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WormBase Tree Display for Strain: WBStrain00000001

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Name Class

WBStrain00000001StatusLive
GenotypeCaenorhabditis elegans wild isolate.
Public_nameN2
ContainsClone (15355)
Disease_infoModels_diseaseDOID:1289
DOID:1826
DOID:9351
DOID:10652
DOID:0050589
DOID:0070333
DOID:0080933
Models_disease_in_annotation (16)
PropertiesCGC_received01 Jan 1993 00:00:00
09 Jul 1993 00:00:00
13 May 2019 00:00:00
Phenotype (53)
Phenotype_not_observedWBPhenotype:0000039Paper_evidenceWBPaper00059661
Curator_confirmedWBPerson712
RemarkOur results indicate that obeticholic acid does not have a consistent beneficial effect on lifespan in any of the C. elegans or C. briggsae strains tested at the concentrations used. This conclusion is based upon two biological replicates at each concentration performed in one lab, resulting in an average of 104 individuals measured per strain and concentration, and should be considered preliminary.Paper_evidenceWBPaper00059661
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00007887Paper_evidenceWBPaper00059661
Curator_confirmedWBPerson712
WBPhenotype:0000048Paper_evidenceWBPaper00064301
Curator_confirmedWBPerson712
Remark"We found no difference in hatching rates on a high-glucose diet compared to control diet: the number of viable larval-stage animals relative to the number of embryos laid was ~100% on both diets (Fig.1G). This suggests that a high-glucose diet does not reduce fertility by disrupting embryonic development."Paper_evidenceWBPaper00064301
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00007746Paper_evidenceWBPaper00064301
Curator_confirmedWBPerson712
EQ_annotationsLife_stageWBls:0000041PATO:0000460Paper_evidenceWBPaper00064301
Curator_confirmedWBPerson712
WBPhenotype:0000061Paper_evidenceWBPaper00031694
WBPaper00058993
Curator_confirmedWBPerson712
RemarkOf deprenyl, reserpine, and haloperidol, only reserpine could extend C. elegans lifespan by 31% at 25 deg C upon chronic treatment from embryo till death. At 20C, no significant lifespan extension was observed. Reserpine-treated worms had a mean life span of 17 days, in contrast to untreated worms with a mean life span of 13 days.Paper_evidenceWBPaper00031694
Curator_confirmedWBPerson712
-GPA does not extend lifespan at the concentrations tested. This conclusion is based on one biological replicate per concentration and, as such, could be considered preliminary.Paper_evidenceWBPaper00058993
Curator_confirmedWBPerson712
ImageWBPicture0000014943Paper_evidenceWBPaper00058993
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00004621Paper_evidenceWBPaper00031694
Curator_confirmedWBPerson712
WBMol:00007871Paper_evidenceWBPaper00058993
Curator_confirmedWBPerson712
WBPhenotype:0000072Paper_evidenceWBPaper00060382
WBPaper00065095
Curator_confirmedWBPerson712
RemarkWe report that lifelong exposure to 0.025mM or 1mM Auxin does not significantly affect any of the objectively quantified phenotypic features in either N2 or PD1074 wild-type strains (Figure 1D-G). Body length and width were no different among animals exposed to 0 to 1mM auxin exposure.Paper_evidenceWBPaper00060382
Curator_confirmedWBPerson712
"... we measured adult worm length and width at 96 hours of age. Interestingly, Parafilm-wrapped plates did not yield significantly longer or wider worms compared to non-wrapped plates (Fig. 1A-B, Mann-Whitney U test, Worm Width U = 117.0, p > 0.05; Worm Length U = 120.0, p > 0.05). However, this is not surprising because at 96 hours (Day 4) animals would have reached the point of maturation where the rate of growth in size has significantly decreased (Croll et al., 1977)."Paper_evidenceWBPaper00065095
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00007857Paper_evidenceWBPaper00060382
Curator_confirmedWBPerson712
WBMol:00001774Paper_evidenceWBPaper00065095
