WormBase Tree Display for Transgene: WBTransgene00001048
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WBTransgene00001048 | Public_name | muIs61 | |
---|---|---|---|
Summary | [(pKL78) daf16::GFP + rol-6(su1006)] | ||
Synonym | [daf-16::GFP(pKL78) + rol-6(pRF4)] | ||
[daf-16a::GFP + rol-6] | |||
[daf-16a::gfp; rol-6] | |||
Construction | Construct | WBCnstr00001039 | |
Coinjection | WBCnstr00004720 | ||
Integration_method | Gamma_irradiation | ||
Construction_summary | Clone = pKL78. To generate DAF-16a::GFP, the PvuI-StuI fragment from cosmid R13H8, which contains 24.3 kb of the daf-16 genomic sequence, including 6.1 kb upstream of the 5' untranslated region (UTR) and the poly-A signal, was subcloned into plasmid pUC18. A NotI site was created just 5' to the first ATG by PCR amplification of the EcoNI-ApaI fragment, using an inverse PCR strategy. A NotI fragment from plasmid pPD103.87 containing the GFP sequence was inserted into this site to generate an in-frame fusion of GFP immediately upstream of the N-terminus of DAF-16a. One DAF-16a::GFP line (muEx50) was created by injecting DAF-16a::GFP construct at 2 ng/l with 85 ng/l pRF4. muEx50 was subsequently integrated into the chromosome by Gamma-irradiation to generate muIs61. | ||
Genetic_information (3) | |||
Used_for | Expr_pattern | Expr3117 | |
Expr11340 | |||
Interactor | WBInteraction000501693 | ||
Associated_with | Strain | WBStrain00004845 | |
WBStrain00004846 | |||
WBStrain00007215 | |||
Reference (21) | |||
Species | Caenorhabditis elegans |