WormBase Tree Display for RNAi: WBRNAi00088918
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| WBRNAi00088918 | Homol | Homol_homol | W02A2:RNAi | ||
|---|---|---|---|---|---|
| Sequence_info | DNA_text | ATGGTTGTCTCTGCGGAAGTAATTCGAAAAGATGCCGTGTTTCACGTGGCTTGgtgagttttctcatcttttcaatctaatttataatttctgcttttccagGATTCTTGGTACCGGCGACTCGAAAAAGCTGTCACGAAGAGAGATTTTGGACCAAAATCTCCCGGAACTGTGTCATTCAATCATTGAAATGGTTCCCGAACGGCACAGGGGCTCAGCAACAAAAACTGGACTTTATTTGCTATCCCTGCTCACATATGGAACAGTTTTGATCCATCAAGTTCAGGTTGATTTCTTGAAACgtgagttggagaattgtatcgtcagaaatcaattaatattttcagGAGACGTCGAGAAGCTCAAAGAGCTCATGAAGAAGAAAAGTTTCATTTTGCTGATGGCTGAAAGGTTCGACAGAAACCAAGAGTTGCAGAGAAAAGAAGATAAATTCGCAAGACTGAGATCTAAGCCTATTATGTGCGTCGAGGAGTTGGATCGGGTTGATTTGGCTCATTTACAAGCAATTGGCGACGAATTGGGAgtgagttcaagttaattaaataatcaataatcaagaatattcttctagATCAATGGAAATCCAGGAGACTTTATAATGATGGATGCGTTGCCCAACATGAATCAGTGGATTGACAATAATTCAGAATTGAATGCGATCTATGGATGTGTTGAGCCTTACCTGAGgttcggaactgaataatcgatcccagattttgaaaaaaagcagcgacgcacatgaaccctgcggcacggtttttcaccttatttcaataaaatttcgattttccttgatttattcgcattttgcgtatgaaaatgctatccaattaaatttgcataagattgcgaggatatttcctgtttttacaaccaatgaagctctaaatatccttataatcttatgagaatttatgcggattgcatttccattcgaaaaaaagagaataaaccaattacaatcgaaattttattttaaaaaaatcaacaaaagctctgaaaaaaccgtgccacagggtagagaaattacactccgcctctatttctgtgcgacattgctttttgcaaaattgatggattgatagtttaattttaaattaatttcaaaagtaaaattttcgtgtttcagAGAAAAAGAAATCACCATGCACTCCACGTTTGTCGAGGGTAACGGTTCAAATGAGCACAACAAGGAACGACGAAATGACGCGGTAATTGCCGACTTCAGCCAACTTTTGTTCCCTGAGATCCCTGAAATCACACTTGGCGAGAAATTTCCGATAGATGTCGATTCCAGAAAACGATCTGCTATCCTCCAGGAAGAGCAAGAAGAGGCTCTTCAGCTCCCCAAAGAAGCTTCTGAAATTGTGCAAGAAGAGCCTACAAAATTTGTCTCTATTGCTCTGCTCCCGAGTGAAACCGTGGAACAACCAGCACCCCAGGAGCCTATTCAAGAGCCCATTCAACCAATTATCGAGGAGCCCGCGCCACAGCTTGAGCTTCCACAGCCAGAGCTTCCACCACAACTAGATGCAATTGATCTTGTCACCATTCCAGCGAGTCAGCAGGATATGGTTGTTGAATATCTTCAATTGATCAACGATTTACCCGATGACGAGAACAGCCGACTGCCACCATTGCCGAAAGATTTAGAATTGTTTGAAGATGTAATTCTACCACCTCCAGCAAAGAAATCAAAAGTCGAAGAGGAAGAAGACGCGTTGGAAAGAGCACGAAGAAGACCATCATCGCGTCCAGTAACTCCGATTAATCAAACCGATTTGACAGATCTTCATTCAACTGTGCGACCGGAAGATCCGAGTTTCGCAATAGACTCCCAAATTCATGATGTTCTACCTCAACGGAAAAAATCGAAACGCAATCTGCCGATCATCCACAGCGATGATCTGGAAATCGACGAAGCTGTGCAAAAAGTGTTACAAGCCGATTATTCCTCACTGGTTCGTAAGAAGGAGGATGTAATAGCAAAGATTCCACCAAAAACGGATGCAGTCGCGGTGCTCATGAACTTACCAGAACCAGTTTTCAGTATCGGATATCGTCTTCCTCCAGAGGTTCGGGATATGTTCAAAGCTTGTTATAATCAAGCTGTTGGAAGTCCAGTGAGCGATGATGAAGAGGATGAAGATGAGGAAGAAGAGGAAGAATATAAATATGCAAAAGTTTGTCTTCTGTCTCCAAATCGCATCGTTGAGGATACTCTACTTCTAGAAGAGCAACCTCGTCAACCTGAAGAATTTCCATCTACGGATAATATCAACCCGCCAAGACAGCTACAGGAGAATCCAGTTTTTGAGAATTTGGAATATGAAGCGCCCCCGCATCCAATAAGAACTGCTCGTACGCCCACGCCGATCAAAGATTTGAAATATTCAGTTATTAGTCTGTTCCCAACTCCAGAGAAACGCCGGGAGACGAGCATCATTGCTGAACTGAATTTGGATCCTATTCCAGTTGAAGAAATTGATCCACTTTTGACGATGAGAACCGAAGAAGAACTTGAAAATGTTCGACGCCGTCAAAAAAGCAGTTTAGGGGTTCAATTTATGAGAAgtgagatattttaatgtcgatttagtgttgaaaataatttttgccgaaaattgccgaaaatgtttaaattgggcagttctcgaattaccaaaaaaaaattccaattttcggaataaaaggtcaaaaatgtttcgattttttcctcgaaaatcgaaaattcttacattttcagagaatgaacgataaaaatcacattaaatcataaaatcaattttttttttgctcataaaaaaaataagaaaacatataattttattctttagaatatcttggatttctttaggtagaaaatgaccaaaattgttttaaaaaaataagcaaaccgatggtttgtgtcgggcttttggttttttttttactaaagtaaaaaacattatttttggatttttgaaaaatcgagaattttcgttgttcgatttttttgttatactcgccgattatttttcaaactttcggttgtcgcccccactttatagtttaaaataaaatattttcagCTGACGATCTGGAAGAGGATACTCGCCGTAATCGACTATTCGAGGATGAAGAACGGACTCGAGATGCTAGAGAAGATGAACTATTCTTCTATTCATCTGGAAGTCTCCTTCCCAATAATCGGCTCAACATCCACAAGGAATTGCTCAAgtgtgtatactaacactaaaacagaaagatcgacctgcaaattttgcaaggcgccgcacacaaaatgaggcggagcgtcgtttttttaaacttgcggctcgccagccattgtggaaaactgtaaataaatttttgtctaaaatgcttgatttcggataattttcgcttatttttttgtgtttaatattcgaaataatctaaaaatttggagtttattcaacgaaaatttgccattttcgccggaaaaataccataaatagtcagtttatttcgaatattaacaacaacgaaattagcgaaaatattccgaaatccagaattttcgcgaaaaattatttcatagttgtccacgagccgcaggcttgcagaacaacaccccgcctctttttctgtgcgacgcattggcaggtcaatcgctcagttttgatcgtaattggaactacgaaaaatcaaaaaaaaatcaatttttcagCGAAGCAGAAGCAAGATATCCAGAATGGGTGAATTTCAATGAATTCACTGCGGATCACGATCGTAAAAAGGCAGCAACCGCCTTTGAGGGCCTTCTTTgtaagatatttagtcgttgagacacaaattaaaataaaaataaatatttttcagTGAGTCTCAAGAATATGAAGGTTGAAGCGAAGCAAGAAGATCCTTATTTTCCAATTCTAGTACGTCACATCAGCCACGAAGAAATGTGA | |||
| Experiment | Laboratory | TY | |||
| Date | 26 Jun 2003 00:00:00 | ||||
| Delivered_by | Injection | ||||
| Inhibits | Predicted_gene | W02A2.6a | Inferred_automatically | RNAi_primary | |
| W02A2.6b | Inferred_automatically | RNAi_primary | |||
| W02A2.6c | Inferred_automatically | RNAi_primary | |||
| Gene | WBGene00004333 | Inferred_automatically | RNAi_primary | ||
| Transcript | W02A2.6a.1 | Inferred_automatically | RNAi_primary | ||
| W02A2.6b.1 | Inferred_automatically | RNAi_primary | |||
| W02A2.6c.1 | Inferred_automatically | RNAi_primary | |||
| Species | Caenorhabditis elegans | ||||
| Reference | WBPaper00005919 | ||||
| Phenotype | WBPhenotype:0000050 | Remark | RNAi caused complete embryonic lethality. Most nuclei of dying embryos had two 5S ribosomal DNA (rDNA) fluorescence in situ hybridization (FISH) signals, indicating normal chromosome segregation. Occasional nuclei had more than two FISH signals, perhaps from the premature dissociation of sister chromatids. | ||
| Phenotype_not_observed | WBPhenotype:0000773 | Remark | RNAi caused complete embryonic lethality. Most nuclei of dying embryos had two 5S ribosomal DNA (rDNA) fluorescence in situ hybridization (FISH) signals, indicating normal chromosome segregation. Occasional nuclei had more than two FISH signals, perhaps from the premature dissociation of sister chromatids. | ||
| Remark | paper remark: Exact sequence used for RNAi not stated by authors, genomic sequence of gene used for curation. Primers used were rec-8 5' TAATACGACTCACTATAGGGAATTCACTCCACGTTTGTCGAGGGTAAC 3' and 5' TAATACGACTCACTATAGGGCGGCCGCTAAACTGCTTTTTTGACGGCGTC. | ||||
| Method | RNAi |
