WormBase Tree Display for Strain: WBStrain00051781
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WBStrain00051781 | Status | Live | |
---|---|---|---|
Genotype | hqSi10 II; daf-2(hq363[daf-2::degron::mNeonGreen]) unc-119(ed3) III. | ||
Public_name | MQD2379 | ||
Contains | Gene | WBGene00000898 | |
WBGene00006843 | |||
Variation | WBVar00145093 | ||
Properties | Outcrossed | x0 | |
Mutagen | CRISPR_Cas9 | ||
CGC_received | 19 Oct 2021 00:00:00 | ||
Location | CGC | ||
Remark | hqSi10 [myo-3p::TIR1::mRuby::unc-54 3' UTR + Cbr-unc-119(+)] II. Degron and mNeonGreen tag inserted at the 3' end of the endogenous daf-2 gene locus by CRISPR/Cas9 engineering. hqSi10 was generated by replacing the eft-3 promoter of the ieSi57 insertion (oxTi179 site) with the myo-3 promoter using CRISPR/Cas9. This strain can be used for auxin-inducible degradation (AID) of DAF-2 protein in the body wall muscles. hqSi10 previously known as hq375. Reference: Zhang Y, et al. BioRxiv. 2021 Aug 2. 2007.2031.454567. https: | ||
Made_by: Yan-Ping Zhang, Wen-Hong Zhang | CGC_data_submission | ||
Species | Caenorhabditis elegans |