WormBase Tree Display for RNAi: WBRNAi00101600
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| WBRNAi00101600 | Homol | Homol_homol | K04G2:RNAi | ||
|---|---|---|---|---|---|
| Sequence_info | PCR_product | sjj_K04G2.8 | |||
| Experiment | Laboratory | CT | |||
| Date | 01 Nov 2010 00:00:00 | ||||
| Genotype | wIs78 [SCMp::GFP marker transgene for seam cells] | ||||
| Treatment | RNAi was induced by feeding, starting with L1 stage starved and synchronized animals. RNAi constructs were expressed from E. coli HT115(DE3) bacteria, which were grown on NGM media containing 1 mM IPTG to induce expression from the convergent T7 polymerase promoters on the pL4440 vector. All strains feeding on RNAi bacteria were cultured at 20 degrees Celsius unless otherwise noted. | ||||
| Temperature | 20 | ||||
| Delivered_by | Bacterial_feeding | ||||
| Inhibits | Predicted_gene | K04G2.8c | Inferred_automatically | RNAi_primary | |
| K04G2.8b | Inferred_automatically | RNAi_primary | |||
| K04G2.8a | Inferred_automatically | RNAi_primary | |||
| Gene | WBGene00000156 | Inferred_automatically | RNAi_primary | ||
| Transcript | K04G2.8c.1 | Inferred_automatically | RNAi_primary | ||
| K04G2.8b.1 | Inferred_automatically | RNAi_primary | |||
| K04G2.8a.1 | Inferred_automatically | RNAi_primary | |||
| Species | Caenorhabditis elegans | ||||
| Reference | WBPaper00037838 | ||||
| Phenotype_not_observed | WBPhenotype:0000168 | Remark | RNAi did not affect the formation of alae in young adult animals | ||
| Remark | (Figure 7A) apr-1 RNAi | ||||
| Method | RNAi |
