WormBase Tree Display for RNAi: WBRNAi00092452
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| WBRNAi00092452 | Homol | Homol_homol | CHROMOSOME_III:RNAi | ||
|---|---|---|---|---|---|
| Sequence_info | DNA_text | ATGGATGTGGATTGCGCAGAAACATTCTCGCAACCATGCACTCCGCTGAATTTCAATCCGATGACTCCGTCAACATCGAGAGTTTCAACACCAGTTCGGCCGTCTTCTACAATGTCTGCCAGACAATATTCAGGTTCGCCGTTCAAGGCTCAACCTCAGAACATGGAACCGTCCAATTCTCGGGTGCAAGAACTCCGGGAAGCTGCTGTGGGAAAGCGATCATACACAAATGCTTGGATGCAGGGTACCTATGCACCGCCGCAGATGGCAGGACAACAACAAAGGTTTTCGAGGCCTCCGAGTGTGATCGGCTCCACAATGTCTCACATGACGAATATGTCAGAAATGACCGCCTACTCGTACGGTGGATTGTCAATGCTCTCAGTCAACACTGAAATGGGTGAATTCAACAATTTTGTCAATCAGGCACCATATCAGCGAGCACTGACACGTGTATCGCAGGTTTCCGAGAATCAGGATCCGACGAATCGACAGTATCCGATGACAGCTCCCGAGATTATTGAGAATCTGGAATCAACGGAACTAATCAATCAAGCAGCAGCGATTCGAGCTTTGGAGCCAATTGTGAAAGCTGGTGGAATGTTGCAAACCTGGGGACCTAAAGGAGCCGAACCGATTATTCGAGCTCTGTTTCAAGTTCTTATTCCTCGGCCAGTTGAGAATGAGAATGTAATCCGGAAAGCGTTTGAAATTCTCCACCACTCAATTCTTCTCTCCAACAAGGAAATCCGACGTATCGACAGGATGTTCTTCCGACTGAATGCGGCATTAATGGATCCCAATGGGCCGCCTGTGTTCAATGTACCAAAGCCATACAGTATCTATGAGATCGTGATGACACGTGCCATTCAACTTGACACGAAATTCGAGTCCAGTGCTATGGTTCTGCTCGTACATCTTTGTTGTAAACCTCATTTCATGAAGATTTTCTTTGGAGAAGATGAGACTCAACAGTCACCAGCACATCGACGTCTTCACAAAATAGTAATTGAATTTGCAATTGGAAACCTGAGAAGACCGGAAACGAAGTCAAAGAATAAGGGACTTTGTGTATCGATTATTAAGAATTTGTCGAATAAAAATGCAACTATCAAAGATATGTCAGAACGTCTTGGTGTTGTGAGCCTCTTTCATCAAATTATGCAGAACGAAGTGATCCACGAGGATTTGTTGTGGTCAACGATGCAGGCTCTTACCGTATTCTGTGGAGATGTTAAGAATGGAACACATTTTGTGCAAATGGGTGGAGCACAAGTTTTGTGCGGTCTTCTTTCGCATGGATCAACACGACTTCTTCACGAACTTCTGAAGTGTTTGAGACGAGTATCAGATTTGCCAGCGATCCAGGAGCAGGATATGAAAGAATCGATTCATTGTATAGTTCAACTTATTGGATGTTCGGATGTAACGATTGTTGAGCTAGCGACTGGAACTCTACGAAATATTGGACTTCATAACAAGATGAACAAGGCATTTATGGTGCAGGATGGAGTTACAAGTCATGCGATCGCAGTTCTTCGGACATCTGAACAGTTTACATATCAGCCACATGCCAATATAGATTTATATAGAAAGCAGATTCTGTCGATTTATGAAAATTGTCTATCCGTATTGAACAATGTGACTTCTATGGCACCTCAAGATATCAAAGAATCTGCAGTATCAGCGTGTAGAATGATCAGTGAGAATGCCGACTCCGCATATGTGCTCCTTCATTACTTTAACGTTGGAAATCGGAAGTGCAGGAAACTTGCGGTGACAGTAATGAAACGTGTCATTGAGACGGTTCCTGCTTTTGCTGATCCGTTTGTTGATCTACTTGGAACAACAAATGAGCCACTACCAATTTTATTGCTTCAAAGAGCATTCCAGAGTTTGGACGAATGGCGGAAAACTAGCGTTGAAATGATGAATTGTGACGGTAGAAGTGCTGAGCAGAGAAGAGAATTAGATGACAGACGAAAAGATCACGAAGATATTGTAAAACGATCTGTGGGACTTTTAACGAATCTTTGTAGTCAGGCAAATCCAAGATTTTTCCACAGTTTGAAGCTAGTATTGACAAATGGCACGCTAAACCCCTTCCAATGGCTGACACATGAGATGAGTGACGGTATTCTGCAAGAGTGGCTAGCATTCATTTTGTCAATTTGTAGTCGTGACGAGTCACTGCAAACTTTTATGATGTACAGATTCCTGGAACAGGCAAAAATGACTGAAGCATTCTTTGCGGAACTCAAGGCTCGTCGGCAGAATTCGAATATTCAAACTATGTTGTCAAAGATCATCGATCTTGGTAGACATCAGCAGAGAATAGTTTCACAACAACATCAACAGCATCAAATGCAGCATCATCGACAACTAATGTGA | |||
| Experiment | Laboratory | JR | |||
| Date | 19 Oct 1998 00:00:00 | ||||
| Strain | WBStrain00022745 | ||||
| Treatment | Antisense wrm-1 RNA was injected into young JR663 adults at ~0.5 micrograms per microliter. Injected adults were incubated overnight at 20 degrees Celsius, collected on a fresh plate, and embryos laid between 18 and 24 hours were collected onto an agar pad and allowed to develop overnight without heat shock. Embryos laid after 24 hours were collected every 15 min, mounted onto an agar pad, and heat-shocked immediately for 30 min at 33-34C, followed by overnight incubation at 20 degrees Celsius. Gut granules, morphology, and MH33 reactivity were scored on developed embryos. | ||||
| Delivered_by | Injection | ||||
| Inhibits | Predicted_gene | B0336.1a | Inferred_automatically | RNAi_primary | |
| B0336.1b | Inferred_automatically | RNAi_primary | |||
| Gene | WBGene00006943 | Inferred_automatically | RNAi_primary | ||
| Transcript | B0336.1b.1 | Inferred_automatically | RNAi_primary | ||
| B0336.1a.1 | Inferred_automatically | RNAi_primary | |||
| Species | Caenorhabditis elegans | ||||
| Interaction | WBInteraction000520225 | ||||
| Reference | WBPaper00003328 | ||||
| Phenotype_not_observed | WBPhenotype:0001637 | Remark | Expression of the end-1 gene from heat shock promoters (in the JR663 strain) restored the presence of gut granules, gut-like cells, brush-border structures, and MH33 antigen expression after wrm-1 RNAi, indicating the successful production of intestinal cells. | ||
| Remark | (Figure 5) wrm-1 RNAi. Exact sequence used for RNAi not stated by authors, spliced coding region sequence of gene used for curation. | ||||
| Method | RNAi |
