WormBase Tree Display for Strain: WBStrain00000001
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WBStrain00000001 | Status | Live | |||||||
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Genotype | Caenorhabditis elegans wild isolate. | ||||||||
Public_name | N2 | ||||||||
Contains | Clone (15355) | ||||||||
Disease_info | Models_disease | DOID:1289 | |||||||
DOID:1826 | |||||||||
DOID:9351 | |||||||||
DOID:10652 | |||||||||
DOID:0050589 | |||||||||
DOID:0070333 | |||||||||
DOID:0080933 | |||||||||
Models_disease_in_annotation (16) | |||||||||
Properties | CGC_received | 01 Jan 1993 00:00:00 | |||||||
09 Jul 1993 00:00:00 | |||||||||
13 May 2019 00:00:00 | |||||||||
Phenotype (53) | |||||||||
Phenotype_not_observed | WBPhenotype:0000039 | Paper_evidence | WBPaper00059661 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Our results indicate that obeticholic acid does not have a consistent beneficial effect on lifespan in any of the C. elegans or C. briggsae strains tested at the concentrations used. This conclusion is based upon two biological replicates at each concentration performed in one lab, resulting in an average of 104 individuals measured per strain and concentration, and should be considered preliminary. | Paper_evidence | WBPaper00059661 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Affected_by | Molecule | WBMol:00007887 | Paper_evidence | WBPaper00059661 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000048 | Paper_evidence | WBPaper00064301 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | "We found no difference in hatching rates on a high-glucose diet compared to control diet: the number of viable larval-stage animals relative to the number of embryos laid was ~100% on both diets (Fig.1G). This suggests that a high-glucose diet does not reduce fertility by disrupting embryonic development." | Paper_evidence | WBPaper00064301 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Affected_by | Molecule | WBMol:00007746 | Paper_evidence | WBPaper00064301 | |||||
Curator_confirmed | WBPerson712 | ||||||||
EQ_annotations | Life_stage | WBls:0000041 | PATO:0000460 | Paper_evidence | WBPaper00064301 | ||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000061 | Paper_evidence | WBPaper00031694 | |||||||
WBPaper00058993 | |||||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Of deprenyl, reserpine, and haloperidol, only reserpine could extend C. elegans lifespan by 31% at 25 deg C upon chronic treatment from embryo till death. At 20C, no significant lifespan extension was observed. Reserpine-treated worms had a mean life span of 17 days, in contrast to untreated worms with a mean life span of 13 days. | Paper_evidence | WBPaper00031694 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
-GPA does not extend lifespan at the concentrations tested. This conclusion is based on one biological replicate per concentration and, as such, could be considered preliminary. | Paper_evidence | WBPaper00058993 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Image | WBPicture0000014943 | Paper_evidence | WBPaper00058993 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Affected_by | Molecule | WBMol:00004621 | Paper_evidence | WBPaper00031694 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBMol:00007871 | Paper_evidence | WBPaper00058993 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000072 | Paper_evidence | WBPaper00060382 | |||||||
WBPaper00065095 | |||||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | We report that lifelong exposure to 0.025mM or 1mM Auxin does not significantly affect any of the objectively quantified phenotypic features in either N2 or PD1074 wild-type strains (Figure 1D-G). Body length and width were no different among animals exposed to 0 to 1mM auxin exposure. | Paper_evidence | WBPaper00060382 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
"... we measured adult worm length and width at 96 hours of age. Interestingly, Parafilm-wrapped plates did not yield significantly longer or wider worms compared to non-wrapped plates (Fig. 1A-B, Mann-Whitney U test, Worm Width U = 117.0, p > 0.05; Worm Length U = 120.0, p > 0.05). However, this is not surprising because at 96 hours (Day 4) animals would have reached the point of maturation where the rate of growth in size has significantly decreased (Croll et al., 1977)." | Paper_evidence | WBPaper00065095 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Affected_by | Molecule | WBMol:00007857 | Paper_evidence | WBPaper00060382 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBMol:00001774 | Paper_evidence | WBPaper00065095 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000154 | Paper_evidence | WBPaper00056577 | |||||||