Curator_confirmedWBPerson712
WBPhenotype:0000154Paper_evidenceWBPaper00056577
WBPaper00064301
Curator_confirmedWBPerson712
Remark"High-glucose diet in L4 alone does not affect progeny production. Animals on a high-glucose diet only during the L4 stage (gray open points) produced the same number of offspring as those on a control diet throughout development and adulthood (filled gray points)." "Exposure to a high-glucose diet only in the L4 stage and during the L4 to adult transition had no effect on the number of offspring produced (Fig. 1B). These results indicate that the decreased progeny production in response to a high-glucose diet is due to processes that occur during adulthood, and exposure to high-glucose diet during development does not affect this facet of fertility."Paper_evidenceWBPaper00064301
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00007746Paper_evidenceWBPaper00064301
Curator_confirmedWBPerson712
EQ_annotationsLife_stageWBls:0000109PATO:0000460Paper_evidenceWBPaper00064301
Curator_confirmedWBPerson712
WBls:0000040PATO:0000460Paper_evidenceWBPaper00064301
Curator_confirmedWBPerson712
WBls:0000064PATO:0000460Paper_evidenceWBPaper00064301
Curator_confirmedWBPerson712
Phenotype_assayTreatmentAnimals were age-synchronized by hypochlorite treatment (Stiernagle, 2006) and grown on plates seeded with Escherichia coli OP50. Ten L4 animals were manually transferred to individual plates seeded with S. epidermidis. Plates were completely covered by bacteria to prevent animals from avoiding the bacteria. Adults were daily transferred to new plates and the number of eggs laid on each plate was counted every 24 hours until no more eggs were laid. Statistical analyses were performed using repeated measures ANOVA and Tukey's post-hoc test.S. epidermidis strain ATCC 49134.Paper_evidenceWBPaper00056577
Curator_confirmedWBPerson712
WBPhenotype:0000227Paper_evidenceWBPaper00061691
Curator_confirmedWBPerson712
WBPhenotype:0000481Paper_evidenceWBPaper00060627
WBPaper00065019
Curator_confirmedWBPerson712
RemarkWe examined wild type young N2 adults in the presence of undiluted chemical repellent, Boundary, and analyzed chemotaxis behavior over 0-60 minutes after the cat repellent cue was added to the avoidance assay plate (Fig. 1A, Schematic Diagram of assay). Interestingly, upon examining wild type adults, we repulsion was evident over a 60 minute period after the odor cues we re-added to the plate (Avoidance Index: ~0.5 index, Fig. 1A-1B). To further characterize the response, we also examined wild type animals at diluted concentrations of the repellent, including, undiluted tested against 1:2 and 1:4 dilutions (Fig. 1C). These concentrations were compared to undiluted repellent each time using wild type N2 worms (Fig. 1C). Dilutions of repellent resulted in some response at 1:2 dilutions, and significantly reduced response at 1:4 dilutions across 60 minutes (Fig. 1C). To examine the avoidance of the cat sensed odor repellent (Boundary-contains methyl nonyl ketone/2-undecanone and other chemicals, Petco), chemotactic avoidance assays were performed essentially as previously described in Troemel et al., 1997.Paper_evidenceWBPaper00060627
Curator_confirmedWBPerson712
Methyl nonyl ketone (2-undecanone) has been previously shown to be an animal and insect repellent (Innocent et al., 2008). Interestingly, this is also repulsive to wild type worms, as wild type N2 worms avoided this cue (Fig. 1D). Suggesting, Boundary and one of its chemical constituents, methyl nonyl ketone (2-undecanone) are repulsive to wild type C. elegans.Paper_evidenceWBPaper00060627
Curator_confirmedWBPerson712
Across thirty strains, including an N2 strain, there was a significant effect of Argentine ant extract on chemotaxis behavior (ANOVA, F(29) = 2.3364, p = 0.001). Four strains showed a significantly different response when compared to N2, including ED3052 (t(84) = 2.862, p = 0.039), JU2519 (t(84) =3.091, p = 0.026), JU830 (t(84) = 3.309, p = 0.020), and NIC277 (t(84) = 3.956, p = 0.004).Paper_evidenceWBPaper00065019
