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WormBase Tree Display for Strain: WBStrain00000001

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Name Class

WBStrain00000001StatusLive
GenotypeCaenorhabditis elegans wild isolate.
Public_nameN2
ContainsClone (15355)
Disease_infoModels_diseaseDOID:1289
DOID:1826
DOID:9351
DOID:10652
DOID:0050589
DOID:0070333
DOID:0080933
Models_disease_in_annotation (16)
PropertiesCGC_received01 Jan 1993 00:00:00
09 Jul 1993 00:00:00
13 May 2019 00:00:00
PhenotypeWBPhenotype:0000018Paper_evidenceWBPaper00038239
WBPaper00050383
Curator_confirmedWBPerson712
RemarkEach application of 5-HT elicited a robust excitatory response in pharyngeal preparations, which were exposed to three consecutive applications of 5-HT separated by a 2-min interval. This response to 5-HT can be inhibited by emodepside.Paper_evidenceWBPaper00038239
Curator_confirmedWBPerson712
Adults were reared and tested in M9 medium plus 5 mM trehalose, which has previously been shown to extend lifespan and increase pump frequency late in life in C. elegans (Honda et al., 2010). Using an alternate electrophysiological readout of pumping, electrophayrngeograms (EPGs), we examined pump frequency at earlier time points than previously reported. Pumps were stimulated with 10mM 5HT in M9, recorded as EPGs in a microfluidic device, and analyzed using NemAnalysis software (NemaMetrix). Pump frequency in trehalose-treated animals was significantly higher in 11-day adults than in controls of the same age that were reared in parallel and tested on the same day (p<0.01; 1-tailed Mann-Whitney U-test; n = 5-8 worms at each age in each condition). This finding confirms, by an independent method, previously reported data (Honda et al., 2010).Paper_evidenceWBPaper00050383
Curator_confirmedWBPerson712
ImageWBPicture0000013534Paper_evidenceWBPaper00050383
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00004929Paper_evidenceWBPaper00038239
Curator_confirmedWBPerson712
WBMol:00004935Paper_evidenceWBPaper00050383
Curator_confirmedWBPerson712
WBPhenotype:0000061Paper_evidenceWBPaper00031694
WBPaper00026929
WBPaper00046225
WBPaper00061801
WBPaper00065348
WBPaper00047022
Curator_confirmedWBPerson712
WBPerson557
RemarkOf deprenyl, reserpine, and haloperidol, only reserpine could extend C. elegans lifespan by 31% at 25 deg C upon chronic treatment from embryo till death. At 20C, no significant lifespan extension was observed. Reserpine-treated worms had a mean life span of 17 days, in contrast to untreated worms with a mean life span of 13 days.Paper_evidenceWBPaper00031694
Curator_confirmedWBPerson712
Resveratrol extended C. elegans life span in a dosage- dependent manner, increasing both the mean and maximum life span of wild-type worms. A nearly 18% increase in mean life span was obtained with 1mM resveratrol.Paper_evidenceWBPaper00026929
Curator_confirmedWBPerson712
Direct treatment of WT worms with the OEA analog prolonged life span (Fig. 4G and table S1).Paper_evidenceWBPaper00046225
Curator_confirmedWBPerson712
"diuron does not exert robust positive effects on lifespan across three species of Caenorhabditis nematodes (Fig. 1).C. elegans strain N2 showed a weakly significant, non-robust increase in mean lifespan at 100 M and 200 M."Paper_evidenceWBPaper00061801
Curator_confirmedWBPerson712
NR treatment only increased mean lifespan by 3%, while BHB increased mean lifespan by 21%. Combination treatment increased mean lifespan by 18% that was not significantly different than the addition of BHB alone. | Lifespan experiments were then performed using the same treatments, but first administered at the L1 larval stage (Figure 1B) instead of the L4 stage. The results are summarized in Experiment 2 in Table 1. NR increased mean lifespan by 20% consistent with previous results of NR-mediated longevity (Mouchiroud et al., 2013) and suggesting the requirement of the full non-blunted UPRmt activated during larval development for the anti-aging effects. | Treatment with NR only during larval development increased mean lifespan by 28%,Paper_evidenceWBPaper00065348
Curator_confirmedWBPerson712
BHB increased mean lifespan by 21%. Combination treatment increased mean lifespan by 18% that was not significantly different than the addition of BHB alone.Paper_evidenceWBPaper00065348
Curator_confirmedWBPerson712
Otophylloside B (Ot B), a C-21 steroidal glycoside, extended adult lifespan in C. elegans (N2 strain).Paper_evidenceWBPaper00047022
Curator_confirmedWBPerson557
Affected_byMoleculeWBMol:00002955Paper_evidenceWBPaper00031694
Curator_confirmedWBPerson712
WBMol:00004910Paper_evidenceWBPaper00026929
Curator_confirmedWBPerson712
WBMol:00005442Paper_evidenceWBPaper00046225
Curator_confirmedWBPerson712
WBMol:00003951Paper_evidenceWBPaper00061801
Curator_confirmedWBPerson712
WBMol:00008014Paper_evidenceWBPaper00065348
Curator_confirmedWBPerson712
WBMol:00008015Paper_evidenceWBPaper00065348
Curator_confirmedWBPerson712
WBMol:00007846Paper_evidenceWBPaper00047022
Curator_confirmedWBPerson557
EQ_annotationsLife_stageWBls:0000106PATO:0000460Paper_evidenceWBPaper00065348
