WormBase Tree Display for Transgene: WBTransgene00018018

WBTransgene00018018 Public_name: WBPaper00042056Ex1
  Summary: [ZYX-1::HA::GFP]
  Synonym: Expr10630_Ex
  Construction: Construct: WBCnstr00017408
  Construction_summary: The pKG106 plasmid was constructed as follows: 1) a 1076 PCR fragment was amplified on pKG83 using the forward primer ctc act gga gaa gtg tacc and the reverse primer tta agc tag cag cgt aat ctg gaa cat cgt atg ggta containing HA-tag encoding sequence and a NheI site. A NcoI and NheI fragment of this PCR product replaced the NcoI-NheI fragment of pKG83. The aim of this step was to suppress the stop codon present in pKG83 after the HA-tag. The resulting plasmid was named pKG98; 2) a 869 GFP coding fragment was amplified on plasmid pPD118.20 using forward primer tta agc tag cat gag taa agg aga aga ac and reverse primer tta agc tag cgc cat gtg taa tcc cagc (both primers containing a NheI site), and inserted into the NheI site of pKG98 resulting in plasmid pKG103; 3) the NruI-ApaI fragment of pKG82 was replaced by the NruI-ApaI fragment from pKG103 (corresponding to 3' genomic sequence including introns 6, 7 and 8 as well as exons 6,7,8, 9 and 10 with the HA- tag and GFP fused in frame at the end of exon 10. -- precise ends.
  Genetic_information: Extrachromosomal
  Used_for: Expr_pattern: Expr10630
  Associated_with: Strain: WBStrain00043918
  Reference: WBPaper00042056
  Species: Caenorhabditis elegans