WormBase Tree Display for Strain: WBStrain00051036
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| WBStrain00051036 | Status | Live | ||
|---|---|---|---|---|
| Genotype | rpa-2(ok1627) rpa-4(iow59[3xFLAG::rpa-4])I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). | |||
| Public_name | SSM410 | |||
| Contains | Gene | WBGene00000254 | ||
| WBGene00019079 | ||||
| WBGene00019767 | ||||
| Variation | WBVar00092835 | |||
| WBVar00143617 | ||||
| WBVar00241187 | ||||
| Rearrangement | hT2 | |||
| Transgene | WBTransgene00001901 | |||
| Properties | Outcrossed | x0 | ||
| Mutagen | CRISPR_Cas9 | |||
| CGC_received | 01 Mar 2021 00:00:00 | |||
| Location | CGC | |||
| Remark | N-terminal 3xFLAG tag inserted into the endogenous rpa-4 locus using Crispr/Cas9. Homozygous sterile deletion balanced by bli-4- and GFP-marked translocation. Heterozygotes are wild-type with pharyngeal GFP signal, and segregate wild-type GFP heterozygotes, arrested hT2 aneuploids, and non-GFP ok1627 homozygotes. Homozygous hT2[bli-4 let-? qIs48] inviable. Pick wild-type GFP and check for correct segregation of progeny to maintain. Generated in rpa-2(ok1627) background. Reference: Hefel et al., Nucleic Acids Res. 2021 Jan 21;gkaa1293. doi: 10.1093/nar/gkaa1293. | Inferred_automatically | From CGC strain data | |
| Made_by: Adam Hefel | CGC_data_submission | |||
| Species | Caenorhabditis elegans |