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00007923Paper_evidenceWBPaper00060627
Curator_confirmedWBPerson712
WBMol:00007924Paper_evidenceWBPaper00060627
Curator_confirmedWBPerson712
WBMol:00008001Paper_evidenceWBPaper00065019
Curator_confirmedWBPerson712
WBPhenotype:0000523Paper_evidenceWBPaper00038487
Curator_confirmedWBPerson712
RemarkN2 animals traveling perpendicular to the NaCl attractant followed a path that curved with a bias toward the attractant; that curving bias decreases over time.Paper_evidenceWBPaper00038487
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00003571Paper_evidenceWBPaper00038487
Curator_confirmedWBPerson712
WBPhenotype:0000643Paper_evidenceWBPaper00056078
WBPaper00060382
Curator_confirmedWBPerson712
RemarkThe speed of displacement of the worm and the wave amplitude of its shape in free-movement were evaluated after 3 h of drug exposure. 7(3-bromopropoxy)-chrysin did not elicit significant difference compared to the controlPaper_evidenceWBPaper00056078
Curator_confirmedWBPerson712
We report that lifelong exposure to 0.025mM or 1mM Auxin does not significantly affect any of the objectively quantified phenotypic features in either N2 or PD1074 wild-type strains (Figure 1D-G). Locomotion speed and bias were no different among animals exposed to 0 to 1mM auxin exposure.Paper_evidenceWBPaper00060382
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00007860Paper_evidenceWBPaper00056078
Curator_confirmedWBPerson712
WBMol:00007857Paper_evidenceWBPaper00060382
Curator_confirmedWBPerson712
WBPhenotype:0000778Paper_evidenceWBPaper00032342
Curator_confirmedWBPerson712
RemarkBetween 20 and 48 hr, the fluorescence levels of GFP expressing bacteria decreased ~1.2% per hour, which was the same rate observed for RC301 animals on a similar lawn of bacteria.Paper_evidenceWBPaper00032342
Curator_confirmedWBPerson712
Phenotype_assayTreatmentAnimals were grown on a lawn of E. coli constitutively expressing GFP, in which the fluorescent intensity in the outer 40% of the lawn (border) is 35% brighter than in the inner 60% (center), suggesting differences in bacterial density.Paper_evidenceWBPaper00032342
Curator_confirmedWBPerson712
WBPhenotype:0000812Paper_evidenceWBPaper00061728
Curator_confirmedWBPerson557
RemarkAuthors found that incubating L1 larvae for 3-5 hours in a solution of 100 M latrunculin A did not result in significant primordial germ line disorganization and the fluorescence levels of the mNG::ANI-1 marker at the proto-rachis remained unchanged compared to control.Paper_evidenceWBPaper00061728
Curator_confirmedWBPerson557
Authors found that incubating L1 larvae for 3-5 hours in a solution of 100 M latrunculin A did not result in significant primordial germ line disorganization and the fluorescence levels of the LifeAct::mKate2 marker (marking F-actin) at the proto-rachis remained unchanged compared to control.Paper_evidenceWBPaper00061728
Curator_confirmedWBPerson557
Affected_byMoleculeWBMol:00005353Paper_evidenceWBPaper00061728
Curator_confirmedWBPerson557
EQ_annotationsLife_stageWBls:0000024PATO:0000460Paper_evidenceWBPaper00061728
Curator_confirmedWBPerson557
Phenotype_assayTreatmentTreated N2 L1 larvae with the actin depolymerizing drug latrunculin A (100 micromolar) and scored primordial germ line organization.Paper_evidenceWBPaper00061728
Curator_confirmedWBPerson557
Temperature20Paper_evidenceWBPaper00061728
Curator_confirmedWBPerson557
GenotypemNG::ANI-1Paper_evidenceWBPaper00061728
Curator_confirmedWBPerson557
LifeAct::mKate2Paper_evidenceWBPaper00061728
Curator_confirmedWBPerson557
WBPhenotype:0001434Paper_evidenceWBPaper00059397
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00007882Paper_evidenceWBPaper00059397
Curator_confirmedWBPerson712
