WormBase Tree Display for Transgene: WBTransgene00000399
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| WBTransgene00000399 | Public_name | dhIs59 | |
|---|---|---|---|
| Summary | [Topo::daf-9::GFP + lin-15(+)] | ||
| Synonym | [daf-9::gfp] | ||
| Construction | Construct | WBCnstr00000399 | |
| Construction_summary | daf-9::gfp (10 ng/ul) and lin-15(+) marker (75 ng/ul) were injected into lin-15(n765) animals. dhEx61 and dhEx66 extrachromosomal lines fully rescued daf-9 Mig and Daf-c phenotypes. Integrants dhIs59 and dhIs64 were made from these as described. | ||
| For daf-9::gfp construction, a 9.7 kb genomic fragment containing the daf-9 coding region and 7.4 kb upstream was amplified with primers 5'-GTAGTAATCT GTCCATTGGG GACTACTG-3' and 5'-GGGGTACCTT GATGAGACGA TTTCCGACC-3', and cloned into pCR-Blunt II-TOPO vector (Invitrogen). gfp coding region was then cloned into KpnI-digested daf-9-TOPO. | |||
| translational fusion, a functional gene fusion that rescues mutant phenotypes. --precise ends. | |||
| Genetic_information | Integrated | ||
| Used_for | Expr_pattern | Expr1790 | |
| Expr2916 | |||
| Interactor | WBInteraction000505135 | ||
| Associated_with | Strain | WBStrain00000021 | |
| Reference | WBPaper00005034 | ||
| WBPaper00005054 | |||
| WBPaper00013396 | |||
| WBPaper00036396 | |||
| WBPaper00048673 | |||
| Species | Caenorhabditis elegans |
