WormBase Tree Display for RNAi: WBRNAi00114771
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| WBRNAi00114771 | Homol | Homol_homol | R06C7:RNAi | ||
|---|---|---|---|---|---|
| Sequence_info | PCR_product | sjj_R06C7.3 | |||
| Experiment | Strain | WBStrain00004126 | |||
| Genotype | unc-33(e204) | ||||
| Treatment | "For knockdown by feeding RNAi, bacterial cultures were plated on NGM plates supplemented with 25 μg/mL carbenicillin, 10 μg/mL tetracycline, and 1 mM IPTG, and incubated for 3 days at room temperature to induce double-stranded RNA. L4 worms at 20°C were fed bacteria for 48 hours and then singled to individual plates. After laying eggs for approximately 18 hours, the adults were removed and all progeny were counted via a dissecting microscope. After an additional 24 hours, the remaining number of eggs were counted." | ||||
| Temperature | 20 | ||||
| Delivered_by | Bacterial_feeding | ||||
| Inhibits | Predicted_gene | R06C7.3 | Inferred_automatically | RNAi_primary | |
| Gene | WBGene00000963 | Inferred_automatically | RNAi_primary | ||
| Transcript | R06C7.3.1 | Inferred_automatically | RNAi_primary | ||
| R06C7.3.2 | Inferred_automatically | RNAi_primary | |||
| Species | Caenorhabditis elegans | ||||
| Reference | WBPaper00060038 | ||||
| Phenotype_not_observed | WBPhenotype:0000351 | Remark | "In both wildtype and unc-33(e204) homozygous animals, knockdown of dhp-1 did not significantly alter the percent of embryos that failed to hatch (3.1% and 19.5%, respectively)." | ||
| Remark | dhp-1 (WBGene00000963) RNAi (Ahringer library clone) | ||||
| Method | RNAi |
