WormBase Tree Display for Strain: WBStrain00000001
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WBStrain00000001 | Status | Live | |||||||
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Genotype | Caenorhabditis elegans wild isolate. | ||||||||
Public_name | N2 | ||||||||
Contains | Clone (15355) | ||||||||
Disease_info | Models_disease | DOID:1289 | |||||||
DOID:1826 | |||||||||
DOID:9351 | |||||||||
DOID:10652 | |||||||||
DOID:0050589 | |||||||||
DOID:0070333 | |||||||||
DOID:0080933 | |||||||||
Models_disease_in_annotation (16) | |||||||||
Properties | CGC_received | 01 Jan 1993 00:00:00 | |||||||
09 Jul 1993 00:00:00 | |||||||||
13 May 2019 00:00:00 | |||||||||
Phenotype (53) | |||||||||
Phenotype_not_observed | WBPhenotype:0000039 | Paper_evidence | WBPaper00059661 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Our results indicate that obeticholic acid does not have a consistent beneficial effect on lifespan in any of the C. elegans or C. briggsae strains tested at the concentrations used. This conclusion is based upon two biological replicates at each concentration performed in one lab, resulting in an average of 104 individuals measured per strain and concentration, and should be considered preliminary. | Paper_evidence | WBPaper00059661 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Affected_by | Molecule | WBMol:00007887 | Paper_evidence | WBPaper00059661 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000048 | Paper_evidence | WBPaper00064301 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | "We found no difference in hatching rates on a high-glucose diet compared to control diet: the number of viable larval-stage animals relative to the number of embryos laid was ~100% on both diets (Fig.1G). This suggests that a high-glucose diet does not reduce fertility by disrupting embryonic development." | Paper_evidence | WBPaper00064301 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Affected_by | Molecule | WBMol:00007746 | Paper_evidence | WBPaper00064301 | |||||
Curator_confirmed | WBPerson712 | ||||||||
EQ_annotations | Life_stage | WBls:0000041 | PATO:0000460 | Paper_evidence | WBPaper00064301 | ||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000061 | Paper_evidence | WBPaper00031694 | |||||||
WBPaper00058993 | |||||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark (2) | |||||||||
Image | WBPicture0000014943 | Paper_evidence | WBPaper00058993 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Affected_by | Molecule | WBMol:00004621 | Paper_evidence | WBPaper00031694 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBMol:00007871 | Paper_evidence | WBPaper00058993 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000072 | Paper_evidence | WBPaper00060382 | |||||||
WBPaper00065095 | |||||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark (2) | |||||||||
Affected_by | Molecule | WBMol:00007857 | Paper_evidence | WBPaper00060382 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBMol:00001774 | Paper_evidence | WBPaper00065095 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000154 | Paper_evidence | WBPaper00056577 | |||||||
WBPaper00064301 | |||||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | "High-glucose diet in L4 alone does not affect progeny production. Animals on a high-glucose diet only during the L4 stage (gray open points) produced the same number of offspring as those on a control diet throughout development and adulthood (filled gray points)." "Exposure to a high-glucose diet only in the L4 stage and during the L4 to adult transition had no effect on the number of offspring produced (Fig. 1B). These results indicate that the decreased progeny production in response to a high-glucose diet is due to processes that occur during adulthood, and exposure to high-glucose diet during development does not affect this facet of fertility." | Paper_evidence | WBPaper00064301 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Affected_by | Molecule | WBMol:00007746 | Paper_evidence | WBPaper00064301 | |||||
Curator_confirmed | WBPerson712 | ||||||||
EQ_annotations | Life_stage | WBls:0000109 | PATO:0000460 | Paper_evidence | WBPaper00064301 | ||||
Curator_confirmed | WBPerson712 | ||||||||
WBls:0000040 | PATO:0000460 | Paper_evidence | WBPaper00064301 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBls:0000064 | PATO:0000460 | Paper_evidence | WBPaper00064301 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Phenotype_assay | Treatment | Animals were age-synchronized by hypochlorite treatment (Stiernagle, 2006) and grown on plates seeded with Escherichia coli OP50. Ten L4 animals were manually transferred to individual plates seeded with S. epidermidis. Plates were completely covered by bacteria to prevent animals from avoiding the bacteria. Adults were daily transferred to new plates and the number of eggs laid on each plate was counted every 24 hours until no more eggs were laid. Statistical analyses were performed using repeated measures ANOVA and Tukey's post-hoc test.S. epidermidis strain ATCC 49134. | Paper_evidence | WBPaper00056577 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000227 | Paper_evidence | WBPaper00061691 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000481 | Paper_evidence | WBPaper00060627 | |||||||
WBPaper00065019 | |||||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark (3) | |||||||||
Affected_by | Molecule | WBMol:00007923 | Paper_evidence | WBPaper00060627 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBMol:00007924 | Paper_evidence | WBPaper00060627 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
WBMol:00008001 | Paper_evidence | WBPaper00065019 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000523 | Paper_evidence | WBPaper00038487 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | N2 animals traveling perpendicular to the NaCl attractant followed a path that curved with a bias toward the attractant; that curving bias decreases over time. | Paper_evidence | WBPaper00038487 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Affected_by | Molecule | WBMol:00003571 | Paper_evidence | WBPaper00038487 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000643 | Paper_evidence | WBPaper00056078 | |||||||
WBPaper00060382 | |||||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark (2) | |||||||||
