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WormBase Tree Display for RNAi: WBRNAi00101453

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Name Class

WBRNAi00101453HomolHomol_homolB0336:RNAi
Sequence_infoDNA_textATGGATGTGGATTGCGCAGAAACATTCTCGCAACCATGCACTCCGCTGAATTTCAATCCGATGACTCCGTCAACATCGAGAGTTTCAACACCAGTTCGGCCGTCTTCTACAATGTCTGCCAGACAATATTCAGGTTCGCCGTTCAAGGCTCAACCTCAGAACATGGAACCGTCCAATTCTCGGGTGCAAGAACTCCGGGAAGCTGCTGTGGGAAAGCGATCATACACAAATGCTTGGATGCAGGGTACCTATGCACCGCCGCAGATGGCAGGACAACAACAAAGGTTTTCGAGGCCTCCGAGTGTGATCGGCTCCACAATGTCTCACATGACGAATATGTCAGAAATGACCGCCTACTCGTACGGTGGATTGTCAATGCTCTCAGTCAACACTGAAATGGGTGAATTCAACAATTTTGTCAATCAGGCACCATATCAGCGAGCACTGACACGTGTATCGCAGGTTTCCGAGAATCAGGATCCGACGAATCGACAGTATCCGATGACAGCTCCCGAGATTATTGAGAATCTGGAATCAACGGAACTAATCAATCAAGCAGCAGCGATTCGAGCTTTGGAGCCAATTGTGAAAGCTGGTGGAATGTTGCAAACCTGGGGACCTAAAGGAGCCGAACCGATTATTCGAGCTCT
ExperimentLaboratoryJK
Date23 Oct 2001 00:00:00
TreatmentdsRNA to each gene was injected into N2 at both 2.5 and 1 mg/ml. Injected animals were crossed with N2 males or males carrying GFP reporters.
Delivered_byInjection
InhibitsPredicted_geneB0336.1aInferred_automaticallyRNAi_primary
B0336.1bInferred_automaticallyRNAi_primary
GeneWBGene00006943Inferred_automaticallyRNAi_primary
TranscriptB0336.1b.1Inferred_automaticallyRNAi_primary
B0336.1a.1Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
ReferenceWBPaper00005116
PhenotypeWBPhenotype:0001586Remarkwrm-1 RNAi resulted in a completely penetrant Sys (somatic gonad precursor symmetrical sisters) phenotype as indicated by missing distal tip cells (in males and hermaphrodites), extra anchor cells (in hermaphrodites), and extra linker cells (in males)
PenetranceComplete
EQ_annotationsAnatomy_termWBbt:0006865PATO:0000460
WBPhenotype:0001962Remarkwrm-1 RNAi resulted in a completely penetrant Sys (somatic gonad precursor symmetrical sisters) phenotype as indicated by missing distal tip cells (in males and hermaphrodites), extra anchor cells (in hermaphrodites), and extra linker cells (in males)
PenetranceComplete
EQ_annotationsAnatomy_termWBbt:0006865PATO:0000460
WBPhenotype:0001968Remarkwrm-1 RNAi resulted in a completely penetrant Sys (somatic gonad precursor symmetrical sisters) phenotype as indicated by missing distal tip cells (in males and hermaphrodites), extra anchor cells (in hermaphrodites), and extra linker cells (in males)
PenetranceComplete
EQ_annotationsAnatomy_termWBbt:0006865PATO:0000460
WBPhenotype:0002189Remarkwrm-1 RNAi resulted in a completely penetrant Sys (somatic gonad precursor symmetrical sisters) phenotype as indicated by missing distal tip cells (in males and hermaphrodites), extra anchor cells (in hermaphrodites), and extra linker cells (in males)
PenetranceComplete
EQ_annotationsAnatomy_termWBbt:0006865PATO:0000460
Remark(Table 4) wrm-1 RNAi. Authors report generating dsRNA by RT-PCR to the 5' end of the gene and that clones contained at least 650bp of exon sequence. First 650bp of coding region of cDNA used for curation.
MethodRNAi