Curator_confirmedWBPerson712
WBls:0000041PATO:0000460Paper_evidenceWBPaper00065348
Curator_confirmedWBPerson712
WBls:0000023PATO:0000460Paper_evidenceWBPaper00065348
Curator_confirmedWBPerson712
Temperature_sensitiveHeat_sensitive25Paper_evidenceWBPaper00031694
Curator_confirmedWBPerson712
Phenotype_assayTreatmentAnimals grown on 50uM Otophylloside (Ot B). Ot B is a C-21 steroidal glycoside isolated from Qingyangshen (Cynanchum otophyllum schneid), a herbaceous plant used in traditional Chinese medicine.Paper_evidenceWBPaper00047022
Curator_confirmedWBPerson557
WBPhenotype:0000083Paper_evidenceWBPaper00050460
Curator_confirmedWBPerson712
RemarkLarge amounts of Cu (greater than 200 uM) resulted in growth arrest at the L3 stage, likely due to Cu toxicity. BCS treatment resulted in ~ 40% reduction in total Cu content as compared to no supplementation. No significant changes in Fe and Zn content were observed in worms treated with Cu or BCS (Supplemental Fig. S1A-B).Paper_evidenceWBPaper00050460
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00002485Paper_evidenceWBPaper00050460
Curator_confirmedWBPerson712
WBMol:00004203Paper_evidenceWBPaper00050460
Curator_confirmedWBPerson712
EQ_annotationsLife_stageWBls:0000108PATO:0000297Paper_evidenceWBPaper00050460
Curator_confirmedWBPerson712
GO_termGO:0060003PATO:0000460Paper_evidenceWBPaper00050460
Curator_confirmedWBPerson712
Molecule_affectedWBMol:00007840PATO:0000460Paper_evidenceWBPaper00050460
Curator_confirmedWBPerson712
WBPhenotype:0000119Paper_evidenceWBPaper00036476
Curator_confirmedWBPerson712
RemarkNuclear SBP-1::GFP expression remains high in fasted nematodes treated with the sirtuin inhibitors nicotinamide and sirtinol.Paper_evidenceWBPaper00036476
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00002704Paper_evidenceWBPaper00036476
Curator_confirmedWBPerson712
WBMol:00001943Paper_evidenceWBPaper00036476
Curator_confirmedWBPerson712
WBPhenotype:0000130Paper_evidenceWBPaper00049337
Curator_confirmedWBPerson712
RemarkWe found that the addition of 0.1 mM quinine enhanced dauer formation in response to ascr#3 in wild-type animals.Paper_evidenceWBPaper00049337
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00003904Paper_evidenceWBPaper00049337
Curator_confirmedWBPerson712
Phenotype_assayGenotypekyIs128[str-3p::GFP]Paper_evidenceWBPaper00049337
Curator_confirmedWBPerson712
WBPhenotype:0000135Paper_evidenceWBPaper00057200
Curator_confirmedWBPerson712
RemarkAs shown in Figure 1B, pgph-1, T23F2.4, and cyp-14A5 were induced by 24 h exposure to 250 mM NaCl; nine biological replicates were measured for each condition.Paper_evidenceWBPaper00057200
Curator_confirmedWBPerson712
ImageWBPicture0000014925Paper_evidenceWBPaper00057200
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00003571Paper_evidenceWBPaper00057200
Curator_confirmedWBPerson712
WBPhenotype:0000140Paper_evidenceWBPaper00041209
Curator_confirmedWBPerson712
RemarkCo-exposure to UVC (10 J/m2) and ethidium bromide (5 mg/ml) further exacerbates L3 arrest compared with UVC (asterisks) or chemical (hash) exposure alone at every time point.Paper_evidenceWBPaper00041209
Curator_confirmedWBPerson712
3-MA exposure alone did not result in significant larval arrest (data not shown); however, 3-MA exposure did exacerbate UVC-induced L3 arrest (Figure 7).Paper_evidenceWBPaper00041209
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00005361Paper_evidenceWBPaper00041209
Curator_confirmedWBPerson712
WBMol:00003513Paper_evidenceWBPaper00041209
Curator_confirmedWBPerson712
EQ_annotationsLife_stageWBls:0000035PATO:0000460Paper_evidenceWBPaper00041209
Curator_confirmedWBPerson712
Phenotype_assayTreatmentUVC (10 J/m2)Paper_evidenceWBPaper00041209
Curator_confirmedWBPerson712
N2 nematodes were exposed to 10mM 3-MA following the third UVC exposure and placement on food.Paper_evidenceWBPaper00041209
Curator_confirmedWBPerson712
WBPhenotype:0000146Paper_evidenceWBPaper00061928
Curator_confirmedWBPerson712
Remark"We found that significantly fewer males exposed to moderate temperature stress can successfully mate and that the small number of males in the population that do successfully mate produce significantly fewer viable cross progeny than non-stressed controls." "JU1171, LKC34, and N2 males were less likely to mate successfully and produced significantly fewer viable cross progeny at 27C."Paper_evidenceWBPaper00061928
Curator_confirmedWBPerson712
EQ_annotationsLife_stageWBls:0000056PATO:0000460Paper_evidenceWBPaper00061928
Curator_confirmedWBPerson712
Phenotype_assayTemperature27 deg CPaper_evidenceWBPaper00061928
Curator_confirmedWBPerson712
WBPhenotype:0000154Paper_evidenceWBPaper00056577
WBPaper00064301
Curator_confirmedWBPerson712
Remark"Exposure to high-glucose diet in adulthood reduces progeny production. Animals on a high-glucose diet only during development (purple) produced the same number of offspring as those on a control diet (Cntl) in both development and adulthood (gray). Animals on a high-glucose diet during adulthood (blue and orange) had significantly fewer offspring, independent of developmental diet (****p < 0.0001)." "Animals fed high-glucose diet in adulthood had decreased progeny production (as in A), whether they were exposed to control diet (open orange points) or high-glucose diet (filled orange points) in L4."Paper_evidenceWBPaper00064301
