WormBase Tree Display for RNAi: WBRNAi00092576
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| WBRNAi00092576 | Homol | Homol_homol | T26E3:RNAi | ||
|---|---|---|---|---|---|
| Sequence_info | DNA_text | ATGCCACTTGGAAAGTTGGACCTAGTCGAAGAGGAGTACATCGCCGAAAGCGGAGATCACACGCCAGAAGCCTCCCGGCTTATGGGAAATGGTGATTCTGTAGTTGAAAACTTGAATGGTCACACAAATGGGGCAGTTGCGAAGAAGAAAAATGAGCCAAGGGTGCCGGATATGCAGTTGGGCAAATGTCGCTATGTAAGGTTACATGACAATGTAAACTATGTCGCTGCTGCAATTATTCTACGGAACCAGGGAGACGACACAGAGGTGCTCCTAATCCAAGAGGCCAAGAAAAGTTGTCGTGGGAAATGGTATATGCCTGCCGGACGGGTAGAAGCTGGTGAGACAATTGAAGAAGCAGTGGTACGAGAGGTTAAAGAGGAGACTGGGTACAGTTGTGATGTTGTGGAACTCCTGTCACTACAAGTTCAAGGATCCGGATGGTATCGATATGCTTTTTATTGTAATATTACAGGCGGTGACCTGAAAACAGAACCCGATCAGGAATCCCTAGCCGCAGAGTGGTATAACATCAAGGATCTCAAAGCCAACAAGGTTCAGTTAAGAGGCCGAGATTTCATCCGACTAGTCGACGAGGCCGTCACATACCGAACACACGGCCCCGTCGATTCTATTCCACGTGTAATGCCGTTAAATCAAAACGTTGCTGGATTATTTTTGGAGTTTATGATTGTCAAGCACTCTAGGGATGGGCTCCGAACCGAGGTGCTCGTGCACAAGTCGATCAAGGACGAGACGTACCTGCTGGAAGAGGAACAGCCATTCCCAACGGTGGAATTTGGGTTCGAGTACTTCTTTGCGATGGTTGTATCCAAGTGTTATAGGCATTTGCTCGAAGAAGGTGCCAATGTAGTATTTACACCGTCTCACGTGACGCGGATAAAATGCCACCCAAAACCAATGGAATCCCTGGCGCACGGAGTCTCAATAAGGGTTTACTGCGAGCACAAGCAATCCGCTTCAAAAGCCATCATTCGGAGTCCGCGGTATCACTGGATATCCGTCGAATCACCGGAGACACGACAGCGATTTCACATGGCTCAGAAGCAATTCCGGCCGTCACTTCATATGCTTTAA | |||
| Experiment | Date | 28 Jul 2011 00:00:00 | |||
| Strain | WBStrain00000001 | ||||
| Treatment | Worms were harvested by rinsing with S buffer (100mM NaCl, 50mM potassium phosphate, pH 6.0) and resuspended in alkaline hypochlorite (500mM NaOH and 1.2% hypochlorite) until the adults dissolved (510 min). Eggs were then washed five times with S buffer. The eggs were transferred to NGM plates or RNAi-plates, allowed to develop to adults (about 3 days) and moved to fresh NGM plates or RNAi plates (810 worms/plate) supplemented with 40M 5-fluoro-20-deoxyuridine to suppress the production of progeny. The day when young adults were moved was taken as day 0, and the living populations were counted every 2 days. RNAi worms were moved to new RNAi plates every 6 days. Whether a nematode was alive or dead was judged by its response to tapping it on the plate. The experiments were performed at 20 degrees Celsius. | ||||
| Temperature | 20 | ||||
| Delivered_by | Bacterial_feeding | ||||
| Inhibits | Predicted_gene | T26E3.2 | Inferred_automatically | RNAi_primary | |
| Gene | WBGene00003578 | Inferred_automatically | RNAi_primary | ||
| Transcript | T26E3.2.1 | Inferred_automatically | RNAi_primary | ||
| Species | Caenorhabditis elegans | ||||
| Reference | WBPaper00040133 | ||||
| Phenotype_not_observed | WBPhenotype:0000039 | Remark | ndx-1 RNAi did not significantly affect the life span of wild type (N2) worms | ||
| Remark | (Figure 4B) ndx-1 RNAi | ||||
| Method | RNAi |