WBPaper00064301 | |||||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | "High-glucose diet in L4 alone does not affect progeny production. Animals on a high-glucose diet only during the L4 stage (gray open points) produced the same number of offspring as those on a control diet throughout development and adulthood (filled gray points)." "Exposure to a high-glucose diet only in the L4 stage and during the L4 to adult transition had no effect on the number of offspring produced (Fig. 1B). These results indicate that the decreased progeny production in response to a high-glucose diet is due to processes that occur during adulthood, and exposure to high-glucose diet during development does not affect this facet of fertility." | Paper_evidence | WBPaper00064301 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Affected_by | Molecule | WBMol:00007746 | Paper_evidence | WBPaper00064301 | |||||
Curator_confirmed | WBPerson712 | ||||||||
EQ_annotations | Life_stage | WBls:0000109 | PATO:0000460 | Paper_evidence | WBPaper00064301 | ||||
Curator_confirmed | WBPerson712 | ||||||||
WBls:0000040 | PATO:0000460 | Paper_evidence | WBPaper00064301 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBls:0000064 | PATO:0000460 | Paper_evidence | WBPaper00064301 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Phenotype_assay | Treatment | Animals were age-synchronized by hypochlorite treatment (Stiernagle, 2006) and grown on plates seeded with Escherichia coli OP50. Ten L4 animals were manually transferred to individual plates seeded with S. epidermidis. Plates were completely covered by bacteria to prevent animals from avoiding the bacteria. Adults were daily transferred to new plates and the number of eggs laid on each plate was counted every 24 hours until no more eggs were laid. Statistical analyses were performed using repeated measures ANOVA and Tukey's post-hoc test.S. epidermidis strain ATCC 49134. | Paper_evidence | WBPaper00056577 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000227 | Paper_evidence | WBPaper00061691 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000481 | Paper_evidence | WBPaper00060627 | |||||||
WBPaper00065019 | |||||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | We examined wild type young N2 adults in the presence of undiluted chemical repellent, Boundary, and analyzed chemotaxis behavior over 0-60 minutes after the cat repellent cue was added to the avoidance assay plate (Fig. 1A, Schematic Diagram of assay). Interestingly, upon examining wild type adults, we repulsion was evident over a 60 minute period after the odor cues we re-added to the plate (Avoidance Index: ~0.5 index, Fig. 1A-1B). To further characterize the response, we also examined wild type animals at diluted concentrations of the repellent, including, undiluted tested against 1:2 and 1:4 dilutions (Fig. 1C). These concentrations were compared to undiluted repellent each time using wild type N2 worms (Fig. 1C). Dilutions of repellent resulted in some response at 1:2 dilutions, and significantly reduced response at 1:4 dilutions across 60 minutes (Fig. 1C). To examine the avoidance of the cat sensed odor repellent (Boundary-contains methyl nonyl ketone/2-undecanone and other chemicals, Petco), chemotactic avoidance assays were performed essentially as previously described in Troemel et al., 1997. | Paper_evidence | WBPaper00060627 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Methyl nonyl ketone (2-undecanone) has been previously shown to be an animal and insect repellent (Innocent et al., 2008). Interestingly, this is also repulsive to wild type worms, as wild type N2 worms avoided this cue (Fig. 1D). Suggesting, Boundary and one of its chemical constituents, methyl nonyl ketone (2-undecanone) are repulsive to wild type C. elegans. | Paper_evidence | WBPaper00060627 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Across thirty strains, including an N2 strain, there was a significant effect of Argentine ant extract on chemotaxis behavior (ANOVA, F(29) = 2.3364, p = 0.001). Four strains showed a significantly different response when compared to N2, including ED3052 (t(84) = 2.862, p = 0.039), JU2519 (t(84) =3.091, p = 0.026), JU830 (t(84) = 3.309, p = 0.020), and NIC277 (t(84) = 3.956, p = 0.004). | Paper_evidence | WBPaper00065019 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Affected_by | Molecule | WBMol:00007923 | Paper_evidence | WBPaper00060627 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBMol:00007924 | Paper_evidence | WBPaper00060627 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
