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WormBase Tree Display for RNAi: WBRNAi00088895

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Name Class

WBRNAi00088895HomolHomol_homolZK270:RNAi
Sequence_infoPCR_productsjj_ZK270.1
ExperimentLaboratoryALF
Date21 Mar 2011 00:00:00
Genotypedaf-2(e1371); spe-9(hc88); fer-15(b26)
TreatmentLife-span assays were performed at 25 degrees Celsius with 25-30 eggs placed on two RNAi plates spotted with 2x concentrated RNAi of the gene of interest. The eggs were grown at 20 degrees Celsius until the L4 stage and then transferred to 25 degrees Celsius to obtain a synchronized adult population. The first day of adulthood was defined as Day 1. Animals were transferred to fresh plates every 2-4 days thereafter and examined daily for survival by looking for touch-provoked movement or pharyngeal pumping until all worms were dead or censored.
Temperature25
Delivered_byBacterial_feeding
InhibitsPredicted_geneZK270.1Inferred_automaticallyRNAi_primary
GeneWBGene00004237Inferred_automaticallyRNAi_primary
TranscriptZK270.1.1Inferred_automaticallyRNAi_primary
ZK270.1.2Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
ReferenceWBPaper00038430
Phenotype_not_observedWBPhenotype:0000039Remarkptr-23/ZK270.1 RNAi of GL227 (daf-2(e1371); spe-9(hc88); fer-15(b26)) animals does not affect life span
EQ_annotationsLife_stageWBls:0000041PATO:0000460
RemarkZK270.1 (ptr-23) RNAi
MethodRNAi