WormBase Tree Display for RNAi: WBRNAi00088895
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WBRNAi00088895 | Homol | Homol_homol | ZK270:RNAi | |||
---|---|---|---|---|---|---|
Sequence_info | PCR_product | sjj_ZK270.1 | ||||
Experiment | Laboratory | ALF | ||||
Date | 21 Mar 2011 00:00:00 | |||||
Genotype | daf-2(e1371); spe-9(hc88); fer-15(b26) | |||||
Treatment | Life-span assays were performed at 25 degrees Celsius with 25-30 eggs placed on two RNAi plates spotted with 2x concentrated RNAi of the gene of interest. The eggs were grown at 20 degrees Celsius until the L4 stage and then transferred to 25 degrees Celsius to obtain a synchronized adult population. The first day of adulthood was defined as Day 1. Animals were transferred to fresh plates every 2-4 days thereafter and examined daily for survival by looking for touch-provoked movement or pharyngeal pumping until all worms were dead or censored. | |||||
Temperature | 25 | |||||
Delivered_by | Bacterial_feeding | |||||
Inhibits | Predicted_gene | ZK270.1 | Inferred_automatically | RNAi_primary | ||
Gene | WBGene00004237 | Inferred_automatically | RNAi_primary | |||
Transcript | ZK270.1.1 | Inferred_automatically | RNAi_primary | |||
ZK270.1.2 | Inferred_automatically | RNAi_primary | ||||
Species | Caenorhabditis elegans | |||||
Reference | WBPaper00038430 | |||||
Phenotype_not_observed | WBPhenotype:0000039 | Remark | ptr-23/ZK270.1 RNAi of GL227 (daf-2(e1371); spe-9(hc88); fer-15(b26)) animals does not affect life span | |||
EQ_annotations | Life_stage | WBls:0000041 | PATO:0000460 | |||
Remark | ZK270.1 (ptr-23) RNAi | |||||
Method | RNAi |