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WormBase Tree Display for Strain: WBStrain00037025

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Name Class

WBStrain00037025StatusLive
Genotype+/szT1[lon-2(e678)] I; mIs12 II; sec-3(ok2238)/szT1 X.
Public_nameVC2003
ContainsGeneWBGene00003056
WBGene00018703
VariationWBVar00143398
WBVar00093402
RearrangementszT1
szT1[lon-2(e678)]
TransgeneWBTransgene00000997
PropertiesOutcrossedx1
MutagenEMS
CGC_received25 Sep 2014 00:00:00
LocationCGC
Made_byWBPerson20176
RemarkThis strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.Paper_evidenceWBPaper00041807
F52E4.7. Apparent homozygous lethal deletion chromosome balanced by lon-2-marked translocation, and homozygous for unlinked pharyngeal GFP insertion mIs12 (artifact of strain construction). Balanced lethal heterozygotes are WT, and segregate WT, Lon-2 males, arrested szT1 aneuploids, and ok2238 homozygotes (arrest stage/phenotype undetermined). Pick WT and check for correct segregation of progeny to maintain.External left primer: CAATCTTCGAGCCTGGGTAA. External right primer: TACCTTCCAGTCCAGATGCC. Internal left primer: TGAAATGGCGATTTTGATGA. Internal right primer: CATGATATGGCGATGCAAAG. Internal WT amplicon: 2918 bp. Deletion size: 1120 bp. Deletion left flank: TTTCTCCATACTACGTCCTCCGAGACTTGA. Deletion right flank: AATGAAACGATTTCCTCGTTGAGACGTTGC.Inferred_automaticallyFrom CGC strain data
SpeciesCaenorhabditis elegans