WormBase Tree Display for RNAi: WBRNAi00092474
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| WBRNAi00092474 | Homol | Homol_homol | K07C11:RNAi | ||
|---|---|---|---|---|---|
| Sequence_info | DNA_text | ATGAGCGGAAAGGAAAATACTGCTCCTGTGATAGACGATCAGAAGGCCGAGGTTATTTCACTTACGGAAGATAGCAGACCTCAGAGAGTTGACCAGGCAAGGGAAGAATCATGCTGGAGTCTTGATGATTTTGACGTTGGACGGCCTCTCGGAAAAGGAAAGTTTGGAAACGTTTTCATCTCTCGTGAAAAAAAGACCAAGCGAATCATAGCTTTGAAAGTACTCTTCAAAACACAGTTGCTTCAACTTGGAGTCAGTCATCAGTTGAAGAGAGAGATCGAAATTCAATATCATTTGCGTCATCCGAATATTCTCACTCTTTATGGATACTTTCACGATGACAAGCGTGTGTTCGTCATTCTCGACTATGCCAGCAGAGGAGAATTATTTAATGTCCTCCAATCACAACCTGGACACAAAGTGAATGAGGTCATTGCCGGAAGATTTGTGCGACAGCTGGCCAATGCGTTGCATTACTGCCATTCGAAAGGAGTTATTCACAGAGACATTAAGCCTGAAAATCTTCTTCTTGATTCGAAGCTCAATCTGAAGCTTGCCGATTTCGGATGGTCCGTCGTTGCTGATCACTCGAAACGCCATACATTGTGCGGTACCATGGATTATCTTGCTCCGGAAATGGTTTCCAATCAGCCACATGACTTTAATGTTGACATTTGGGCAATTGGAATTCTTCTCTTCGAAATGCTTGTTGGATATGCTCCGTTCGCCAATCAAACTGGTGATAAATTGATCGCCAGAATCAAGGAGTGCAAGATTTATATTCCAAGTGTTGTGACTGACGGAGCTGCCAGTTTGATAAATGCAATAATCAAAAAGGAACCCCAAGAACGTCTCCCACTTGTTGACATCATGGCTCATCCGTGGATTAAGGAAATGAAGCAACGCGAAGACATTGAAGTTCCACTCTTCATTTCGACGCTCACGAAATCCAGTCGCAATAATTCTACAGCCAATCAATAA | |||
| Experiment | Laboratory | AG | |||
| Date | 24 Aug 1998 00:00:00 | ||||
| Treatment | L4 and adult N2 hermaphrodites were injected with antisense RNA by standard methods and were allowed to recover for 12-24 hours before fixation for immunocytochemistry. | ||||
| Delivered_by | Injection | ||||
| Inhibits | Predicted_gene | K07C11.2 | Inferred_automatically | RNAi_primary | |
| Gene | WBGene00000098 | Inferred_automatically | RNAi_primary | ||
| Transcript | K07C11.2.1 | Inferred_automatically | RNAi_primary | ||
| Species | Caenorhabditis elegans | ||||
| Reference | WBPaper00003325 | ||||
| Phenotype | WBPhenotype:0001403 | Remark | In air-1(RNAi) embryos, P-granules and PIE-1 were localized to a single blastomere in approximately half of the examined embryos. In some cases, the staining blastomere was completely anucleate. Although PIE-1 was readily apparent on mitotic centrosomes in wild-type embryos, it was never detected on centrosomes in air-1(RNAi) embryos (n > 200embryos). Defects in P-granule and PIE-1 localization that were observed in 1-cell and 2-cell air-1(RNAi) embryos included the bipolar localization of both P-granules and PIE-1, or bipolar P-granules with PIE-1 localized to only one pole. In older embryos, P-granules were often mislocalized to more than one blastomere and, in the majority of these cases, the PIE-1 protein was undetectable. P-granules were often clumped around many nuclei in older embryos while PIE-1 staining was absent. | ||
| Remark | (Figure 7, Table 3) air-1 RNAi | ||||
| Method | RNAi |
