WormBase Tree Display for RNAi: WBRNAi00090547
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WBRNAi00090547 | Homol | Homol_homol | B0041:RNAi | ||
---|---|---|---|---|---|
Sequence_info | PCR_product | sjj_B0041.5 | |||
Experiment | Date | 08 Sep 2011 00:00:00 | |||
Genotype | rrf-3(pk1426) | ||||
Treatment | Synchronized L1 larvae were grown for three days on plates seeded with RNAi bacteria until control worms (grown on empty-vector control plates) reached the L4 larval stage. L4 worms were washed off the RNAi plates with M9 buffer and Beta-Ala-Lys-AMCA was added to samples to a final concentration of 0.25 mM. Worms were incubated in this solution for 3 hours at 20 degrees Celsius. Liquid OP50 culture was added to the samples and incubated with the worms for 1 hour to wash the Beta-Ala-Lys-AMCA out of the intestinal lumen. Worms were washed 4-6 times in M9 buffer and assayed for intestinal uptake of Beta-Ala-Lys-AMCA by fluorescence microscopy. | ||||
Temperature | 20 | ||||
Delivered_by | Bacterial_feeding | ||||
Inhibits | Predicted_gene | B0041.5c | Inferred_automatically | RNAi_primary | |
B0041.5a | Inferred_automatically | RNAi_primary | |||
Gene | WBGene00015009 | Inferred_automatically | RNAi_primary | ||
WBGene00015011 | Inferred_automatically | RNAi_primary | |||
Transcript | B0041.5c.1 | Inferred_automatically | RNAi_primary | ||
B0041.5a.1 | Inferred_automatically | RNAi_primary | |||
B0041.8.1 | Inferred_automatically | RNAi_primary | |||
Species | Caenorhabditis elegans | ||||
Reference | WBPaper00040287 | ||||
Phenotype | WBPhenotype:0002082 | Remark | RNAi results in a decrease in intestinal uptake of the Beta-Ala-Lys-AMCA fluorescent dipeptide, compared to controls | ||
Affected_by | Molecule | WBMol:00005316 | |||
Phenotype_not_observed | WBPhenotype:0001182 | Remark | RNAi does not affect fatty acid uptake, as determined by uptake of BODIPY-C12, compared to controls | ||
Affected_by | Molecule | WBMol:00005316 | |||
Remark | (Table S1, Table 1) B0041.5 RNAi | ||||
Method | RNAi |