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WormBase Tree Display for Strain: WBStrain00037371

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Name Class

WBStrain00037371StatusLive
Genotypeptp-2(ok3252)/mIn1 [mIs14 dpy-10(e128)] II.
Public_nameVC2508
ContainsGeneWBGene00001072
WBGene00004214
VariationWBVar00142982
WBVar00094308
RearrangementmIn1
mIn1[dpy-10(e128) mIs14(myo-2::GFP)]
TransgeneWBTransgene00000999
PropertiesOutcrossedx1
MutagenEMS
CGC_received25 Mar 2009 00:00:00
LocationCGC
Made_byWBPerson14471
RemarkThis strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.Paper_evidenceWBPaper00041807
F59G1.5. Homozygous sterile deletion chromosome balanced by GFP- and dpy-10-marked inversion. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP, Dpy bright GFP (mIn1 homozygotes), and non-GFP ok3252 homozygotes (sterile adult). Pick WT dim GFP and check for correct segregation of progeny to maintain. External left primer: CAGTATCTGTCGAAACGCGA. External right primer: CCTGAGAAAATGGGAAGCAA. Internal left primer: CGACGACCAGTTAATGCTGA. Internal right primer: TGATGACGTGGAAGAAGTGC. Internal WT amplicon: 1163 bp. Deletion size: 652 bp. Deletion left flank: GGTCGACGACCAGTTAATGCTGAAAAGAAT. Deletion right flank: TCGTTGTTCATTGTAGTGCTGGAATTGGTA. Insertion Sequence: CG.Inferred_automaticallyFrom CGC strain data
SpeciesCaenorhabditis elegans