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WormBase Tree Display for Strain: WBStrain00037788

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Name Class

WBStrain00037788StatusLive
Genotypealh-12(gk3392) III/hT2[bli-4(e937) let-?(q782) qIs48] (I;III).
Public_nameVC3462
ContainsGeneWBGene00000118
WBGene00000254
VariationWBVar00143617
WBVar00241187
WBVar02125378
RearrangementhT2
TransgeneWBTransgene00001901
PropertiesOutcrossedx1
MutagenUV+TMP
CGC_received07 Mar 2014 00:00:00
LocationCGC
Made_byWBPerson14471
RemarkThis strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.Paper_evidenceWBPaper00041807
Mutagen:UV/TMPCurator_confirmedWBPerson1983
Y69F12A.2. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP heterozygotes, arrested hT2 aneuploids, and possibly non-GFP gk3392 homozygotes (if not Emb; undetermined). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain.External left primer: CGGATCTGTCTGGTGGACTT. External right primer: GTTTCCAGCCGATACGACAT. Internal left primer: GCAACAGCGGATATTGTTGAT. Internal right primer: CAATTTTCACGTCCATGTCCT. Internal WT amplicon: 1413 bp. Deletion size: 709 bp. Deletion left flank: TCCAGGTGGACCATCTCAACGTATTGCCTA. Deletion right flank: ATTACTGGACTTTCCGATGAAGCTAGAGCT.Inferred_automaticallyFrom CGC strain data
SpeciesCaenorhabditis elegans