Affected_by | Molecule | WBMol:00007860 | Paper_evidence | WBPaper00056078 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBMol:00007857 | Paper_evidence | WBPaper00060382 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000778 | Paper_evidence | WBPaper00032342 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Between 20 and 48 hr, the fluorescence levels of GFP expressing bacteria decreased ~1.2% per hour, which was the same rate observed for RC301 animals on a similar lawn of bacteria. | Paper_evidence | WBPaper00032342 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Phenotype_assay | Treatment | Animals were grown on a lawn of E. coli constitutively expressing GFP, in which the fluorescent intensity in the outer 40% of the lawn (border) is 35% brighter than in the inner 60% (center), suggesting differences in bacterial density. | Paper_evidence | WBPaper00032342 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000812 | Paper_evidence | WBPaper00061728 | |||||||
Curator_confirmed | WBPerson557 | ||||||||
Remark (2) | |||||||||
Affected_by | Molecule | WBMol:00005353 | Paper_evidence | WBPaper00061728 | |||||
Curator_confirmed | WBPerson557 | ||||||||
EQ_annotations | Life_stage | WBls:0000024 | PATO:0000460 | Paper_evidence | WBPaper00061728 | ||||
Curator_confirmed | WBPerson557 | ||||||||
Phenotype_assay | Treatment | Treated N2 L1 larvae with the actin depolymerizing drug latrunculin A (100 micromolar) and scored primordial germ line organization. | Paper_evidence | WBPaper00061728 | |||||
Curator_confirmed | WBPerson557 | ||||||||
Temperature | 20 | Paper_evidence | WBPaper00061728 | ||||||
Curator_confirmed | WBPerson557 | ||||||||
Genotype | mNG::ANI-1 | Paper_evidence | WBPaper00061728 | ||||||
Curator_confirmed | WBPerson557 | ||||||||
LifeAct::mKate2 | Paper_evidence | WBPaper00061728 | |||||||
Curator_confirmed | WBPerson557 | ||||||||
WBPhenotype:0001434 | Paper_evidence | WBPaper00059397 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Affected_by | Molecule | WBMol:00007882 | Paper_evidence | WBPaper00059397 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBMol:00001807 | Paper_evidence | WBPaper00059397 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
WBMol:00007881 | Paper_evidence | WBPaper00059397 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
WBMol:00001657 | Paper_evidence | WBPaper00059397 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0001623 | Paper_evidence | WBPaper00061691 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0001703 | Paper_evidence | WBPaper00060382 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | We report that lifelong exposure to 0.025mM or 1mM Auxin does not significantly affect any of the objectively quantified phenotypic features in either N2 or PD1074 wild-type strains (Figure 1D-G). Degree of kink in body posture were no different among animals exposed to 0 to 1mM auxin exposure. | Paper_evidence | WBPaper00060382 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Affected_by | Molecule | WBMol:00007857 | Paper_evidence | WBPaper00060382 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0001715 | Paper_evidence | WBPaper00038487 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Animals regulated pirouette-mediated reversals as a function of direction near food. | Paper_evidence | WBPaper00038487 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0001871 | Paper_evidence | WBPaper00040161 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Wild-type worms show identical lifespan on NGM and FUdR plates. | There was no effect of FUdR on wild-type lifespan across a range of concentrations. | Paper_evidence | WBPaper00040161 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0001983 | Paper_evidence | WBPaper00060628 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Wild type animals generally leave an E.coli OP50 food patch across 0 - 60 minutes infrequently, approximately 3 out of 20-25 worms left an E. coli OP50 food patch across at least the first 1 hour (Fig. 1A, Schematic of behavior assay). | Paper_evidence | WBPaper00060628 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0002079 | Paper_evidence | WBPaper00064301 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | "As we observed for total fertility, high-glucose diet only during L4 and the L4 to adult transition did not affect reproductive timing (Figs. 1D and 1F)." | Paper_evidence | WBPaper00064301 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Affected_by | Molecule | WBMol:00007746 | Paper_evidence | WBPaper00064301 | |||||
Curator_confirmed | WBPerson712 | ||||||||
EQ_annotations | Life_stage | WBls:0000040 | PATO:0000460 | Paper_evidence | WBPaper00064301 | ||||
Curator_confirmed | WBPerson712 | ||||||||
WBls:0000109 | PATO:0000460 | Paper_evidence | WBPaper00064301 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0002320 | Paper_evidence | WBPaper00060382 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | We report that lifelong exposure to 0.025mM or 1mM Auxin does not significantly affect any of the objectively quantified phenotypic features in either N2 or PD1074 wild-type strains (Figure 1D-G). Reversal probability, speed and duration were no different from no to 1mM auxin exposure. | Paper_evidence | WBPaper00060382 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Affected_by | Molecule | WBMol:00007857 | Paper_evidence | WBPaper00060382 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0002545 | Paper_evidence | WBPaper00060628 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | We tested wild type N2 worms on an additional food patch, Comamonas sp (Fig. 1D). Wild type animals left with similar rates when compared to E. coli OP50 (Fig. 1B, and 1D). | Paper_evidence | WBPaper00060628 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0004022 | Paper_evidence | WBPaper00056078 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | The speed of displacement of the worm and the wave amplitude of its shape in free-movement were evaluated after 3 h of drug exposure. chrysin at 100 uM reduced the speed movement without changes on the wave amplitude of the nematode | Paper_evidence | WBPaper00056078 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Affected_by | Molecule | WBMol:00004034 | Paper_evidence | WBPaper00056078 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0004030 | Paper_evidence | WBPaper00061691 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Location | CGC | ||||||||
Remark (30) | |||||||||
Reference (99) | |||||||||
Species | Caenorhabditis elegans | ||||||||
Wild_isolate | |||||||||
Isolation | GPS | 51.46 | -2.6 | ||||||
Place | Bristol, Great Britain | ||||||||
Landscape | Urban_garden | ||||||||
Substrate | mushroom_compost | ||||||||
Sampled_by | W. Nicholas | ||||||||
Isolated_by | WBPerson2877 | ||||||||
Date | 01 JAN 1951 00:00:00 |