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00007746Paper_evidenceWBPaper00064301
Curator_confirmedWBPerson712
EQ_annotationsLife_stageWBls:0000041PATO:0000460Paper_evidenceWBPaper00064301
Curator_confirmedWBPerson712
Phenotype_assayTreatmentAnimals were age-synchronized by hypochlorite treatment (Stiernagle, 2006) and grown on plates seeded with Escherichia coli OP50. Ten L4 animals were manually transferred to individual plates seeded with S. marcescens. Plates were completely covered by bacteria to prevent animals from avoiding the bacteria. Adults were daily transferred to new plates and the number of eggs laid on each plate was counted every 24 hours until no more eggs were laid. Statistical analyses were performed using repeated measures ANOVA and Tukey's post-hoc test. S. marcescens from Carolina Biological Supply Company.Paper_evidenceWBPaper00056577
Curator_confirmedWBPerson712
WBPhenotype:0000349Paper_evidenceWBPaper00038239
Curator_confirmedWBPerson712
Remark100nM emodepside elicited a flaccid paralysis, particularly apparent in the anterior of the worm, and a decrease in amplitude of the sinusoidal body shape.Paper_evidenceWBPaper00038239
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00004597Paper_evidenceWBPaper00038239
Curator_confirmedWBPerson712
WBPhenotype:0000351Paper_evidenceWBPaper00059998
Curator_confirmedWBPerson712
Remark"...we examine the hatching rate of C. elegans wild-type (N2) embryos in response to acute TCS exposure (see Methods ). As shown in Fig. 1B-1D, TCS caused pronounced shrinkage of the inner cell mass of embryos in a dose-dependent fashion." 0.2-1 mM triclosan. These effects are ameliorated by co-treatment with Tween-20.Paper_evidenceWBPaper00059998
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00000656Paper_evidenceWBPaper00059998
Curator_confirmedWBPerson712
WBMol:00007891Paper_evidenceWBPaper00059998
Curator_confirmedWBPerson712
WBPhenotype:0000481Paper_evidenceWBPaper00059397
Curator_confirmedWBPerson712
RemarkThe two stereoisomers of carvone were more repellant than solvent control (solvent vs. (R)-(-)-carvone, p<0.0001; solvent vs. (S)-(+)-carvone, p=4.010-6) and (R)-(-)-carvone seemed to elicit stronger responses than (S)-(+)-carvone (p=9.210-6):Paper_evidenceWBPaper00059397
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00007883Paper_evidenceWBPaper00059397
Curator_confirmedWBPerson712
WBMol:00007880Paper_evidenceWBPaper00059397
Curator_confirmedWBPerson712
WBPhenotype:0000518Paper_evidenceWBPaper00050460
Curator_confirmedWBPerson712
RemarkWorm growth showed a biphasic curve over dietary Cu levels (Fig. 1A). C. elegans displayed maximal growth in the low &mu;M range of supplemental Cu and impaired growth at either end of the Cu spectrum, 100 &mu;M BCS and 150 &mu;M Cu, with smaller brood size and delayed development. Monitoring of animal development revealed that at optimal Cu (~2 &mu;M), worms became gravid adults in 3 days, while most animals grown at high or low Cu conditions only reached the L3 to young adult stage at this time point (Fig. 1B)Paper_evidenceWBPaper00050460
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00007840Paper_evidenceWBPaper00050460
Curator_confirmedWBPerson712
EQ_annotationsGO_termGO:0006878PATO:0000460Paper_evidenceWBPaper00050460
Curator_confirmedWBPerson712
WBPhenotype:0000631Paper_evidenceWBPaper00040857
Curator_confirmedWBPerson712
RemarkTreatment of wild-type animals with LiCl substantially reduced the fraction of isothermal behavior, altered the localization pattern of SNB-1::GFP, and shortens the body length of animals.Paper_evidenceWBPaper00040857
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00003608Paper_evidenceWBPaper00040857
Curator_confirmedWBPerson712
WBPhenotype:0000643Paper_evidenceWBPaper00038239
WBPaper00065095
Curator_confirmedWBPerson712
RemarkLocomotion of C. elegans after both short-term (3-h) and long-term (24-h) exposure to rottlerin was assessed. Short-term (3-h) exposure to rottlerin did not inhibit locomotion of wild-type. Long-term (24-h) exposure to rottlerin affected locomotion of wild-type worms.Paper_evidenceWBPaper00038239
Curator_confirmedWBPerson712
Analysis of the speed of worms before taps (defined as the locomotor speed of animals between 3s 0.1s prior to the tap stimulus), yielded a significant difference between the two conditions for the first, third, and fourth taps (Fig. 1E; Mann-Whitney U test, Tap 1 U = 297.0, p <0.001; Tap 2 U = 142.0; p > 0.05; Tap 3 U = 61.0, p < 0.01; Tap 4 U = 11.0, p < 0.001). There was also a significant difference in speed between the second and third taps during the interval 0.1s 1s after the taps occurred (Fig. 1F; Mann-Whitney U test, Tap 1 U = 124.0, p > 0.05; Tap 2 U = 80.0, p < 0.01; Tap 3 U = 39.0, p < 0.001).Paper_evidenceWBPaper00065095
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00003190Paper_evidenceWBPaper00038239
