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WormBase Tree Display for Variation: WBVar00000105

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Name Class

WBVar00000105EvidencePaper_evidenceWBPaper00005370
NamePublic_nameag3
Other_nameCE44901:p.Gln1003Ter
F59A6.1a.1:c.3007C>T
F59A6.1b.1:c.2956C>T
CE50862:p.Gln986Ter
HGVSgCHROMOSOME_II:g.5027769C>T
Sequence_detailsSMapS_parentSequenceF33G12
Flanking_sequencescttactgctggattctctcatgttcacagtaaactgtatcgaatgcattgtcaactgcac
Mapping_targetF33G12
Type_of_mutationSubstitutionctPaper_evidenceWBPaper00005370
Person_evidenceWBPerson2292
SeqStatusSequenced
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00000259
WBStrain00055931
LaboratoryAU
JN
StatusLive
AffectsGeneWBGene00003822
TranscriptF59A6.1a.1VEP_consequencestop_gained
VEP_impactHIGH
HGVScF59A6.1a.1:c.3007C>T
HGVSpCE44901:p.Gln1003Ter
cDNA_position3164
CDS_position3007
Protein_position1003
Exon_number9/12
Codon_changeCaa/Taa
Amino_acid_changeQ/*
F59A6.1b.1VEP_consequencestop_gained
VEP_impactHIGH
HGVScF59A6.1b.1:c.2956C>T
HGVSpCE50862:p.Gln986Ter
cDNA_position2956
CDS_position2956
Protein_position986
Exon_number8/11
Codon_changeCaa/Taa
Amino_acid_changeQ/*
GeneticsInterpolated_map_positionII-2.59195
DescriptionPhenotypeWBPhenotype:0000006Paper_evidenceWBPaper00005370
Curator_confirmedWBPerson712
WBPhenotype:0000386Paper_evidenceWBPaper00033433
WBPaper00035318
Curator_confirmedWBPerson2987
RemarkCopper-induced germ cell apoptosis was abolished in nsy-1(ag3) mutants (Figure 5A).Paper_evidenceWBPaper00033433
Curator_confirmedWBPerson2987
Cobalt did not induce increased germ cell apoptosis in nsy-1(ag3) mutants, as opposed to wild type animals (Table 2)Paper_evidenceWBPaper00035318
Curator_confirmedWBPerson2987
Affected_byMoleculeWBMol:00002862Paper_evidenceWBPaper00033433
Curator_confirmedWBPerson2987
WBMol:00003058Paper_evidenceWBPaper00035318
Curator_confirmedWBPerson2987
Phenotype_assayTreatmentWorms were exposed to 10 micromolar of copper for 12 hoursPaper_evidenceWBPaper00033433
Curator_confirmedWBPerson2987
Synchronized young adult hermaphrodites were treated in K-medium with 0.01 millimolar Cobalt chloride for 12 hours, and apoptotic cells were scored after AO staining.Paper_evidenceWBPaper00035318
Curator_confirmedWBPerson2987
WBPhenotype:0001012Paper_evidenceWBPaper00055320
Curator_confirmedWBPerson557
RemarkThis mutant showed no protection from enterotoxigenic Escherichia coli (ETEC) infection when pretreated with Lactobacillus zeae LB1. Authors show that wild type worms had "enhancement in the resistance" to infection by ETEC when worms were first grown on Lactobacillus zeae LB1. This "enhancement in resistance" is not observed in this mutant.Paper_evidenceWBPaper00055320
Curator_confirmedWBPerson557
Phenotype_assayTreatmentAnimals were grown on plates containing K88plus enterotoxigenic Escherichia coli(ETEC) strain JG280 after pretreatment with Lactobacillus zeae LB1.Paper_evidenceWBPaper00055320
Curator_confirmedWBPerson557
WBPhenotype:0001013Paper_evidenceWBPaper00005370
WBPaper00055320
WBPaper00061439
Curator_confirmedWBPerson712
WBPerson557
WBPerson49407
RemarkWhen exposed to Pseudomonas aeruginosa strain, PA14, under standard assay conditions, 80% esp-8 animals were killed by 31 hours, compared with 0% wild-type animals. Accumulation of GFP-labeled PA14 was detected as early as 6 hours after exposure. Animals were also hypersusceptible to Enterococcus faecalis.Paper_evidenceWBPaper00005370
