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WormBase Tree Display for Strain: WBStrain00036753

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Name Class

WBStrain00036753StatusLive
Genotypeunc-120(gk719) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III).
Public_nameVC1633
ContainsGeneWBGene00000254
WBGene00006844
VariationWBVar00146042
WBVar00143617
WBVar00241187
RearrangementhT2
hT2[bli-4(e937) let-?(q782) qIs48]
TransgeneWBTransgene00001901
PropertiesOutcrossedx1
MutagenUV+TMP
CGC_received26 Sep 2007 00:00:00
LocationCGC
Made_byWBPerson20176
RemarkThis strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.Paper_evidenceWBPaper00041807
D1081.2. Homozygous lethal deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP gk719 homozygotes (embryonic or early larval arrest). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: CACTACCTTCACCCCTCCAA. External right primer: CTATAACACGGGACCCCCTT. Internal left primer: GGTCCTTCCATTCCCATCTT. Internal right primer: GGCTGACATAACATCGCTCA. Internal WT amplicon: 2150 bp. Deletion size: 972 bp. Deletion left flank: ATGTTTCTAAAATTTATCTGCATTTTCATA. Deletion right flank: AAATATCCTGACTCACCTATTTAGTTGCGG.Inferred_automaticallyFrom CGC strain data
Mutagen:UV/TMPCurator_confirmedWBPerson1983
SpeciesCaenorhabditis elegans