Curator_confirmedWBPerson712
WBMol:00001774Paper_evidenceWBPaper00065095
Curator_confirmedWBPerson712
WBPhenotype:0000661Paper_evidenceWBPaper00003187
WBPaper00032342
Curator_confirmedWBPerson712
RemarkAnimals are solitary foragers and disperse evenly across the bacterial lawn.Paper_evidenceWBPaper00003187
Curator_confirmedWBPerson712
Phenotype_assayTreatmentAnimals were assayed on a bacterial lawn.Paper_evidenceWBPaper00003187
Curator_confirmedWBPerson712
Animals were grown on a lawn of E. coli constitutively expressing GFP, in which the fluorescent intensity in the outer 40% of the lawn (border) is w35% brighter than in the inner 60% (center), suggesting differences in bacterial density.Paper_evidenceWBPaper00032342
Curator_confirmedWBPerson712
WBPhenotype:0000662Paper_evidenceWBPaper00003187
Curator_confirmedWBPerson712
RemarkFood induced a roughly 2-fold inhibition of movement in solitary but not social strains.Paper_evidenceWBPaper00003187
Curator_confirmedWBPerson712
WBPhenotype:0000679Paper_evidenceWBPaper00047022
Curator_confirmedWBPerson557
RemarkOtophylloside B (Ot B), a C-21 steroidal glycoside, caused DAF16::GFP nuclear localization in N2, while in control N2 animals DAF16::GFP was localized to the cytoplasm.Paper_evidenceWBPaper00047022
Curator_confirmedWBPerson557
Affected_byMoleculeWBMol:00007846Paper_evidenceWBPaper00047022
Curator_confirmedWBPerson557
Phenotype_assayTreatmentAnimals grown on 50uM Otophylloside (Ot B). Ot B is a C-21 steroidal glycoside isolated from Qingyangshen (Cynanchum otophyllum schneid), a herbaceous plant used in traditional Chinese medicine.Paper_evidenceWBPaper00047022
Curator_confirmedWBPerson557
GenotypeDAF16::GFPPaper_evidenceWBPaper00047022
Curator_confirmedWBPerson557
WBPhenotype:0000739Paper_evidenceWBPaper00029334
Curator_confirmedWBPerson712
RemarkDifferent life stages of N2 or glp-1 nematodes exposed to 0, 100, or 200 J/m2 UVC exhibited marked differences in susceptibilityto induction of DNA damage (Figure 2), with starved L1 larvae the most and 1-day-old N2 adults the least susceptible.Paper_evidenceWBPaper00029334
Curator_confirmedWBPerson712
WBPhenotype:0000770Paper_evidenceWBPaper00059998
Curator_confirmedWBPerson712
Remark"...we examine the hatching rate of C. elegans wild-type (N2) embryos in response to acute TCS exposure (see Methods ). As shown in Fig. 1B-1D, TCS caused pronounced shrinkage of the inner cell mass of embryos in a dose-dependent fashion." 0.2-1 mM triclosan. These effects are ameliorated by co-treatment with Tween-20.Paper_evidenceWBPaper00059998
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00000656Paper_evidenceWBPaper00059998
Curator_confirmedWBPerson712
WBMol:00007891Paper_evidenceWBPaper00059998
Curator_confirmedWBPerson712
WBPhenotype:0000863Paper_evidenceWBPaper00061928
Curator_confirmedWBPerson712
Remark"We found that significantly fewer males exposed to moderate temperature stress can successfully mate and that the small number of males in the population that do successfully mate produce significantly fewer viable cross progeny than non-stressed controls." "JU1171, LKC34, and N2 males were less likely to mate successfully and produced significantly fewer viable cross progeny at 27C."Paper_evidenceWBPaper00061928
Curator_confirmedWBPerson712
EQ_annotationsLife_stageWBls:0000056PATO:0000460Paper_evidenceWBPaper00061928
Curator_confirmedWBPerson712
Phenotype_assayTemperature27 deg CPaper_evidenceWBPaper00061928
Curator_confirmedWBPerson712
WBPhenotype:0000962Paper_evidenceWBPaper00038206
Curator_confirmedWBPerson712
RemarkWild-type animals grown on 1mM colchicine generally suffered a greater reduction in GFP fluorescence than animals with putative loss-of-function mutations (in mec-7 or mec-12).Paper_evidenceWBPaper00038206
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00003637Paper_evidenceWBPaper00038206
Curator_confirmedWBPerson712
Phenotype_assayTreatmentAnimals were grown for multiple generations on plates containing 1 mM colchicine.Paper_evidenceWBPaper00038206
Curator_confirmedWBPerson712
GenotypeuIs43, uIs44(Pmec-18::praja::gfp)Paper_evidenceWBPaper00038206
Curator_confirmedWBPerson712
WBPhenotype:0001012Paper_evidenceWBPaper00040740
Curator_confirmedWBPerson712
RemarkS. avermitilis significantly reduced the brood size and induced uncoordinated movement in N2 animals.Paper_evidenceWBPaper00040740
Curator_confirmedWBPerson712
WBPhenotype:0001065Paper_evidenceWBPaper00056577
Curator_confirmedWBPerson712
Phenotype_assayTreatmentAnimals were age-synchronized by hypochlorite treatment (Stiernagle, 2006) and grown on plates seeded with Escherichia coli OP50. Ten L4 animals were manually transferred to individual plates seeded with S. marcescens. Plates were completely covered by bacteria to prevent animals from avoiding the bacteria. Adults were daily transferred to new plates and the number of eggs laid on each plate was counted every 24 hours until no more eggs were laid. Statistical analyses were performed using repeated measures ANOVA and Tukey's post-hoc test. S. marcescens from Carolina Biological Supply Company.Paper_evidenceWBPaper00056577
