WormBase Tree Display for Transgene: WBTransgene00001048
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| WBTransgene00001048 | Public_name | muIs61 | |||||
|---|---|---|---|---|---|---|---|
| Summary | [(pKL78) daf16::GFP + rol-6(su1006)] | ||||||
| Synonym | [daf-16::GFP(pKL78) + rol-6(pRF4)] | ||||||
| [daf-16a::GFP + rol-6] | |||||||
| [daf-16a::gfp; rol-6] | |||||||
| Construction | Construct | WBCnstr00001039 | |||||
| Coinjection | WBCnstr00004720 | ||||||
| Integration_method | Gamma_irradiation | ||||||
| Construction_summary | Clone = pKL78. To generate DAF-16a::GFP, the PvuI-StuI fragment from cosmid R13H8, which contains 24.3 kb of the daf-16 genomic sequence, including 6.1 kb upstream of the 5' untranslated region (UTR) and the poly-A signal, was subcloned into plasmid pUC18. A NotI site was created just 5' to the first ATG by PCR amplification of the EcoNI-ApaI fragment, using an inverse PCR strategy. A NotI fragment from plasmid pPD103.87 containing the GFP sequence was inserted into this site to generate an in-frame fusion of GFP immediately upstream of the N-terminus of DAF-16a. One DAF-16a::GFP line (muEx50) was created by injecting DAF-16a::GFP construct at 2 ng/l with 85 ng/l pRF4. muEx50 was subsequently integrated into the chromosome by Gamma-irradiation to generate muIs61. | ||||||
| Genetic_information | Integrated | ||||||
| Map | I | ||||||
| Phenotype | WBPhenotype:0000736 | Paper_evidence | WBPaper00035076 | ||||
| Curator_confirmed | WBPerson2021 | ||||||
| Remark | Seam cell autophagy was increased in muIs61 animals, which over-express DAF-16 | Paper_evidence | WBPaper00035076 | ||||
| Curator_confirmed | WBPerson2021 | ||||||
| Caused_by_gene | WBGene00000912 | Paper_evidence | WBPaper00035076 | ||||
| Curator_confirmed | WBPerson2021 | ||||||
| WBPhenotype:0001014 | Paper_evidence | WBPaper00035076 | |||||
| Curator_confirmed | WBPerson2021 | ||||||
| Remark | Salmonella-infected muIs61 animals lived significantly longer than control N2 wild-type worms | Paper_evidence | WBPaper00035076 | ||||
| Curator_confirmed | WBPerson2021 | ||||||
| Caused_by_gene | WBGene00000912 | Paper_evidence | WBPaper00035076 | ||||
| Curator_confirmed | WBPerson2021 | ||||||
| Used_for | Expr_pattern | Expr3117 | |||||
| Expr11340 | |||||||
| Interactor | WBInteraction000501693 | ||||||
| Associated_with | Strain | WBStrain00004845 | |||||
| WBStrain00004846 | |||||||
| WBStrain00007215 | |||||||
| Reference (21) | |||||||
| Species | Caenorhabditis elegans |
