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WormBase Tree Display for RNAi: WBRNAi00092468

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Name Class

WBRNAi00092468HomolHomol_homolK07C11:RNAi
Sequence_infoDNA_textATGAGCGGAAAGGAAAATACTGCTCCTGTGATAGACGATCAGAAGGCCGAGGTTATTTCACTTACGGAAGATAGCAGACCTCAGAGAGTTGACCAGGCAAGGGAAGAATCATGCTGGAGTCTTGATGATTTTGACGTTGGACGGCCTCTCGGAAAAGGAAAGTTTGGAAACGTTTTCATCTCTCGTGAAAAAAAGACCAAGCGAATCATAGCTTTGAAAGTACTCTTCAAAACACAGTTGCTTCAACTTGGAGTCAGTCATCAGTTGAAGAGAGAGATCGAAATTCAATATCATTTGCGTCATCCGAATATTCTCACTCTTTATGGATACTTTCACGATGACAAGCGTGTGTTCGTCATTCTCGACTATGCCAGCAGAGGAGAATTATTTAATGTCCTCCAATCACAACCTGGACACAAAGTGAATGAGGTCATTGCCGGAAGATTTGTGCGACAGCTGGCCAATGCGTTGCATTACTGCCATTCGAAAGGAGTTATTCACAGAGACATTAAGCCTGAAAATCTTCTTCTTGATTCGAAGCTCAATCTGAAGCTTGCCGATTTCGGATGGTCCGTCGTTGCTGATCACTCGAAACGCCATACATTGTGCGGTACCATGGATTATCTTGCTCCGGAAATGGTTTCCAATCAGCCACATGACTTTAATGTTGACATTTGGGCAATTGGAATTCTTCTCTTCGAAATGCTTGTTGGATATGCTCCGTTCGCCAATCAAACTGGTGATAAATTGATCGCCAGAATCAAGGAGTGCAAGATTTATATTCCAAGTGTTGTGACTGACGGAGCTGCCAGTTTGATAAATGCAATAATCAAAAAGGAACCCCAAGAACGTCTCCCACTTGTTGACATCATGGCTCATCCGTGGATTAAGGAAATGAAGCAACGCGAAGACATTGAAGTTCCACTCTTCATTTCGACGCTCACGAAATCCAGTCGCAATAATTCTACAGCCAATCAATAA
ExperimentLaboratoryAG
Date24 Aug 1998 00:00:00
TreatmentL4 and adult N2 hermaphrodites were injected with antisense RNA by standard methods and were allowed to recover for 12-24 hours before fixation for immunocytochemistry.
Delivered_byInjection
InhibitsPredicted_geneK07C11.2Inferred_automaticallyRNAi_primary
GeneWBGene00000098Inferred_automaticallyRNAi_primary
TranscriptK07C11.2.1Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
ReferenceWBPaper00003325
PhenotypeWBPhenotype:0000773Remark20% of air-1 RNAi embryos at the one-cell stage exhibited free chromosomes. A cell was designated as having a free chromosome or chromosomes if at least one chromosome was found to be well separated from the main chromatinmass of the cell.
PenetranceRange20
EQ_annotationsLife_stageWBls:0000006PATO:0000460
WBPhenotype:0001027Remark8% of air-1 RNAi embryos at the one-cell stage exhibited misplaced nuclei
PenetranceRange8
EQ_annotationsLife_stageWBls:0000006PATO:0000460
WBPhenotype:0001348Remark5% of air-1 RNAi embryos at the one-cell stage exhibited hyper-condensed chromatin
PenetranceRange5
EQ_annotationsLife_stageWBls:0000006PATO:0000460
WBPhenotype:0001362Remark34% of air-1 RNAi embryos at the one-cell stage exhibited string-like (unraveling, fragmented or decondensed) chromatin
PenetranceRange34
EQ_annotationsLife_stageWBls:0000006PATO:0000460
WBPhenotype:0001584Remark14% of air-1 RNAi embryos at the one-cell stage exhibited wrong orientation. Wrong orientation refers to metaphase or anaphase cells that had chromosomes that were aligned in an abnormal orientation as compared to wild-type cells.For instance, some abnormal 1-cell mitotic embryos had anaphase figures that were perpendicular to the long axis of the embryo rather than parallel to the longaxis as in wild-type cells of the same stage.
PenetranceRange14
EQ_annotationsLife_stageWBls:0000006PATO:0000460
WBPhenotype:0001875Remark31% of air-1 RNAi embryos at the one-cell stage exhibited chromatin bridges
PenetranceRange31
EQ_annotationsLife_stageWBls:0000006PATO:0000460
WBPhenotype:0001882Remark17% of air-1 RNAi embryos at the one-cell stage exhibited polyploidy
PenetranceRange17
EQ_annotationsLife_stageWBls:0000006PATO:0000460
WBPhenotype:0001895Remark19% of air-1 RNAi embryos at the one-cell stage exhibited failed pronuclear fusion
PenetranceRange19
EQ_annotationsLife_stageWBls:0000006PATO:0000460
Remark(Table 1) air-1 RNAi
MethodRNAi