WBMol:00001807Paper_evidenceWBPaper00059397
Curator_confirmedWBPerson712
WBMol:00007881Paper_evidenceWBPaper00059397
Curator_confirmedWBPerson712
WBMol:00001657Paper_evidenceWBPaper00059397
Curator_confirmedWBPerson712
WBPhenotype:0001623Paper_evidenceWBPaper00061691
Curator_confirmedWBPerson712
WBPhenotype:0001703Paper_evidenceWBPaper00060382
Curator_confirmedWBPerson712
RemarkWe report that lifelong exposure to 0.025mM or 1mM Auxin does not significantly affect any of the objectively quantified phenotypic features in either N2 or PD1074 wild-type strains (Figure 1D-G). Degree of kink in body posture were no different among animals exposed to 0 to 1mM auxin exposure.Paper_evidenceWBPaper00060382
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00007857Paper_evidenceWBPaper00060382
Curator_confirmedWBPerson712
WBPhenotype:0001715Paper_evidenceWBPaper00038487
Curator_confirmedWBPerson712
RemarkAnimals regulated pirouette-mediated reversals as a function of direction near food.Paper_evidenceWBPaper00038487
Curator_confirmedWBPerson712
WBPhenotype:0001871Paper_evidenceWBPaper00040161
Curator_confirmedWBPerson712
RemarkWild-type worms show identical lifespan on NGM and FUdR plates. | There was no effect of FUdR on wild-type lifespan across a range of concentrations.Paper_evidenceWBPaper00040161
Curator_confirmedWBPerson712
WBPhenotype:0001983Paper_evidenceWBPaper00060628
Curator_confirmedWBPerson712
RemarkWild type animals generally leave an E.coli OP50 food patch across 0 - 60 minutes infrequently, approximately 3 out of 20-25 worms left an E. coli OP50 food patch across at least the first 1 hour (Fig. 1A, Schematic of behavior assay).Paper_evidenceWBPaper00060628
Curator_confirmedWBPerson712
WBPhenotype:0002079Paper_evidenceWBPaper00064301
Curator_confirmedWBPerson712
Remark"As we observed for total fertility, high-glucose diet only during L4 and the L4 to adult transition did not affect reproductive timing (Figs. 1D and 1F)."Paper_evidenceWBPaper00064301
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00007746Paper_evidenceWBPaper00064301
Curator_confirmedWBPerson712
EQ_annotationsLife_stageWBls:0000040PATO:0000460Paper_evidenceWBPaper00064301
Curator_confirmedWBPerson712
WBls:0000109PATO:0000460Paper_evidenceWBPaper00064301
Curator_confirmedWBPerson712
WBPhenotype:0002320Paper_evidenceWBPaper00060382
Curator_confirmedWBPerson712
RemarkWe report that lifelong exposure to 0.025mM or 1mM Auxin does not significantly affect any of the objectively quantified phenotypic features in either N2 or PD1074 wild-type strains (Figure 1D-G). Reversal probability, speed and duration were no different from no to 1mM auxin exposure.Paper_evidenceWBPaper00060382
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00007857Paper_evidenceWBPaper00060382
Curator_confirmedWBPerson712
WBPhenotype:0002545Paper_evidenceWBPaper00060628
Curator_confirmedWBPerson712
RemarkWe tested wild type N2 worms on an additional food patch, Comamonas sp (Fig. 1D). Wild type animals left with similar rates when compared to E. coli OP50 (Fig. 1B, and 1D).Paper_evidenceWBPaper00060628
Curator_confirmedWBPerson712
WBPhenotype:0004022Paper_evidenceWBPaper00056078
Curator_confirmedWBPerson712
RemarkThe speed of displacement of the worm and the wave amplitude of its shape in free-movement were evaluated after 3 h of drug exposure. chrysin at 100 uM reduced the speed movement without changes on the wave amplitude of the nematodePaper_evidenceWBPaper00056078
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00004034Paper_evidenceWBPaper00056078
Curator_confirmedWBPerson712
WBPhenotype:0004030Paper_evidenceWBPaper00061691
Curator_confirmedWBPerson712
LocationCGC
Remark (30)
Reference (99)
SpeciesCaenorhabditis elegans
Wild_isolate
IsolationGPS51.46-2.6
PlaceBristol, Great Britain
LandscapeUrban_garden
Substratemushroom_compost
Sampled_byW. Nicholas
Isolated_byWBPerson2877
Date01 JAN 1951 00:00:00