WormBase Tree Display for Strain: WBStrain00051783
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| WBStrain00051783 | Status | Live | |
|---|---|---|---|
| Genotype | daf-2(hq363[daf-2::degron::mNeonGreen]) unc-119(ed3) III; hqSi12 IV. | ||
| Public_name | MQD2402 | ||
| Contains | Gene | WBGene00000898 | |
| WBGene00006843 | |||
| Variation | WBVar00145093 | ||
| Properties | Outcrossed | x0 | |
| Mutagen | CRISPR_Cas9 | ||
| CGC_received | 19 Oct 2021 00:00:00 | ||
| Location | CGC | ||
| Remark | hqSi12 [eak-4p::TIR-1:mRuby::unc-54 3' UTR + Cbr-unc-119(+)] IV. Degron and mNeonGreen tag inserted at the 3' end of the endogenous daf-2 gene locus by CRISPR/Cas9 engineering. hqSi12 was generated by replacing the sun-1 promoter and 3' UTR of the ieSi38 insertion (cxTi10882 site) with the eak-4 promoter and unc-54 3'UTR using CRISPR/Cas9. This strain can be used for auxin-inducible degradation (AID) of DAF-2 protein in the XXX cells. hqSi12 previously known as hq388. Reference: Zhang Y, et al. BioRxiv. 2021 Aug 2. 2007.2031.454567. https: | ||
| Made_by: Yan-Ping Zhang, Wen-Hong Zhang | CGC_data_submission | ||
| Species | Caenorhabditis elegans |
