WormBase Tree Display for RNAi: WBRNAi00101535

WBRNAi00101535 Homol: Homol_homol: K04G2:RNAi
  Sequence_info: PCR_product: sjj_K04G2.8
  Experiment: Laboratory: CT
    Date: 01 Nov 2010 00:00:00
    Genotype: wIs78 [SCMp::GFP marker transgene for seam cells]; lit-1(RNAi)
    Treatment: RNAi was induced by feeding, starting with L1 stage starved and synchronized animals. RNAi constructs were expressed from E. coli HT115(DE3) bacteria, which were grown on NGM media containing 1 mM IPTG to induce expression from the convergent T7 polymerase promoters on the pL4440 vector. All strains feeding on RNAi bacteria were cultured at 20 degrees Celsius unless otherwise noted. Simultaneous RNAi was carried out by growing the two different RNAi bacterial strains to the same optical density and mixing equal volumes of the cultures before plating onto NGM plates.
    Temperature: 20
    Delivered_by: Bacterial_feeding
  Inhibits: Predicted_gene: K04G2.8c Inferred_automatically: RNAi_primary
      K04G2.8b Inferred_automatically: RNAi_primary
      K04G2.8a Inferred_automatically: RNAi_primary
    Gene: WBGene00000156 Inferred_automatically: RNAi_primary
    Transcript: K04G2.8c.1 Inferred_automatically: RNAi_primary
      K04G2.8b.1 Inferred_automatically: RNAi_primary
      K04G2.8a.1 Inferred_automatically: RNAi_primary
  Species: Caenorhabditis elegans
  Reference: WBPaper00037838
  Phenotype_not_observed: WBPhenotype:0001887 Remark: apr-1(RNAi) did not have any effect on the fewer seam cells phenotype of lit-1(RNAi), suggesting that lit-1(RNAi) is epistatic to apr-1(RNAi)
    WBPhenotype:0001923 Remark: apr-1(RNAi) did not have any effect on the fewer seam cells phenotype of lit-1(RNAi), suggesting that lit-1(RNAi) is epistatic to apr-1(RNAi)
  Remark: (Table 1) apr-1 RNAi (double RNAi with lit-1; see genotype)
  Method: RNAi