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WBPicture0000014331Descriptionc-e Proapoptotic genes egl-1 and ced-3 are not highly expressed in the surviving B.al/rapaav homolog (c) but are highly expressed in the dyingB.al/rapaav (d). These two images were taken from the same animal, approximately 1.6 um apart. Anterior, left; dorsal, top. Scale bars: 10 um. Full Z-stack of this animal is available as Additional file 2: Movie 2, and two more examples are available as Additional file 3: Movie 3, Additional file 4: Movie 4. These animals were of the genotype nIs343; him-5(e1490); nIs349. e Quantification of nuclear mRNA transcripts of egl-1 and ced-3 in B.al/rapaav and the B.al/rapaav homolog. Only nuclear transcripts were counted, because we could not unambiguously determine to which cellnon-nuclear transcripts belonged. Condensed chromatin (as visualized by DAPI, which stains DNA and shows the chromatin in a dying cell to beslightly brighter and smaller in volume than that in a living cell), a position to the left or right of P12.pa, or expression of Pegl-1::4xNLS::gfp was each interpreted as indicating the secondary cell fate. All animals were of the genotype nIs343, him-5(e1490), nIs349. *: P < 0.005, **: P < 5 10-4
NameFig2_B.jpg
CropCropped_fromWBPicture0000014329
DepictExpr_patternExpr13315
Expr13316
AnatomyWBbt:0007050
WBbt:0007064
AcknowledgmentTemplateFrom: <Journal_URL> <Article_URL>. Copyright <Publication_year> <Publisher_URL>.
Publication_year2016
Article_URLDOIid10.1186/s12915-016-0262-5
Journal_URLBMCBiology
Publisher_URLBiomedCentralLTD
ReferenceWBPaper00049679