WormBase Tree Display for Transgene: WBTransgene00000153

WBTransgene00000153 Public_name: bIs34
  Summary: [rme-8::GFP + rol-6(su1006)]
  Synonym: [Prme-8::GFP::RME-8; rol-6]
    [RME-8::GFP]
  Construction: Construct: WBCnstr00000153
    Coinjection_other: rol-6(su1006)
  Construction_summary: RME-8::GFP reporters were constructed by inserting GFP into two existing unique restriction enzyme sites in the RME-8 coding region: AgeI in exon 9 or MluI site in exon 21. PCR-amplified GFP (S65C variant from plasmid pPD117.01) engineered with AgeI or MluI sites were inserted into the corresponding unique sites of plasmid F18C12.2FspI. The resulting RME-8 gene fusions (RME-8::GFP/AgeI and RME-8::GFP/MluI) contain the full-length RME-8 protein. --precise ends.
  Genetic_information: Integrated
  Used_for: Expr_pattern: Expr2399
      Expr11494
    Interactor: WBInteraction000541123
      WBInteraction000541129
      WBInteraction000556998
  Associated_with: Strain: WBStrain00005826
  Reference: WBPaper00004655
    WBPaper00004810
    WBPaper00006472
    WBPaper00025193
    WBPaper00028972
    WBPaper00029049
    WBPaper00029172
    WBPaper00030865
    WBPaper00031498
    WBPaper00032272
    WBPaper00035216
    WBPaper00035256
    WBPaper00036763
    WBPaper00037899
    WBPaper00038232
    WBPaper00038455
    WBPaper00040913
    WBPaper00041154
    WBPaper00041321
    WBPaper00041681
    WBPaper00042149
    WBPaper00045882
    WBPaper00046372
    WBPaper00049116
    WBPaper00049194
    WBPaper00050040
    WBPaper00051130
    WBPaper00054449
    WBPaper00057149
  Species: Caenorhabditis elegans