Curator_confirmedWBPerson712
Animals were age-synchronized by hypochlorite treatment (Stiernagle, 2006) and grown on plates seeded with Escherichia coli OP50. Ten L4 animals were manually transferred to individual plates seeded with S. epidermidis. Plates were completely covered by bacteria to prevent animals from avoiding the bacteria. Adults were daily transferred to new plates and the number of eggs laid on each plate was counted every 24 hours until no more eggs were laid. Statistical analyses were performed using repeated measures ANOVA and Tukey's post-hoc test.S. epidermidis strain ATCC 49134.Paper_evidenceWBPaper00056577
Curator_confirmedWBPerson712
WBPhenotype:0001101Paper_evidenceWBPaper00060230
Curator_confirmedWBPerson712
Remarklipopolysaccharide (LPS) extracted from S. enterica stimulates gravid adults to lay more eggs in LPS-containing solution than those in M9 buffer only. Figure 1 shows that the worms that were exposed to 0.1 mg/ml LPS for 1 hour laid an average of 5.57 +/- 0.57 eggs (+/- one standard error; n = 101), which was significantly different than the response of the worms exposed to M9 buffer (1.87 +/- 0.25 eggs; n = 112). Exogenous serotonin at 1 mg/ml in M9 resulted in the laying of 8.40 +/- 0.60 eggs in one hour (n = 111 compared to the worms in M9). The magnitude of LPS-stimulated egg laying was not comparable to that of the serotonin one (p = 0.0005, indicating significant difference).Paper_evidenceWBPaper00060230
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00007894Paper_evidenceWBPaper00060230
Curator_confirmedWBPerson712
WBPhenotype:0001171Paper_evidenceWBPaper00065348
Curator_confirmedWBPerson712
RemarkLifespan experiments were then performed using the same treatments, but first administered at the L1 larval stage (Figure 1B) instead of the L4 stage...Surprisingly, administration of 20 mM BHB decreased lifespan by 40% suggesting larval toxicity. When NR and BHB were administered together starting at the L1 stage, there was a 22% decrease in mean lifespan.Paper_evidenceWBPaper00065348
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00008015Paper_evidenceWBPaper00065348
Curator_confirmedWBPerson712
EQ_annotationsLife_stageWBls:0000106PATO:0000460Paper_evidenceWBPaper00065348
Curator_confirmedWBPerson712
WBls:0000023PATO:0000460Paper_evidenceWBPaper00065348
Curator_confirmedWBPerson712
WBPhenotype:0001213Paper_evidenceWBPaper00056078
Curator_confirmedWBPerson712
RemarkThe speed of displacement of the worm and the wave amplitude of its shape in free-movement were evaluated after 3 h of drug exposure. chrysin at 100 uM reduced the speed movement without changes on the wave amplitude of the nematodePaper_evidenceWBPaper00056078
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00004034Paper_evidenceWBPaper00056078
Curator_confirmedWBPerson712
WBPhenotype:0001236Paper_evidenceWBPaper00038115
Curator_confirmedWBPerson712
RemarkLevels of trx-1 are increased in wild type dauer larvae when compared to L2/L3 wild type animals, as assayed by the average relative intensity of fluorescence of ofEx379[Ptrx-1::GFP; Pelt-2::mCherry].Paper_evidenceWBPaper00038115
Curator_confirmedWBPerson712
EQ_annotationsLife_stageWBls:0000032PATO:0000460Paper_evidenceWBPaper00038115
Curator_confirmedWBPerson712
WBPhenotype:0001278Paper_evidenceWBPaper00038115
Curator_confirmedWBPerson712
RemarkLevels of daf-28 are reduced in wild type dauer larvae when compared to L2/L3 wild type animals, as assayed by the average relative intensity of fluorescence of ofEx345[Pdaf-28::GFP; Pelt-2::mCherry].Paper_evidenceWBPaper00038115
Curator_confirmedWBPerson712
EQ_annotationsLife_stageWBls:0000032PATO:0000460Paper_evidenceWBPaper00038115
Curator_confirmedWBPerson712
WBPhenotype:0001396Paper_evidenceWBPaper00038239
Curator_confirmedWBPerson712
RemarkIn wild-type worms, emodepside caused an almost complete inhibition of pumping.Paper_evidenceWBPaper00038239
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00004597Paper_evidenceWBPaper00038239
Curator_confirmedWBPerson712
WBPhenotype:0001470Paper_evidenceWBPaper00061941
Curator_confirmedWBPerson712
RemarkWe found that wild-type (dauer larvae) DL showed less odor attraction than corresponding adults for 2,3-butanedione, 2,3-pentanedione, and IAA, consistent with the results of a previous study that included 2,3-butanedione (Hallem et al. 2011)(Fig. 1A-C).Paper_evidenceWBPaper00061941
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00002819Paper_evidenceWBPaper00061941
Curator_confirmedWBPerson712
EQ_annotationsLife_stageWBls:0000032PATO:0000460Paper_evidenceWBPaper00061941
Curator_confirmedWBPerson712
WBPhenotype:0001472Paper_evidenceWBPaper00061941
Curator_confirmedWBPerson712
RemarkWe found that wild-type (dauer larvae) DL showed less odor attraction than corresponding adults for 2,3-butanedione, 2,3-pentanedione, and IAA, consistent with the results of a previous study that included 2,3-butanedione (Hallem et al. 2011)(Fig. 1A-C).Paper_evidenceWBPaper00061941
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00001063Paper_evidenceWBPaper00061941
