidenceWBPaper00032082
Curator_confirmedWBPerson712
WBPhenotype:0001291Paper_evidenceWBPaper00038117
Curator_confirmedWBPerson712
RemarkAnimals dispersed less than wild-type animals in the absence of a temperature gradient.Paper_evidenceWBPaper00038117
Curator_confirmedWBPerson712
WBPhenotype:0001765Paper_evidenceWBPaper00031936
Curator_confirmedWBPerson2021
RemarkMutants defective in the synthesis and reception of nonessential excitatory neurotransmitters respond normally to CO2Paper_evidenceWBPaper00031936
Curator_confirmedWBPerson2021
EQ_annotationsLife_stageWBls:0000057PATO:0000460Paper_evidenceWBPaper00031936
Curator_confirmedWBPerson2021
Phenotype_assayTreatment10% CO2Paper_evidenceWBPaper00031936
Curator_confirmedWBPerson2021
WBPhenotype:0001780Paper_evidenceWBPaper00029060
Curator_confirmedWBPerson2021
RemarkMutants show normal butanone enhancementPaper_evidenceWBPaper00029060
Curator_confirmedWBPerson2021
EQ_annotationsLife_stageWBls:0000057PATO:0000460Paper_evidenceWBPaper00029060
Curator_confirmedWBPerson2021
Phenotype_assayTreatmentPreexposure to 1:10 dilution of butanone and food. Animals were then subjected to odorant chemotaxis assays and/or selection assaysPaper_evidenceWBPaper00029060
Curator_confirmedWBPerson2021
WBPhenotype:0002469Paper_evidenceWBPaper00005404
Curator_confirmedWBPerson10038
Remarkquoted from paper: "Figure 4A shows the average response magnitudes for reversals in the three blocks of training on day 1 and the test block on day 2. When stimulated with trains of taps, the eat-4 worms showed normal short term habituation over each block of stimuli."Paper_evidenceWBPaper00005404
Curator_confirmedWBPerson10038
Phenotype_assayControl_strainWBStrain00000001Paper_evidenceWBPaper00005404
Curator_confirmedWBPerson10038
WBPhenotype:0002568Paper_evidenceWBPaper00005404
Curator_confirmedWBPerson10038
Remarkquoted from paper: "Using the new group-training procedure with the stronger train of taps resulted in significant long-term memory for habituation training in the group of eat-4 mutants that received distributed training (t(34)=2.54, P<0.05) when compared with the single train control group (Fig. 5)."Paper_evidenceWBPaper00005404
Curator_confirmedWBPerson10038
Phenotype_assayControl_strainWBStrain00000001Paper_evidenceWBPaper00005404
Curator_confirmedWBPerson10038
Reference (19)
MethodDeletion_allele