WormBase Tree Display for Strain: WBStrain00036614
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| WBStrain00036614 | Status | Live | ||
|---|---|---|---|---|
| Genotype | npp-15(ok1954) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). | |||
| Public_name | VC1459 | |||
| Contains | Gene | WBGene00000254 | ||
| WBGene00003801 | ||||
| Variation | WBVar00143617 | |||
| WBVar00241187 | ||||
| WBVar00093149 | ||||
| Rearrangement | hT2 | |||
| hT2[bli-4(e937) let-?(q782) qIs48] | ||||
| Transgene | WBTransgene00001901 | |||
| Properties | Outcrossed | x1 | ||
| Mutagen | EMS | |||
| CGC_received | 14 Nov 2006 00:00:00 | |||
| Location | CGC | |||
| Made_by | WBPerson20176 | |||
| Remark | This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. | Paper_evidence | WBPaper00041807 | |
| C29E4.4. Homozygous lethal deletion chromosome balanced by bli-4-, let-?- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ok1954 homozygotes (probable early larval arrest). Homozygous hT2[bli-4 let-? qIs48] normally inviable; occasional recombinants are seen (Bli, bright GFP). Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: AGGCTTCCAATTTGACATCG. External right primer: CCACTCGACTTCCACCTTGT. Internal left primer: CATTCATCTTTCGCTGGGTT. Internal right primer: GTCTGCTGGCTTTCCAAGAG. Internal WT amplicon: 3083 bp. Deletion size: 1597 bp. Deletion left flank: ATACCCGGCACTGGTGAAAGAGGCCTTTCT. Deletion right flank: ATGGCAGAGTCGGAAGAGGAATTAAAGGCG. | Inferred_automatically | From CGC strain data | ||
| Species | Caenorhabditis elegans |