WBMol:00008001 | Paper_evidence | WBPaper00065019 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000523 | Paper_evidence | WBPaper00038487 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | N2 animals traveling perpendicular to the NaCl attractant followed a path that curved with a bias toward the attractant; that curving bias decreases over time. | Paper_evidence | WBPaper00038487 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Affected_by | Molecule | WBMol:00003571 | Paper_evidence | WBPaper00038487 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000643 | Paper_evidence | WBPaper00056078 | |||||||
WBPaper00060382 | |||||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | The speed of displacement of the worm and the wave amplitude of its shape in free-movement were evaluated after 3 h of drug exposure. 7(3-bromopropoxy)-chrysin did not elicit significant difference compared to the control | Paper_evidence | WBPaper00056078 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
We report that lifelong exposure to 0.025mM or 1mM Auxin does not significantly affect any of the objectively quantified phenotypic features in either N2 or PD1074 wild-type strains (Figure 1D-G). Locomotion speed and bias were no different among animals exposed to 0 to 1mM auxin exposure. | Paper_evidence | WBPaper00060382 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Affected_by | Molecule | WBMol:00007860 | Paper_evidence | WBPaper00056078 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBMol:00007857 | Paper_evidence | WBPaper00060382 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000778 | Paper_evidence | WBPaper00032342 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Between 20 and 48 hr, the fluorescence levels of GFP expressing bacteria decreased ~1.2% per hour, which was the same rate observed for RC301 animals on a similar lawn of bacteria. | Paper_evidence | WBPaper00032342 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Phenotype_assay | Treatment | Animals were grown on a lawn of E. coli constitutively expressing GFP, in which the fluorescent intensity in the outer 40% of the lawn (border) is 35% brighter than in the inner 60% (center), suggesting differences in bacterial density. | Paper_evidence | WBPaper00032342 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000812 | Paper_evidence | WBPaper00061728 | |||||||
Curator_confirmed | WBPerson557 | ||||||||
Remark | Authors found that incubating L1 larvae for 3-5 hours in a solution of 100 M latrunculin A did not result in significant primordial germ line disorganization and the fluorescence levels of the mNG::ANI-1 marker at the proto-rachis remained unchanged compared to control. | Paper_evidence | WBPaper00061728 | ||||||
Curator_confirmed | WBPerson557 | ||||||||
Authors found that incubating L1 larvae for 3-5 hours in a solution of 100 M latrunculin A did not result in significant primordial germ line disorganization and the fluorescence levels of the LifeAct::mKate2 marker (marking F-actin) at the proto-rachis remained unchanged compared to control. | Paper_evidence | WBPaper00061728 | |||||||
Curator_confirmed | WBPerson557 | ||||||||
Affected_by | Molecule | WBMol:00005353 | Paper_evidence | WBPaper00061728 | |||||
Curator_confirmed | WBPerson557 | ||||||||
EQ_annotations | Life_stage | WBls:0000024 | PATO:0000460 | Paper_evidence | WBPaper00061728 | ||||
Curator_confirmed | WBPerson557 | ||||||||
Phenotype_assay | Treatment | Treated N2 L1 larvae with the actin depolymerizing drug latrunculin A (100 micromolar) and scored primordial germ line organization. | Paper_evidence | WBPaper00061728 | |||||
Curator_confirmed | WBPerson557 | ||||||||
Temperature | 20 | Paper_evidence | WBPaper00061728 | ||||||
Curator_confirmed | WBPerson557 | ||||||||
Genotype | mNG::ANI-1 | Paper_evidence | WBPaper00061728 | ||||||
Curator_confirmed | WBPerson557 | ||||||||
LifeAct::mKate2 | Paper_evidence | WBPaper00061728 | |||||||
Curator_confirmed | WBPerson557 | ||||||||
WBPhenotype:0001434 | Paper_evidence | WBPaper00059397 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Affected_by | Molecule | WBMol:00007882 | Paper_evidence | WBPaper00059397 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBMol:00001807 | Paper_evidence | WBPaper00059397 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
WBMol:00007881 | Paper_evidence | WBPaper00059397 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
