WormBase Tree Display for RNAi: WBRNAi00092472

WBRNAi00092472 Homol: Homol_homol: K07C11:RNAi
  Sequence_info: DNA_text: ATGAGCGGAAAGGAAAATACTGCTCCTGTGATAGACGATCAGAAGGCCGAGGTTATTTCACTTACGGAAGATAGCAGACCTCAGAGAGTTGACCAGGCAAGGGAAGAATCATGCTGGAGTCTTGATGATTTTGACGTTGGACGGCCTCTCGGAAAAGGAAAGTTTGGAAACGTTTTCATCTCTCGTGAAAAAAAGACCAAGCGAATCATAGCTTTGAAAGTACTCTTCAAAACACAGTTGCTTCAACTTGGAGTCAGTCATCAGTTGAAGAGAGAGATCGAAATTCAATATCATTTGCGTCATCCGAATATTCTCACTCTTTATGGATACTTTCACGATGACAAGCGTGTGTTCGTCATTCTCGACTATGCCAGCAGAGGAGAATTATTTAATGTCCTCCAATCACAACCTGGACACAAAGTGAATGAGGTCATTGCCGGAAGATTTGTGCGACAGCTGGCCAATGCGTTGCATTACTGCCATTCGAAAGGAGTTATTCACAGAGACATTAAGCCTGAAAATCTTCTTCTTGATTCGAAGCTCAATCTGAAGCTTGCCGATTTCGGATGGTCCGTCGTTGCTGATCACTCGAAACGCCATACATTGTGCGGTACCATGGATTATCTTGCTCCGGAAATGGTTTCCAATCAGCCACATGACTTTAATGTTGACATTTGGGCAATTGGAATTCTTCTCTTCGAAATGCTTGTTGGATATGCTCCGTTCGCCAATCAAACTGGTGATAAATTGATCGCCAGAATCAAGGAGTGCAAGATTTATATTCCAAGTGTTGTGACTGACGGAGCTGCCAGTTTGATAAATGCAATAATCAAAAAGGAACCCCAAGAACGTCTCCCACTTGTTGACATCATGGCTCATCCGTGGATTAAGGAAATGAAGCAACGCGAAGACATTGAAGTTCCACTCTTCATTTCGACGCTCACGAAATCCAGTCGCAATAATTCTACAGCCAATCAATAA
  Experiment: Laboratory: AG
    Date: 24 Aug 1998 00:00:00
    Treatment: L4 and adult N2 hermaphrodites were injected with antisense RNA by standard methods and were allowed to recover for 12-24 hours before fixation for immunocytochemistry.
    Delivered_by: Injection
  Inhibits: Predicted_gene: K07C11.2 Inferred_automatically: RNAi_primary
    Gene: WBGene00000098 Inferred_automatically: RNAi_primary
    Transcript: K07C11.2.1 Inferred_automatically: RNAi_primary
  Species: Caenorhabditis elegans
  Reference: WBPaper00003325
  Phenotype: WBPhenotype:0000628 Remark: 22% of AB cells and 22% of P1 cells in the air-1 RNAi 2-cell embryo exhibited small spindles. Spindles were designated as "small" if they had smaller centrosomes and/or shorter microtubules (microtubules do not reach the cell cortex) as compared to wild-type spindles at similar stages of the cell cycle
      EQ_annotations: Life_stage: WBls:0000007 PATO:0000460
    WBPhenotype:0000759 Remark: 28% of AB cells and 31% of P1 cells in the air-1 RNAi 2-cell embryo exhibited an inappropriate interphase-like array of spindles during mitosis. An interphase-like array was judged as inappropriate if it was found in a cell with condensed chromatin
      EQ_annotations: Life_stage: WBls:0000007 PATO:0000460
    WBPhenotype:0000765 Remark: 3% of AB cells and 6% of P1 cells in the air-1 RNAi 2-cell embryo exhibited a stretched midbody. Anaphase spindles that were longer than wild type appeared to have "stretched midbodies" as compared to wild-type controls
      EQ_annotations: Life_stage: WBls:0000007 PATO:0000460
    WBPhenotype:0001106 Remark: 6% of air-1 RNAi 2-cell embryos exhibited both spindles in the same orientation
      EQ_annotations: Life_stage: WBls:0000007 PATO:0000460
    WBPhenotype:0001588 Remark: 22% of AB cells and 22% of P1 cells in the air-1 RNAi 2-cell embryo exhibited spindles with fewer microtubules. Spindles were designated as having fewer microtubules if the microtubule density (as assessed by eye) was much less than a wild-type spindle
      EQ_annotations: Life_stage: WBls:0000007 PATO:0000460
    WBPhenotype:0001903 Remark: 3% of AB cells in the air-1 RNAi 2-cell embryo exhibited multiple centrosomes (more than two per cell).
      EQ_annotations: Life_stage: WBls:0000007 PATO:0000460
  Remark: (Table 2) air-1 RNAi
  Method: RNAi