WormBase Tree Display for RNAi: WBRNAi00097538
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| WBRNAi00097538 | Homol | Homol_homol | F11A6:RNAi | ||
|---|---|---|---|---|---|
| Sequence_info | PCR_product | sjj_F11A6.2 | |||
| Experiment | Laboratory | WS | |||
| Date | 02 May 2007 00:00:00 | ||||
| Genotype | ced-5(n1812); opIs117[Phsp::sel-1sp::GFP::AnxV] (opIs117 is a marker for phospholipid bilayer outer leaflet phosphatidylserine (PS) exposure, an indicator of apoptosis) | ||||
| Treatment | Approximately 30-50 synchronized ced-5(n1812); opIs117 L1 larvae were transferred onto 2 mM IPTG RNAi plates seeded with bacteria expressing RNAi constructs. Worms were grown for 3 days, then heat shocked at 33 degrees Celsius for 30 min. Six hours after heat shock (24 hr after the L4/adult molt stage), germ-cell corpses were counted with Nomarski optics (DIC) and GFP epifluorescence. | ||||
| Delivered_by | Bacterial_feeding | ||||
| Inhibits | Predicted_gene | F11A6.2 | Inferred_automatically | RNAi_primary | |
| Gene | WBGene00008681 | Inferred_automatically | RNAi_primary | ||
| Transcript | F11A6.2.1 | Inferred_automatically | RNAi_primary | ||
| Species | Caenorhabditis elegans | ||||
| Reference | WBPaper00030738 | ||||
| Phenotype | WBPhenotype:0000241 | Remark | RNAi results in an increase in the number of apoptotic germ cell corpses, as determined by Nomarski/DIC optics (for germ cell corpses) and GFP epifluorescence (for phosphatidylserine exposure labeled by GFP::AnxV) | ||
| Remark | (Figure S2A) scrm-4/F11A6.2 RNAi | ||||
| Method | RNAi |