Curator_confirmedWBPerson712
EQ_annotationsLife_stageWBls:0000032PATO:0000460Paper_evidenceWBPaper00061941
Curator_confirmedWBPerson712
WBPhenotype:0001500Paper_evidenceWBPaper00061941
Curator_confirmedWBPerson712
RemarkWe found that wild-type (dauer larvae) DL showed less odor attraction than corresponding adults for 2,3-butanedione, 2,3-pentanedione, and IAA, consistent with the results of a previous study that included 2,3-butanedione (Hallem et al. 2011)(Fig. 1A-C).Paper_evidenceWBPaper00061941
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00001715Paper_evidenceWBPaper00061941
Curator_confirmedWBPerson712
EQ_annotationsLife_stageWBls:0000032PATO:0000460Paper_evidenceWBPaper00061941
Curator_confirmedWBPerson712
WBPhenotype:0001588Paper_evidenceWBPaper00038206
Curator_confirmedWBPerson712
RemarkColchicine eliminated all microtubules in the soma, whereas other characteristics of the touch receptor neuron (TRN) physical attributes, such as the extracellular mantle, were maintained.Paper_evidenceWBPaper00038206
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00003637Paper_evidenceWBPaper00038206
Curator_confirmedWBPerson712
Phenotype_assayTreatmentAnimals were grown for multiple generations on plates containing 1 mM colchicine.Paper_evidenceWBPaper00038206
Curator_confirmedWBPerson712
GenotypeuIs43, uIs44(Pmec-18::praja::gfp)Paper_evidenceWBPaper00038206
Curator_confirmedWBPerson712
WBPhenotype:0001653Paper_evidenceWBPaper00005432
Curator_confirmedWBPerson712
RemarkWild-type animals tolerated cadmium exposure for extended periods of time.Paper_evidenceWBPaper00005432
Curator_confirmedWBPerson712
WBPhenotype:0001739Paper_evidenceWBPaper00046225
Curator_confirmedWBPerson712
RemarkDirect treatment of WT worms with the OEA analog improved physical activity maintenance in aged animals (Fig. 4H).Paper_evidenceWBPaper00046225
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00005442Paper_evidenceWBPaper00046225
Curator_confirmedWBPerson712
WBPhenotype:0001750Paper_evidenceWBPaper00060196
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00005358Paper_evidenceWBPaper00060196
Curator_confirmedWBPerson712
WBPhenotype:0001821Paper_evidenceWBPaper00003187
WBPaper00032342
Curator_confirmedWBPerson712
RemarkOnly ~15% of the animals accumulated at the border of the bacterial lawn.Paper_evidenceWBPaper00003187
Curator_confirmedWBPerson712
Animals foraged evenly throughout a lawn of bacteria as measured by a constant fluorescence ratio of GFP expressing bacteria at the border compared to center of a lawn.Paper_evidenceWBPaper00032342
Curator_confirmedWBPerson712
Phenotype_assayTreatmentAnimals were assayed on a bacterial lawn.Paper_evidenceWBPaper00003187
Curator_confirmedWBPerson712
Animals were grown on a lawn of E. coli constitutively expressing GFP, in which the fluorescent intensity in the outer 40% of the lawn (border) is 35% brighter than in the inner 60% (center), suggesting differences in bacterial density.Paper_evidenceWBPaper00032342
Curator_confirmedWBPerson712
WBPhenotype:0001871Paper_evidenceWBPaper00040160
Curator_confirmedWBPerson712
RemarkLifespan was extended by astragalan treatment.Paper_evidenceWBPaper00040160
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00005124Paper_evidenceWBPaper00040160
Curator_confirmedWBPerson712
WBPhenotype:0001893Paper_evidenceWBPaper00061762
Curator_confirmedWBPerson712
RemarkWe used the protonophore FCCP at various doses to decrease plasma membrane potential (m) in live C. elegans. m was monitored in live, adult worms using the potentiometric fluorescent indicator, TMRE. At all doses tested m was decreased compared to untreated control (Figure 1B).Paper_evidenceWBPaper00061762
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00007723Paper_evidenceWBPaper00061762
Curator_confirmedWBPerson712
Phenotype_assayStrainWBStrain00034066Paper_evidenceWBPaper00061762
Curator_confirmedWBPerson712
WBPhenotype:0002050Paper_evidenceWBPaper00040740
Curator_confirmedWBPerson712
RemarkExposure to 0.5 mg/ml of abamectin for 30 min resulted in paralysis of 98% of N2 animals.Paper_evidenceWBPaper00040740
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00003970Paper_evidenceWBPaper00040740
Curator_confirmedWBPerson712
WBPhenotype:0002079Paper_evidenceWBPaper00064301
Curator_confirmedWBPerson712
Remark"Animals on a high-glucose diet throughout their entire lifecycle have a delayed reproductive profile, with the median point of the fertile period (when 50% of the offspring have been produced) occurring later (Figs. 1C and 1E and Mondoux et al. 2011)." "In contrast to the effects of high-glucose diet on total offspring, which is a phenotype resulting from exposure during adulthood (Figs. 1A-B), we find that high-glucose diet during development is sufficient to delay the reproductive profile as much as exposure during the entire lifecycle." "High-glucose diet exposure only in development led to a 1-day delay in the reproductive profile (median at 3.6 days compared to 2.6 days for animals fed a control diet throughout the lifecycle; Figs. 1C and 1E), which was equal