WBMol:00001657 | Paper_evidence | WBPaper00059397 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0001623 | Paper_evidence | WBPaper00061691 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0001703 | Paper_evidence | WBPaper00060382 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | We report that lifelong exposure to 0.025mM or 1mM Auxin does not significantly affect any of the objectively quantified phenotypic features in either N2 or PD1074 wild-type strains (Figure 1D-G). Degree of kink in body posture were no different among animals exposed to 0 to 1mM auxin exposure. | Paper_evidence | WBPaper00060382 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Affected_by | Molecule | WBMol:00007857 | Paper_evidence | WBPaper00060382 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0001715 | Paper_evidence | WBPaper00038487 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Animals regulated pirouette-mediated reversals as a function of direction near food. | Paper_evidence | WBPaper00038487 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0001871 | Paper_evidence | WBPaper00040161 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Wild-type worms show identical lifespan on NGM and FUdR plates. | There was no effect of FUdR on wild-type lifespan across a range of concentrations. | Paper_evidence | WBPaper00040161 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0001983 | Paper_evidence | WBPaper00060628 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Wild type animals generally leave an E.coli OP50 food patch across 0 - 60 minutes infrequently, approximately 3 out of 20-25 worms left an E. coli OP50 food patch across at least the first 1 hour (Fig. 1A, Schematic of behavior assay). | Paper_evidence | WBPaper00060628 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0002079 | Paper_evidence | WBPaper00064301 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | "As we observed for total fertility, high-glucose diet only during L4 and the L4 to adult transition did not affect reproductive timing (Figs. 1D and 1F)." | Paper_evidence | WBPaper00064301 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Affected_by | Molecule | WBMol:00007746 | Paper_evidence | WBPaper00064301 | |||||
Curator_confirmed | WBPerson712 | ||||||||
EQ_annotations | Life_stage | WBls:0000040 | PATO:0000460 | Paper_evidence | WBPaper00064301 | ||||
Curator_confirmed | WBPerson712 | ||||||||
WBls:0000109 | PATO:0000460 | Paper_evidence | WBPaper00064301 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0002320 | Paper_evidence | WBPaper00060382 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | We report that lifelong exposure to 0.025mM or 1mM Auxin does not significantly affect any of the objectively quantified phenotypic features in either N2 or PD1074 wild-type strains (Figure 1D-G). Reversal probability, speed and duration were no different from no to 1mM auxin exposure. | Paper_evidence | WBPaper00060382 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Affected_by | Molecule | WBMol:00007857 | Paper_evidence | WBPaper00060382 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0002545 | Paper_evidence | WBPaper00060628 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | We tested wild type N2 worms on an additional food patch, Comamonas sp (Fig. 1D). Wild type animals left with similar rates when compared to E. coli OP50 (Fig. 1B, and 1D). | Paper_evidence | WBPaper00060628 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0004022 | Paper_evidence | WBPaper00056078 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | The speed of displacement of the worm and the wave amplitude of its shape in free-movement were evaluated after 3 h of drug exposure. chrysin at 100 uM reduced the speed movement without changes on the wave amplitude of the nematode | Paper_evidence | WBPaper00056078 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Affected_by | Molecule | WBMol:00004034 | Paper_evidence | WBPaper00056078 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0004030 | Paper_evidence | WBPaper00061691 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Location | CGC | ||||||||
Remark (30) | |||||||||
Reference (99) | |||||||||
Species | Caenorhabditis elegans | ||||||||
Wild_isolate | |||||||||
Isolation | GPS | 51.46 | -2.6 | ||||||
Place | Bristol, Great Britain | ||||||||
Landscape | Urban_garden | ||||||||
Substrate | mushroom_compost | ||||||||
Sampled_by | W. Nicholas | ||||||||
Isolated_by | WBPerson2877 | ||||||||
Date | 01 JAN 1951 00:00:00 |