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WormBase Tree Display for Strain: WBStrain00036809

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Name Class

WBStrain00036809StatusLive
GenotypeC38H2.2(ok2175) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III).
Public_nameVC1706
ContainsGeneWBGene00000254
WBGene00008019
VariationWBVar00143617
WBVar00241187
WBVar00093346
RearrangementhT2
hT2[bli-4(e937) let-?(q782) qIs48]
TransgeneWBTransgene00001901
PropertiesOutcrossedx1
MutagenEMS
CGC_received25 Feb 2008 00:00:00
LocationCGC
Made_byWBPerson20180
RemarkThis strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.Paper_evidenceWBPaper00041807
C38H2.2. Homozygous sterile deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ok2175 homozygotes (sterile, lays no eggs). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: ATGTGCTGGTGATATGGCAA. External right primer: GAAAAGGCACAGGGTGTGAT. Internal left primer: TCAGACAACCTACGACGCTG. Internal right primer: AGGGTGGAGAACAGTCATGG. Internal WT amplicon: 2946 bp. Deletion size: 767 bp. Deletion left flank: GCGAAGAAGGTTCGCGTCTTCTGTTGGATT. Deletion right flank: GGTAATTTCAGATTCATTGAAGTAGCGCTG.Inferred_automaticallyFrom CGC strain data
SpeciesCaenorhabditis elegans