WormBase Tree Display for RNAi: WBRNAi00070158
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WBRNAi00070158 | Homol | Homol_homol | C27H5:RNAi | ||
---|---|---|---|---|---|
F10E7:RNAi | |||||
Sequence_info | PCR_product | sjj_C27H5.4 | |||
Experiment | Laboratory | JF | |||
Date | 25 Jun 2007 00:00:00 | ||||
Genotype | mtl-2(gk125) | ||||
Treatment | Synchronized L1 larva were put on standard NGM plates with E. coli OP50 as food for approximately 31 h and allowed to develop into L4 larva. About 25 early L4 nematodes were then transferred to each well of a 6-well NGM plate, containing 1 mM IPTG and 25 mcg/ml carbenicillin, seeded with different RNAi expressing bacteria. Plates were then incubated at 20C for approximately 40 h. After the initial feeding period, 2 gravid adults from each well were then transferred to test plates containing RNAi bacteria and either 0 or 100 mcM cadmium. Each exposure was performed in triplicate. Control RNAi (empty vector) exposures were also performed for each cadmium treatment. Adults were allowed to lay eggs for approximately 12 h before removal. F1 nematodes were then grown on test plates for three additional days at 20C. At the end of the 3 day growth period, F1 nematodes were washed off the 6-well plates using M9 buffer and transferred onto 96-well plates. Body size measurements, as represented by nematode body length (time of flight (TOF)) were measured using the COPAS Biosort. | ||||
Temperature | 20 | ||||
Delivered_by | Bacterial_feeding | ||||
Inhibits | Predicted_gene | C27H5.4c | Inferred_automatically | RNAi_primary | |
C27H5.4a | Inferred_automatically | RNAi_primary | |||
C27H5.4b | Inferred_automatically | RNAi_primary | |||
Gene | WBGene00016174 | Inferred_automatically | RNAi_primary | ||
Transcript | C27H5.4c.1 | Inferred_automatically | RNAi_primary | ||
C27H5.4a.1 | Inferred_automatically | RNAi_primary | |||
C27H5.4b.1 | Inferred_automatically | RNAi_primary | |||
Species | Caenorhabditis elegans | ||||
Reference | WBPaper00030811 | ||||
Phenotype | WBPhenotype:0000031 | Remark | Difference between normalized mean body lengths of RNAi-treated animals exposed and not exposed to cadmium = 0.08 | ||
WBPhenotype:0001655 | Remark | Weak protective effects against cadmium toxicity. | |||
Method | RNAi |