Curator_confirmedWBPerson712
This mutant is more susceptible to enterotoxigenic Escherichia coli infection compared with N2 wild-type animals.Paper_evidenceWBPaper00055320
Curator_confirmedWBPerson557
Phenotype_assayTreatmentApproximately 25 L4-stage worms were placed on each of three agar plates with PA14 lawns under standard slow killing (SK) assay conditions. Survival was assayed after 30 hours.Paper_evidenceWBPaper00005370
Curator_confirmedWBPerson712
Animals grown on plates containing K88plus enterotoxigenic Escherichia coli (ETEC) strain JG280.Paper_evidenceWBPaper00055320
Curator_confirmedWBPerson557
WBPhenotype:0001351Paper_evidenceWBPaper00005370
Curator_confirmedWBPerson712
RemarkAnimals had diminished levels of p38 MAP kinase activation compared to wild-type N2 worms, when in the presence of PA14, as determined by immunoblot analysis of lysates from mutants probed with anti-phospho-p38 and anti-PMK-1 with anti-beta-tubulin as a loading control.Paper_evidenceWBPaper00005370
Curator_confirmedWBPerson712
Phenotype_assayTreatmentSynchronized L4 populations of N2 wild type, esp-2/sek-1(ag1), and esp-8/nsy-1(ag3) worms were transferred to PA14 plates in parallel, and lysates were prepared for immunoblotting after 6 hours when the worms were predominantly young adults.Paper_evidenceWBPaper00005370
Curator_confirmedWBPerson712
WBPhenotype:0001381Paper_evidenceWBPaper00037959
Curator_confirmedWBPerson712
RemarkMutant animals showed higher survival rates under anoxic conditions than the wild-type allele.Paper_evidenceWBPaper00037959
Curator_confirmedWBPerson712
WBPhenotype:0001687Paper_evidenceWBPaper00056945
Curator_confirmedWBPerson712
Remarknsy-1(ag3) animals showed staining of L2-L4 stages. mAb M37 stains only the L1 stage in wild type C. elegans under normal growth conditions.Paper_evidenceWBPaper00056945
Curator_confirmedWBPerson712
ImageWBPicture0000014905Paper_evidenceWBPaper00056945
Curator_confirmedWBPerson712
WBPhenotype:0001857Paper_evidenceWBPaper00035315
Curator_confirmedWBPerson2021
RemarkNo induction of Ptph-1::GFP expression by PA14 in the nsy-1 mutant backgroundPaper_evidenceWBPaper00035315
Curator_confirmedWBPerson2021
Phenotype_not_observedWBPhenotype:0000039Paper_evidenceWBPaper00005370
Curator_confirmedWBPerson712
RemarkLongevity of animals was equivalent to wild-type worms.Paper_evidenceWBPaper00005370
Curator_confirmedWBPerson712
Phenotype_assayTreatment25 L4 animals were placed on each of three plates that contained 5-fluoro- 2-deoxyuridine and had been seeded with E. coli OP50.Paper_evidenceWBPaper00005370
Curator_confirmedWBPerson712
WBPhenotype:0000650Paper_evidenceWBPaper00005370
Curator_confirmedWBPerson712
WBPhenotype:0000659Paper_evidenceWBPaper00005370
Curator_confirmedWBPerson712
WBPhenotype:0000676Paper_evidenceWBPaper00005370
Curator_confirmedWBPerson712
RemarkWhen grown on E. coli OP50 animals develop at a normal rate and reach adulthood concomitant with N2 wild-type worms.Paper_evidenceWBPaper00005370
Curator_confirmedWBPerson712
ReferenceWBPaper00037959
WBPaper00033433
WBPaper00005370
WBPaper00035315
WBPaper00035318
WBPaper00055320
WBPaper00056945
WBPaper00061439
WBPaper00065298
WBPaper00066032
MethodSubstitution_allele