WormBase Tree Display for RNAi: WBRNAi00101625
expand all nodes | collapse all nodes | view schema
WBRNAi00101625 | Homol | Homol_homol | K04G2:RNAi | ||
---|---|---|---|---|---|
Sequence_info | PCR_product | sjj_K04G2.8 | |||
Experiment | Laboratory | CT | |||
Date | 01 Nov 2010 00:00:00 | ||||
Genotype | wIs78 [SCMp::GFP marker transgene for seam cells]; lin-42(n1089) | ||||
Treatment | RNAi was induced by feeding, starting with L1 stage starved and synchronized animals. RNAi constructs were expressed from E. coli HT115(DE3) bacteria, which were grown on NGM media containing 1 mM IPTG to induce expression from the convergent T7 polymerase promoters on the pL4440 vector. | ||||
Temperature | 20 | ||||
Delivered_by | Bacterial_feeding | ||||
Inhibits | Predicted_gene | K04G2.8c | Inferred_automatically | RNAi_primary | |
K04G2.8b | Inferred_automatically | RNAi_primary | |||
K04G2.8a | Inferred_automatically | RNAi_primary | |||
Gene | WBGene00000156 | Inferred_automatically | RNAi_primary | ||
Transcript | K04G2.8c.1 | Inferred_automatically | RNAi_primary | ||
K04G2.8b.1 | Inferred_automatically | RNAi_primary | |||
K04G2.8a.1 | Inferred_automatically | RNAi_primary | |||
Species | Caenorhabditis elegans | ||||
Reference | WBPaper00037838 | ||||
Phenotype_not_observed | WBPhenotype:0000168 | Remark | RNAi did not significantly affect the precocious production of alae in L4 larvae animals carrying the lin-42(n1089) mutation | ||
Remark | (Figure 7C) apr-1 RNAi | ||||
Method | RNAi |