WormBase Tree Display for Transgene: WBTransgene00000153
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| WBTransgene00000153 | Public_name | bIs34 | |
|---|---|---|---|
| Summary | [rme-8::GFP + rol-6(su1006)] | ||
| Synonym | [Prme-8::GFP::RME-8; rol-6] | ||
| [RME-8::GFP] | |||
| Construction | Construct | WBCnstr00000153 | |
| Coinjection_other | rol-6(su1006) | ||
| Construction_summary | RME-8::GFP reporters were constructed by inserting GFP into two existing unique restriction enzyme sites in the RME-8 coding region: AgeI in exon 9 or MluI site in exon 21. PCR-amplified GFP (S65C variant from plasmid pPD117.01) engineered with AgeI or MluI sites were inserted into the corresponding unique sites of plasmid F18C12.2FspI. The resulting RME-8 gene fusions (RME-8::GFP/AgeI and RME-8::GFP/MluI) contain the full-length RME-8 protein. --precise ends. | ||
| Genetic_information | Integrated | ||
| Used_for | Expr_pattern | Expr2399 | |
| Expr11494 | |||
| Interactor | WBInteraction000541123 | ||
| WBInteraction000541129 | |||
| WBInteraction000556998 | |||
| Associated_with | Strain | WBStrain00005826 | |
| Reference (29) | |||
| Species | Caenorhabditis elegans |