to the delay for animals exposed to glucose throughout their lifecycle (also median 3.6 days, Figs. 1C and 1E). High-glucose diet only in adulthood had a smaller but significant effect on reproductive timing (median 2.9 days; Figs. 1C and 1E), as these animals produced progeny more quickly than those exposed to glucose in development, but were still reproductively delayed compared to control diet. As we observed for total fertility, high-glucose diet only during L4 and the L4 to adult transition did not affect reproductive timing (Figs. 1D and 1F). These results suggest that both developmental and adult exposure to a high-glucose diet affect the reproductive profile, but that there is not an additive effect: high-glucose diet during adulthood does not further slow reproduction if the animal was exposed to high-glucose diet during development."Paper_evidenceWBPaper00064301
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00007746Paper_evidenceWBPaper00064301
Curator_confirmedWBPerson712
EQ_annotationsLife_stageWBls:0000041PATO:0000460Paper_evidenceWBPaper00064301
Curator_confirmedWBPerson712
WBPhenotype:0002109Paper_evidenceWBPaper00059998
WBPaper00062695
Curator_confirmedWBPerson712
Remark"...we examine the hatching rate of C. elegans wild-type (N2) embryos in response to acute (Triclosan) TCS exposure (see Methods). As shown in Fig. 1B-1D, TCS caused pronounced shrinkage of the inner cell mass of embryos in a dose-dependent fashion."Paper_evidenceWBPaper00059998
Curator_confirmedWBPerson712
DEC causes transient paralysis, and the worms start to recover 40 minutes post-treatment of 3mM DEC (Fig 1A). We plotted the concentration-response plot for the DEC-induced paralysis 30s post-treatment and found the IC50 to be 0.80.2mM (Fig 1B)Paper_evidenceWBPaper00062695
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00000656Paper_evidenceWBPaper00059998
Curator_confirmedWBPerson712
WBMol:00003038Paper_evidenceWBPaper00062695
Curator_confirmedWBPerson712
WBMol:00007968Paper_evidenceWBPaper00062695
Curator_confirmedWBPerson712
WBPhenotype:0002125Paper_evidenceWBPaper00040519
Curator_confirmedWBPerson712
RemarkExposure to selected coated silver nanoparticles (Ag NPs), PVP8, PVP38, CIT7, GA5 and GA22 resulted in an intermediate level of growth inhibition (30-50%). Toxcity rescue by chelator NAC and ROS-binding antioxidant, Trolox was comparable.Paper_evidenceWBPaper00040519
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00005288Paper_evidenceWBPaper00040519
Curator_confirmedWBPerson712
WBMol:00005293Paper_evidenceWBPaper00040519
Curator_confirmedWBPerson712
WBMol:00005294Paper_evidenceWBPaper00040519
Curator_confirmedWBPerson712
WBMol:00005290Paper_evidenceWBPaper00040519
Curator_confirmedWBPerson712
WBMol:00005295Paper_evidenceWBPaper00040519
Curator_confirmedWBPerson712
WBMol:00005296Paper_evidenceWBPaper00040519
Curator_confirmedWBPerson712
WBMol:00003315Paper_evidenceWBPaper00040519
Curator_confirmedWBPerson712
WBMol:00005289Paper_evidenceWBPaper00040519
Curator_confirmedWBPerson712
WBPhenotype:0002347Paper_evidenceWBPaper00065095
Curator_confirmedWBPerson712
RemarkWorms reared on plates wrapped in Parafilm displayed a lower amount of overall forward motion compared to worms reared on non-wrapped plates at baseline (Fig. 1G).Paper_evidenceWBPaper00065095
Curator_confirmedWBPerson712
In the Parafilm condition, the frequency of direction changes before taps was significantly lower than the non-Parafilm condition only for the first tap (Fig. 1J; Mann-Whitney U test, Tap 1 U = 278.5, p < 0.001; Tap 2 U = 188.0, p > 0.05; Tap 3 U = 192.0, p > 0.05; Tap 4 U = 193.0, p > 0.05), showing that at baseline, worms reared on Parafilm-wrapped plates make fewer spontaneous reversals than worms reared on non-wrapped plates. Similarly, the magnitude of direction changes after taps occur is only significantly different between conditions for the first tap (Fig. 1K; Mann-Whitney U test, Tap 1 U = 295.0, p < 0.001; Tap 2 U = 188.0, p > 0.05; Tap 3 U = 197.0, p > 0.05; Tap 4 U = 184.0, p > 0.05). As is the case for bias, frequency of direction change shifts to a comparable level in both conditions.Paper_evidenceWBPaper00065095
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00001774Paper_evidenceWBPaper00065095
Curator_confirmedWBPerson712
WBPhenotype:0002373Paper_evidenceWBPaper00048410
Curator_confirmedWBPerson712
RemarkC. elegans attraction increased for 24 h and remained high even after 72 h, similar to the trend observed in diacetyl production. C. elegans attraction to L. paracasei grown on citrate media correlates with diacetyl production, rather than bacterial growth patterns.Paper_evidenceWBPaper00048410
Curator_confirmedWBPerson712
WBPhenotype:0002430Paper_evidenceWBPaper00029334
Curator_confirmedWBPerson712
RemarkDifferent life stages of N2 or glp-1 nematodes exposed to 0, 100, or 200 J/m2 UVC exhibited marked differences in susceptibilityto induction of DNA damage (Figure 2), with starved L1 larvae the most and 1-day-old N2 adults the least susceptible.Paper_evidenceWBPaper00029334
Curator_confirmedWBPerson712
