WormBase Tree Display for RNAi: WBRNAi00081731
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WBRNAi00081731 | Homol | Homol_homol | K08D9:RNAi | |||
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Sequence_info | DNA_text | caatatcacggagaagcccgcgaacgcattgatcttcacctcaaaattatcgaaccaacgagtaatgaaattctcgccctccaacaccaccgtcctgccgccgatacggaatggaactccgacgctccgatcgtcatcgagacctctcgtggcttcaacgtgacggttcaactgcggaatttgtgcagctcaaactaccacggaaaacgctgcaatcgatattgtatcgcgaatgcgaagcttcactgggagtgctcaacacacggagtccgccgatgctccgccggatggtctggtgaagattgttcaaatccgatctgcgccggcggttgcagcaatcgtggcagatgtgtggcaccgaaccaatgctcgtgcgccgacggattcaacggaactcgctgtgagcagtgcctaccacgcgcgggatgtgtcaacggagactgtgttaacgagacgccgaacacgtgcaagtgtagagacggttttattggtgatcggtgtgatattgacatcaaaatctgctctctcgaaaaaccgtgtgcaaacggtggaatctgttcaattgactcgtcatcgtcgaccggctacaaatgtcattgcccattcgaattcgtcggctctcaatgcaaaacaccgctgagcaaagttcggtgctccgcggagcacgtttgtaagaatggaggcgcttgcatctcaatggacgacacaaatattcagtgcaagtgccgtcgcggattttcgggaaaattctgtgaaattggaaatcacggggattgctcggcgatgcggtgctccgccggagagacgtgccaaattagtggagattttgcgatttgtgtggaaaatgatgagctactactgacaactaataagcctgctgaaacgacgaaaagcgttgaaaaatggagagaaccccgaaaaacggcgaacgacgagcaagcctcggatgagcttcaacttcggctgatcgccgcaatttgtgtgctcttttcagtttgtgtgatcggtctcgccttggtcagctttttcttctacatgcactcattctccaagtggaaacatccatcgtcacagcaggcaggaggaagcacaattcttccaacaacaacatcaattccaatgtctacaacgtcttcgggaactggatctcctgtttataaagtgtgcataatcgattcggagcaccgcggaaacgcgccaggaagctcatcagattccgaacccgatcaccactgtccgccgccgcaccgacattctccaccgcctgcctactcgagtctcgtcctgtacaaaaaagttccgatggcagcagatgacgagtcgtcgtttcgagtctaaacaacagctcgacgacgagatttacaataatttaataatgatttttttcgccaccaaatgcagatctttttcaaaaaaattttccctgtccctgtcccccgccacatcattttcagcccggttgttgctcttttcccatcattttcgctcaatgttttatcagattgtttatttttattattgctccctgttaacctcgattttctcactaaaaaggcgtcggggggctcttttttcccggaatttttaaggaagagtagagaacaatagccaaaaccggcctaatttcgtgctaaatttcgaaaatttggtgtgagggccaaattttcggaaaaagcacaaaatttggctctttttccaaaaatttttcccagtttttactcccttttaaaagcccaaattggcaaaattgagcccccaaatctacccagaaatcccaattttcgctctaaaacatctcccccagttttcagtctacgaggtatcttctgttttacacgactgttttttacgtctcattttgccatcaatttcaccactccacactcaaaaatgtaaatatttcgtccttatttttgcaccatttctctcccttttaaaatcgcattttttttcttgtatcattttctgcagtagttttttttgttgggtgtgtgtgctcattcattgattga | ||||
Experiment | Laboratory | LW | ||||
Date | 17 Oct 2007 00:00:00 | |||||
Strain | WBStrain00000001 | |||||
Treatment | Synchronized L1 worms at the 1-M stage were grown on RNAi feeding plates with the appropriate dsRNA expressing bacteria or soaked in 5x soaking buffer with double-stranded RNA corresponding to the gene of interest. Worms were soaked for 24 to 48 h at 16 C, then plated on either OP50 seeded NGM plates or RNAi-feeding plates and allowed to grow at the appropriate temperature. Adult worms were scored for their terminal M lineage phenotypes. | |||||
Delivered_by | Bacterial_feeding | |||||
Inhibits | Predicted_gene | K08D9.3f | Inferred_automatically | RNAi_primary | ||
K08D9.3a | Inferred_automatically | RNAi_primary | ||||
K08D9.3e | Inferred_automatically | RNAi_primary | ||||
Gene | WBGene00000168 | Inferred_automatically | RNAi_primary | |||
Transcript | K08D9.3d | Inferred_automatically | RNAi_primary | |||
K08D9.3b | Inferred_automatically | RNAi_primary | ||||
K08D9.3a.1 | Inferred_automatically | RNAi_primary | ||||
K08D9.3c | Inferred_automatically | RNAi_primary | ||||
K08D9.3f.1 | Inferred_automatically | RNAi_primary | ||||
K08D9.3e.1 | Inferred_automatically | RNAi_primary | ||||
Species | Caenorhabditis elegans | |||||
Reference | WBPaper00035079 | |||||
Phenotype_not_observed | WBPhenotype:0000414 | Remark | The ventral 4-M cells of the M-lineage (M.vlpa, M.vrpa) do NOT aberrantly produce coelomocytes instead of sex myoblasts | |||
EQ_annotations | Life_stage | WBls:0000041 | PATO:0000460 | |||
Method | RNAi |