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WormBase Tree Display for Strain: WBStrain00051227

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Name Class

WBStrain00051227StatusLive
Genotypenola-3(gk5610[loxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + loxP])/+ I.
Public_nameVC4539
ContainsGeneWBGene00003514
WBGene00004496
WBGene00006789
WBGene00007708
VariationWBVar02156455
PropertiesOutcrossedx0
MutagenCRISPR_Cas9
CGC_received31 Aug 2020 00:00:00
LocationCGC
RemarkMade_by: Vancouver KO GroupCGC_data_submission
[NOTE: Please see RG5022 for balanced version of this strain.] Apparent homozygous lethal or sterile deletion as unbalanced heterozygote. Deletion of 433 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break. Pick viable fertile GFP+ animals to maintain. Left flanking sequence: AGCTACTCAGCACGTGCCTATTATCCTCAC. Right flanking sequence: GCTGGTTTGGGAGTGGCGCCGCATGTTGCG. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation.Inferred_automaticallyFrom CGC strain data
SpeciesCaenorhabditis elegans