WBPhenotype:0002435Curator_confirmedWBPerson712
RemarkDifferent life stages of N2 or glp-1 nematodes exposed to 0,100, or 200 J/m2 UVC exhibited marked differences in susceptibility to induction of DNA damage (Figure 2), with starved L1 larvae the most and 1-day-old N2 adults the least susceptible.Curator_confirmedWBPerson712
EQ_annotationsLife_stageWBls:0000041PATO:0000460Curator_confirmedWBPerson712
WBPhenotype:0002436Curator_confirmedWBPerson712
RemarkDifferent life stages of N2 or glp-1 nematodes exposed to 0,100, or 200 J/m2 UVC exhibited marked differences in susceptibility to induction of DNA damage (Figure 2), with starved L1 larvae the most and 1-day-old N2 adults the least susceptible.Curator_confirmedWBPerson712
EQ_annotationsLife_stageWBls:0000024PATO:0000460Curator_confirmedWBPerson712
WBPhenotype:0002469Paper_evidenceWBPaper00065095
Curator_confirmedWBPerson712
Remark"Speed of response to the initial tap in the Parafilm condition was significantly lower than the initial response in the control condition (Fig. 1D; Mann-Whitney U test, Tap 1 U = 285.0, p < 0.001), but was significantly higher to the next 4 taps (Fig. 1D; Mann-Whitney U test, Tap 2 U = 81.0, p < 0.05; Tap 3 U = 8.0, p < 0.001; Tap 4 U = 40.0, p < 0.001; Tap 5 U = 52.0, p < 0.001), displaying a clear sensitization phenotype. Beginning at the 10th tap, there were no differences in reversal speed throughout the rest of the experiment and worms in both conditions habituated to similar speed levels after repeated stimulation (Fig. 1C)." Also "In the Parafilm condition, the frequency of direction changes before taps was significantly lower than the non-Parafilm condition only for the first tap (Fig. 1J; Mann-Whitney U test, Tap 1 U = 278.5, p < 0.001; Tap 2 U = 188.0, p > 0.05; Tap 3 U = 192.0, p > 0.05; Tap 4 U = 193.0, p > 0.05), showing that at baseline, worms reared on Parafilm-wrapped plates make fewer spontaneous reversals than worms reared on non-wrapped plates. Similarly, the magnitude of direction changes after taps occur is only significantly different between conditions for the first tap (Fig. 1K; Mann-Whitney U test, Tap 1 U = 295.0, p < 0.001; Tap 2 U = 188.0, p > 0.05; Tap 3 U = 197.0, p > 0.05; Tap 4 U = 184.0, p > 0.05). As is the case for bias, frequency of direction change shifts to a comparable level in both conditions."Paper_evidenceWBPaper00065095
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00001774Paper_evidenceWBPaper00065095
Curator_confirmedWBPerson712
WBPhenotype:0002545Paper_evidenceWBPaper00065910
Curator_confirmedWBPerson712
Remark...when compared to animals fed live OP50 and UVAb killed OP50, animals raised on PFA-killed E. coli OP50 were significantly smaller at the L4 larval stage (Figure 1A).";"...animals fed PFA-killed OP50 stored less lipids relative to animals fed live bacteria (Figure 1B-F).";"...Notably, the magnitude of this difference is more significant when compared to animals fed UV/antibiotic-killed bacteria, which display a modest increase in intracellular lipid pools.";"... Worms fed PFA-killed E. coli display a small but significant increase in the incidence of somatic depletion of fat, while maintaining germline lipid pools at day 4 of adulthood (Figure 1G-J)."; "...raising worms on PFA-treated OP50 led to a significant increase in average crawling speed. This increase in crawling speed was not observed in animals raised on the UV/Antibiotic-killed OP50."Paper_evidenceWBPaper00065910
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00008017Paper_evidenceWBPaper00065910
Curator_confirmedWBPerson712
WBMol:00004566Paper_evidenceWBPaper00065910
Curator_confirmedWBPerson712
WBPhenotype:0002650Paper_evidenceWBPaper00060230
Curator_confirmedWBPerson712
Remarklipopolysaccharide (LPS) extracted from S. enterica stimulates gravid adults to lay more eggs in LPS-containing solution than those in M9 buffer only. Figure 1 shows that the worms that were exposed to 0.1 mg/ml LPS for 1 hour laid an average of 5.570.57 eggs ( one standard error; n = 101), which was significantly different (p < 0.0001) than the response of the worms exposed to M9 buffer (1.870.25 eggs; n = 112).Paper_evidenceWBPaper00060230
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00007894Paper_evidenceWBPaper00060230
Curator_confirmedWBPerson712
WBPhenotype:0004022Paper_evidenceWBPaper00038239
Curator_confirmedWBPerson712
Remark100nM emodepside exposure (over 24 hours) elicited a flaccid paralysis, particularly apparent in the anterior of the worm, and a decrease in amplitude of the sinusoidal body shape. Emodepside inhibited body bends of wild-type worms after a shorter, 3 hour, incubation time but with a slightly higher IC50 (78 nM).Paper_evidenceWBPaper00038239
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00004597Paper_evidenceWBPaper00038239
Curator_confirmedWBPerson712
Phenotype_not_observed (22)
LocationCGC
Remark (30)
Reference (99)
SpeciesCaenorhabditis elegans
Wild_isolate
IsolationGPS51.46-2.6
PlaceBristol, Great Britain
LandscapeUrban_garden
Substratemushroom_compost
Sampled_byW. Nicholas
Isolated_byWBPerson2877
Date01 JAN 1951 00:00:00