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WormBase Tree Display for Strain: WBStrain00037194

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Name Class

WBStrain00037194StatusLive
Genotypeacs-4(ok2872) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III).
Public_nameVC2240
ContainsGeneWBGene00000254
WBGene00018152
VariationWBVar00143617
WBVar00241187
WBVar00093967
RearrangementhT2
hT2[bli-4(e937) let-?(q782) qIs48]
TransgeneWBTransgene00001901
PropertiesOutcrossedx1
MutagenEMS
CGC_received24 Sep 2008 00:00:00
LocationCGC
Made_byWBPerson20180
RemarkThis strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.Paper_evidenceWBPaper00041807
F37C12.7. Homozygous sterile deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ok2872 homozygotes (sterile adult). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: CTGTTTCAGGCAAATTGGGT. External right primer: TTCCTGTGCTCAAGTCGTTG. Internal left primer: ATGTTTGGGAACTCGACAGC. Internal right primer: ATCCTTGAACAACAGGGCAG. Internal WT amplicon: 1170 bp. Deletion size: 335 bp. Deletion left flank: CTGAAGACCCTGATTTATTTCGCTCCTGTG. Deletion right flank: AATTTTTATTGGATTTAAAACTCATTTTAC. Insertion Sequence: CGAAATA.Inferred_automaticallyFrom CGC strain data
SpeciesCaenorhabditis elegans