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WormBase Tree Display for Transgene: WBTransgene00020486

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Name Class

WBTransgene00020486Public_nameksEx11
Summary[Phsp-16::MEK-1(Ser221Glu)(Ser225Glu); dpy-20(+)]
Synonym[pPD49.83-ceMEK-SSEE, pMH86]
ConstructionConstructWBCnstr00020122
CoinjectionWBCnstr00019441
LaboratoryFK
Construction_summarypPD49.83-ceMEK-SSEE (50 ng/l) was coinjected with 50 ng/l pMH86 dpy-20(+) marker into dpy-20 animals to obtain a transgenic strain, FK137 dpy-20;ksEx11 [pPD49.83-ceMEK-SSEE, pMH86].
Genetic_informationExtrachromosomal
PhenotypeWBPhenotype:0000006Paper_evidenceWBPaper00004381
Curator_confirmedWBPerson2987
RemarkA 30 minute heat shock of strain FK137 at 35 degrees Celsius (to induce expression of an activated form of mek-1 from the ksEx11 transgene) led to cessation of egg layingPaper_evidenceWBPaper00004381
Curator_confirmedWBPerson2987
Caused_by_geneWBGene00003185Paper_evidenceWBPaper00004381
Curator_confirmedWBPerson2987
Phenotype_assayTreatment30 minutes heat shock at 35 degrees CelsiusPaper_evidenceWBPaper00004381
Curator_confirmedWBPerson2987
WBPhenotype:0000747Paper_evidenceWBPaper00004381
Curator_confirmedWBPerson2987
RemarkApproximately 5 hours after a 60 minute heat shock at 33 degrees Celsius (to induce expression of an activated form of mek-1 from the ksEx11 transgene) pharyngeal muscle contraction was suppressed (also see Figure 7)Paper_evidenceWBPaper00004381
Curator_confirmedWBPerson2987
Caused_by_geneWBGene00003185Paper_evidenceWBPaper00004381
Curator_confirmedWBPerson2987
WBPhenotype:0001213Paper_evidenceWBPaper00004381
Curator_confirmedWBPerson2987
RemarkA 30 minute heat shock of strain FK137 at 35 degrees Celsius (to induce expression of an activated form of mek-1 from the ksEx11 transgene) led to cessation of spontaneous movementPaper_evidenceWBPaper00004381
Curator_confirmedWBPerson2987
Caused_by_geneWBGene00003185Paper_evidenceWBPaper00004381
Curator_confirmedWBPerson2987
WBPhenotype:0002221Paper_evidenceWBPaper00004381
Curator_confirmedWBPerson2987
RemarkApproximately 5 hours after a 60 minute heat shock at 33 degrees Celsius (to induce expression of an activated form of mek-1 from the ksEx11 transgene) vacuoles appeared in the pharynx (Figure 6A)Paper_evidenceWBPaper00004381
Curator_confirmedWBPerson2987
Caused_by_geneWBGene00003185Paper_evidenceWBPaper00004381
Curator_confirmedWBPerson2987
Phenotype_assayTreatment60 minute heat shock at 33 degrees CelsiusPaper_evidenceWBPaper00004381
Curator_confirmedWBPerson2987
WBPhenotype:0002222Paper_evidenceWBPaper00004381
Curator_confirmedWBPerson2987
RemarkApproximately 5 hours after a 60 minute heat shock at 33 degrees Celsius (to induce expression of an activated form of mek-1 from the ksEx11 transgene) vacuoles appeared in the uterus lumen (Figure 6B)Paper_evidenceWBPaper00004381
Curator_confirmedWBPerson2987
Caused_by_geneWBGene00003185Paper_evidenceWBPaper00004381
Curator_confirmedWBPerson2987
Associated_withStrainWBStrain00043016
ReferenceWBPaper00004381
SpeciesCaenorhabditis elegans